Abstract
Direct tubular effects of arginine vasopressin (AVP) on water and NaCl transport across the medullary thick ascending limb of Henle (MAL) were examined by the in vitro perfusion of isolated nephron fragments of mice, rats, and rabbits. Osmotic water permeability of the MAL of mice and rats was low and remained unchanged with 2 mU/ml AVP added to the bath. A dose-dependent increase in transepithelial electrical potential difference (PD) with AVP was observed in the mouse MAL when the ambient medium was isotonic. A similar result was also obtained when 2×10−4 mol/l dibutyryl adenosine 3′,5′-cyclic-monophosphate was added to the bath. In this preparation, AVP also caused an increase in the unidirectional Cl efflux from 323±45 to 398±61 pmoles·mm−1 ·min−1 (n=6,P<0.05). In contrast, under similar condition, we could not demonstrate any effect of AVP on PD, Cl efflux, or net Na flux in the rat MAL and on PD and Cl efflux in the rabbit MAL. Both PD and Cl efflux in the rat MAL were unaffected by AVP when the perfusate was made hypotonic. However, when the ambient medium was made hypertonic by adding NaCl and urea, a significant increase in PD was observed. In addition, we confirmed that AVP stimulated adenylate cyclase activity in the MAL as well as in the collecting tubule of mice and rats. We conclude that AVP stimulates Cl transport across the MAL of mice and rats by activating adenylate cyclase-cyclic AMP system. However, this effect of AVP may quantitatively vary among species.
Similar content being viewed by others
References
Antoniou, L. D., Burke, T. J., Robinson, R. R., Clapp, J. R.: Vasopressin-related alterations of sodium reabsorption in the loop of Henle. Kidney Int.3, 6–13 (1973)
Barry, P. H., Diamaond, J. M.: Junction potentials, electrode standard potentials, and other problems in interpreting electrical properties of membrane. J. Membr. Biol.3, 93–122 (1970)
Burg, M. B., Green, N.: Function of the thick ascending limb of Henle's loop. Am. J. Physiol.224, 659–668 (1973)
Burg, M. B., Grantham, J. J., Abramow, M., Orloff, J.: Preparation and study of fragments of single rabbit nephrons. Am. J. Physiol.210, 1293–1298 (1966)
Chabardès, D., Imbert-Teboul, M., Gagnan-Brunette, M., Morel, F.: Different hormonal target sites along the mouse and rabbit nephrons. In: Biochemical nephrology (W. G. Guder, U. Schmidt, eds.), pp. 447–454. Bern: Huber 1978
Chalfie, M., Neufeld, A. H., Zadunaisky, J. A.: Action of epinephrine and other cyclic AMP-mediated agents on the chloride transport of the frog cornea. Invest. Ophthalmol.11, 644–650 (1972)
Field, M.: Ion transport in rabbit ileal mucosa. II. Effects of cyclic 3′,5′-AMP. Am. J. Physiol.221, 992–997 (1971)
Fourman, J., Kennedy, G. C.: An effect of antidiuretic hormone on the flow of blood through the vasa recta of the rat kidney. J. Endocrinol.35, 173–176 (1966)
Frindt, G., Burg, M. B.: Effect of vasopressin on sodium transport in renal cortical collecting tubules. Kidney Int.1, 224–231 (1972)
Grantham, J. J., Burg, M. B.: Effect of vasopressin and cyclic AMP on permeability of isolated collecting tubules. Am. J. Physiol.211, 255–259 (1966)
Grantham, J. J., Orloff, J.: Effect of prostaglandin E1 on the permeability response of the isolated collecting tubule to vasopressin, adenosine 3′,5′-monophosphate, and theophylline. J. Clin. Invest.47, 1154–1161 (1968)
Hail, D. A.: Possible role of vasopressin in regulating solute transport in mouse medullary thick ascending limb of Henle's loop. Clin. Res.27, 416A (1979)
Hall, D. A., Barnes, L. D., Dousa, T. P.: Cyclic AMP in action of antidiuretic hormone: Effect of exogenous cyclic AMP and its new analogues. Am. J. Physiol.232, F368-F376 (1977)
Humphreys, M. H., Friedler, R. M., Earley, L. E.: Natriuresis produced by vasopressin or hemorrhage during water diuresis in the dog. Am. J. Physiol.216, 658–664 (1970)
Imbert, M., Chabardès, D., Montégut, M., Clique, A., Morel, F.: Adenylate cyclase activity along the rabbit nephron as measured in single isolated segments. Pflügers Arch.354, 213–228 (1975)
