Summary
The O2 diffusion field within an agarose medium in front of a 1.5 mm platinum macroelectrode with plane and circular surface was measured by means of a platinum microelectrode (Pt diameter of ca. 0.3 μm). The\(P_{O_2 }\) profiles measured in different parts of the O2 diffusion field agree very well with the theoretically calculated values. Under conditions in which the O2 flow in the medium is high as compared with the O2 used up by the microelectrode, the results show that local\(P_{O_2 }\) can be measured correctly by the microelectrode. After the theory of the polarographic step it is assumed that the oxygen partial pressure at the platinum surface equals zero. The coincidence of the measured and calculated values allows the conclusion that the assumption of\(P_{O_2 }\)=0 at the platinum surface is correct. The flow density at the Pt surface of the macroelectrode can be demonstrated very well by the deposit of palladium.
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Baumgärtl, H., Grunewald, W. & Lübbers, D.W. Polarographic determination of the oxygen partial pressure field by Pt microelectrodes using the O2 field in front of a Pt macroelectrode as a model. Pflugers Arch. 347, 49–61 (1974). https://doi.org/10.1007/BF00587054
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DOI: https://doi.org/10.1007/BF00587054