Pflügers Archiv

, Volume 379, Issue 2, pp 215–218 | Cite as

Development of pump electrogenesis in hypokalaemic rat muscle

  • Norio Akaike
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine glands

Abstract

In hypokalaemic rats maintained on a potassium deficient diets for 10–50 days, the isolated ‘Na-loaded’ and ‘K-depleted’ (‘Na-rich’) muscle fibers showed the membrane potential less than −115 mV in ‘fresh’ muscles of normal rats in K+-free Krebs solution. Upon adding 5 mM K+ to the K+-free medium bathing the soleus muscles, the measured potentials of ‘Na-rich’ muscles always exceeded the membrane potentials of ‘fresh’ muscles in 5 mM K+. The hyperpolarization was dependent on the amount of intracellular Na+ concentration ([Na]i) accumulated during the potassium deficiency. The electrogenic Na-pump was activated by an increase of [Na]i of less than 5 mM. Further increases in [Na]i resulted in increases in membrane potential which appeared to approach a limit at [Na]i levels higher than 65 mM.

Key words

Hypokalaemic rat Soleus muscle [Na]i and Na-pump 

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References

  1. Adrian, R. H., Slayman, C. L.: Membrane potential and conductance during transport of sodium, potassium and rubidium in frog muscle. J. Physiol. (Lond.)184, 970–1014 (1966)Google Scholar
  2. Akaike, N.: Operation of an electrogenic Na-pump in mammalian red muscle fibre. Life Sci.14, 141–147 (1974)Google Scholar
  3. Akaike, N.: Contribution of an electrogenic sodium pump to membrane potential in mammalian skeletal muscle fibres. J. Physiol. (Lond.)245, 499–520 (1975a)Google Scholar
  4. Akaike, N.: Activation of electrogenic sodium pump in mammalian skeletal muscle by external cations. Pflügers Arch.355, 281–290 (1975b)Google Scholar
  5. Akaike, N.: Intracellular ion concentration and electrical activity in potassium-depleted mammalian soleus muscle fibres. Pflügers Arch.362, 15–20 (1976)Google Scholar
  6. Akiyama, T., Grundfest, H.: The hyperpolarization of frog skeletal muscle fibres induced by removing potassium from the bathing medium. J. Physiol. (Lond.)217, 33–60 (1971)Google Scholar
  7. Desmedt, J. E.: Electrical activity and intracellular sodium concentration in frog muscle. J. Physiol. (Lond.)121, 191–205 (1953)Google Scholar
  8. Dockry, M., Kernan, R. P., Tangney, A.: Active transport of sodium and potassium in mammalian skeletal muscle and its modification by nerve and by cholinergic and adrenergic agents. J. Physiol. (Lond.)186, 187–200 (1966)Google Scholar
  9. Erdmann, E., Bolte, H. D., Lüderitz, B.: The (Na++K+)-ATPase activity of guinea pig heart muscle in potassium deficiency. Arch. Biochem. Biophys.145, 121–125 (1971)Google Scholar
  10. Frumento, A. S.: Sodium pump: Its electrical effects in skeletal muscle. Science (N.Y.)147, 1442–1443 (1965)Google Scholar
  11. Glitsch, H. G.: Activation of the electrogenic sodium pump in guinea-pig auricles by internal sodium ions. J. Physiol. (Lond.)220, 565–582 (1972)Google Scholar
  12. Hashimoto, Y.: Resting potentials of Na-loaded sartorius muscle fibres of toads during recovery in high K Ringer. Kumamoto med. J.18, 23–30 (1965)Google Scholar
  13. Hiraoka, M., Hecht, H. H.: Recovery from hypothermia in cardiac Purkinje fibers: Considerations for an electrogenic mechanism. Pflügers Arch.339, 25–36 (1973)Google Scholar
  14. Kernan, R. P., Tangney, A.: An electrogenic Na-pump in frog striated muscle. J. Physiol. (Lond.)172, 32P (1964)Google Scholar
  15. Kobayashi, N., Yonemura, K.: The extracellular space in red and white muscles of the rat. Jap. J. Physiol.17, 698–707 (1967)Google Scholar
  16. Noma, A., Irisawa, H.: Contribution of an electrogenic sodium pump to the membrane potential in rabbit sinoatrial node cells. Pflügers Arch.358, 289–301 (1975)Google Scholar
  17. Sato, M., Akaike, N., Nishi, R.: Membrane potentials of frog sartorius muscle fibres, in which potassium ions were replaced by sodium. Kumamoto med. J.20, 39–55 (1967)Google Scholar
  18. Tamai, K., Kagiyama, S.: Studies of cat heart muscle during recovery after prolonged hypothermia. Hyperpolarization of cell membranes and its dependence on the sodium pump with electrogenic characteristics. Circulat. Res.22, 423–433 (1968)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Norio Akaike
    • 1
  1. 1.Department of Physiology and BiophysicsUniversity of Texas Medical BranchGalvestonUSA

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