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Physical map of theHLA-A/HLA-F subregion and identification of two new coding sequences

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Abstract

As part of an effort to characterize the hemochromatosis gene, we selected three non-chimeric yeast artificial chromosomes (YACs) overlapping with the YAC B30 previously described and forming an 800 kilobase contig covering theHLA-A/HLA-F region. The precise physical map of these YACs and of the corresponding genomic region were established. Nine concentrated sites of CpG cutter elements, potentially HTF islands, were mapped. In addition, several probes have been generated as tools for mapping and examining transcripts produced in the region. This allowed for the characterization and localization of two new coding sequences, provisionally namedHCG (for hemochromatosis candidate gene) and numberedVIII andIX.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, UPR 41 CNRS — «Recombinaisons génétiques» Faculté de Médecine, 2 avenue du Professeur Léon Bernard, 35043, Rennes Cedex, France

    Laurent Pichon, Thierry Giffon, Bruno Chauvel, Gwenaelle Carn, Pascale Bouric, Abdel El Kahloun, Jean Yves Legall & Veronique David

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  1. Laurent Pichon
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  2. Thierry Giffon
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  3. Bruno Chauvel
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  4. Gwenaelle Carn
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  5. Pascale Bouric
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  6. Abdel El Kahloun
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  7. Jean Yves Legall
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  8. Veronique David
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the EMBL nucleotide sequence database and have been assigned the accession numbers X92109 (HCG VIII) and X92110 (HCGIX).

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Pichon, L., Giffon, T., Chauvel, B. et al. Physical map of theHLA-A/HLA-F subregion and identification of two new coding sequences. Immunogenetics 43, 175–181 (1996). https://doi.org/10.1007/BF00587297

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  • DOI: https://doi.org/10.1007/BF00587297

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