Pflügers Archiv

, Volume 336, Issue 4, pp 311–325 | Cite as

Changes of protein synthesis in the hypertrophying rat heart

  • Heinz-Gerd Zimmer
  • Gudrun Steinkopff
  • Eckehart Gerlach
Article

Summary

Myocardial protein synthesis was studied in rats in vivo during the first five days of the development of cardiac hypertrophy induced by aortic constriction. Using l-14C-glycine or l-14C-leucine as precursor amino acids, rates of protein synthesis were determined from the total radioactivity of proteins and the mean radioactivity of the intracellular amino acid precursor pool and the leucine pool, respectively. During the first 5 h after aortic constriction the radioactivity of proteins did not change remarkably, whereas the radioactivity of the amino acid precursor pool was significantly elevated. Myocardial protein synthesis proved therefore to be diminished in this initial phase. After 24 h protein synthesis reached the range of sham-operated controls and thereafter increased almost parallel with the elevation of the ratio heart weight/body weight. The inhibition of protein synthesis was accompanied by a moderate decrease of ATP and creatine phosphate levels. A diminution of the high energy phosphate compounds, a decreased RNA synthesis or the action of inhibitory metabolites are considered possible factors involved in the decline of protein synthesis during the early phase of cardiac hypertrophy.

Key words

Aortic Constriction Cardiac Hypertrophy Amino Acid Incorporation Protein Synthesis 

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • Heinz-Gerd Zimmer
    • 1
  • Gudrun Steinkopff
    • 1
  • Eckehart Gerlach
    • 1
  1. 1.Abteilung Physiologie, Medizinische FakultätTechnische Hochschule AachenAachenGermany

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