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Pflügers Archiv

, Volume 386, Issue 3, pp 245–250 | Cite as

Agglutination kinetics of normal and diabetic adult rat hepatocytes

  • Anwar B. Bikhazi
  • Elias H. Abboud
  • Samuel K. Agulian
  • Camille F. Nassar
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

This paper describes Concanavalin A-induced agglutionation of viable rat hepatocytes obtained by collagenase perfusion from normal and streptozocin-treated diabetic rats. An irreversible cell-to-cell agglutination model is proposed to explain hepatocyte flocculation. The rate of agglutination is concentration dependent with respect to Concanavalin A, and is twice as fast in normal as compared to diabetic hepatocytes. Sticking probability constants ranging from 18.48×107 to 4.6×107 cm−1 · hepatocyte−1 and 8.32×107 to 2.31×107 cm−1 · hepatocyte−1 are calculated as a measure of agglutination for normal and diabetic cells respectively. These findings suggest that the cytoplasmic membranes of normal cells possess agglutinin receptors which are more numerous and/or differently arranged than those existing in diabetic cells.

Key words

Rat hepatocytes Streptozocin-induced diabetes Flocculation Sticking probability constant Agglutinin receptors Concanavalin A 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Anwar B. Bikhazi
    • 1
  • Elias H. Abboud
    • 1
  • Samuel K. Agulian
    • 1
  • Camille F. Nassar
    • 1
  1. 1.Department of Physiology, Faculty of MedicineAmerican University of BeirutBeirutRepublic of Lebanon

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