Pflügers Archiv

, Volume 379, Issue 2, pp 215–218 | Cite as

Development of pump electrogenesis in hypokalaemic rat muscle

  • Norio Akaike
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine glands


In hypokalaemic rats maintained on a potassium deficient diets for 10–50 days, the isolated ‘Na-loaded’ and ‘K-depleted’ (‘Na-rich’) muscle fibers showed the membrane potential less than −115 mV in ‘fresh’ muscles of normal rats in K+-free Krebs solution. Upon adding 5 mM K+ to the K+-free medium bathing the soleus muscles, the measured potentials of ‘Na-rich’ muscles always exceeded the membrane potentials of ‘fresh’ muscles in 5 mM K+. The hyperpolarization was dependent on the amount of intracellular Na+ concentration ([Na]i) accumulated during the potassium deficiency. The electrogenic Na-pump was activated by an increase of [Na]i of less than 5 mM. Further increases in [Na]i resulted in increases in membrane potential which appeared to approach a limit at [Na]i levels higher than 65 mM.

Key words

Hypokalaemic rat Soleus muscle [Na]i and Na-pump 


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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Norio Akaike
    • 1
  1. 1.Department of Physiology and BiophysicsUniversity of Texas Medical BranchGalvestonUSA

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