Imbert, M., Chabardès, D., Montégut, M., Clique, A., Morel, F.: Vasopressin dependent adenylate cyclase in single segments of rabbit kidney tubule. Pflügers Arch.357, 173–186 (1975)
Imbert-Teboul, M., Chabardès, D., Montégut, M., Clique, A., Morel, F.: Vasopressin-dependent adenylate cyclase activities in the rat kidney medulla: Evidence for two separate sites of action. Endocrinology102, 1254–1261 (1978)
Iino, Y., Imai, M.: Effects of prostaglandins on Na transport in isolated collecting tubules. Pflügers Arch.373, 125–132 (1978)
Imai, M.: Function of the thin ascending limb of Henle of rats and hamsters perfused in vitro. Am. J. Physiol.232, F201-F209 (1977)
Imai, M.: Effect of bumetanide and furosemide on the thick ascending limb of Henle's loop of rabbits and rats perfused in vitro. Eur. J. Pharmacol.41, 409–416 (1977)
Imai, M.: The connecting tubule: A functional subdivision of the rabbit distal nephron segments. Kidney Int.15, 346–356 (1979)
Klyce, S. D., Neufeld, A. H., Zadunaisky, J. A.: The activation of chloride transport by epinephrine and Db cyclic-AMP in the cornea of the rabbit. Invest. Ophthalmol.12, 127–139 (1973)
Leaf, A.: Membrane effects of anti-diuretic hormone. Am. J. Med.42, 745–756 (1967)
Martinez-Maldonado, M., Eknoyan, G., Suki, W. N.: Natriuretic effects of vasopressin and cyclic AMP: Possible site of action in the nephron. Am. J. Physiol.220, 2013–2020 (1971)
Morel, F.: Action of neurohypophyseal hormones on the active transport of sodium. In: Water and electrolyte metabolism (II) (J. de Graeff, B. Leijnse, eds.), pp. 91–104. Amsterdam: Elsevier 1964
Morel, F., Chabardés, D., Imbert, M.: Functional segmentation of the rabbit distal tubule by microdetermination of hormone-dependent adenylate cyclase activity. Kidney Int.9, 264–277 (1976)
Orloff, J., Handler, J.: The similarity of vasopressin, adenosine 3′,5′-phosphate (cyclic AMP) and theophylline on the toad bladder. J. Clin. Invest.41, 702–709 (1962)
Powell, D. W., Farris, R. K., Carbonetto, S. T.: Theophylline, cyclic AMP, choleragen, and electrolyte transport by rabbit ileum. Am. J. Physiol.227, 1428–1435 (1974)
Rocha, A. S., Kakko, J. P.: Sodium chloride and water transport in the medullary thick ascending limb of Henle. Evidence for active chloride transport. J. Clin. Invest.52, 612–623 (1973)
Rocha, A. S., Kokko, J. P.: Permeability of medullary nephron segments to urea and water: Effect of vasopressin. Kidney Int.6, 379–387 (1974)
Sawyer, W. H.: Posterior pituitary extracts and excretion of electrolytes by the rat. Am. J. Physiol.169, 583–587 (1952)
Sasaki, S., Imai, M.: Effect of vasopressin on the function of medullary thick ascending limb of Henle perfused in vitro. Jpn. J. Pharmacol. (abstr. in press, 1980)
Schäfer, J. A., Andreoli, T. E.: Cellular constraints to diffusion. J. Clin. Invest.51, 1264–1278 (1972)
Schnermann, J., Valtin, H., Thurau, K., Nagel, W., Horster, M., Fischbach, H., Wahl, M., Liebau, G.: Micropuncture studies on the influence of antidiuretic hormone on tubular fluid reabsorption in rats with hereditary hypothalamic diabetes insipidus. Pflügers Arch.306, 103–118 (1969)
Thurau, K., Deetjen, P., Kramer, K.: Hämodynamik des Nierenmarkes. II. Wechselbeziehung zwischen vasculären und tubulären gegenstromsystem arteriellen Drucksteigerungen, Wasserdiurese und osmotischer Diurese. Pflügers Arch.207, 270–285 (1960)
Torikai, S., Imai, M.: A simple method to determine adenylate cyclase activity in isolated single nephron segments by radioimmunoassay for succinyl adenosine 3′,5′-cyclic monophosphate. Tohoku J. Exp. Med.129, 91–99 (1979)
Ussing, H. H., Zerahn, K.: Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol. Scand.23, 110–127 (1951)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sasaki, S., Imai, M. Effects of vasopressin on water and NaCl transport across the in vitro perfused medullary thick ascending limb of Henle's loop of mouse, rat, and rabbit kidneys. Pflugers Arch. 383, 215–221 (1980). https://doi.org/10.1007/BF00587521
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00587521