Pflügers Archiv

, Volume 333, Issue 2, pp 156–165 | Cite as

Die Metabolisierung von p-Aminohippurat in Nieren von normalen Ratten und Ratten mit experimentellem Goldblatt-Hochdruck

  • M. Mályusz
  • J. Girndt
  • G. Mályusz
  • B. Ochwadt

The metabolism of p-aminohippurate in the kidney of normal rats and rats with experimental Goldblatt-Hypertension


PAH-clearance studies were carried out separately on each kidney of both normal rats and rats with experimental Goldblatt-Hypertension. The rat and especially the rat kidney metabolizes a certain proportion of PAH-molecules 1. by splitting off the glycine-group forming p-aminobenzoate (PAB); 2. both PAH and PAB undergo partial N-acetylation. N-acetylated metabolites cannot be detected by the conventional analysis of PAH. Resorption of benzoates in the kidney results in a relatively high benzoate concentration in the renal vein. The complications created by these facts are discussed in relation to RPF-estimation using PAH. A correct determination of RPF by PAH is possible only if the analysis is carried out after an acid hydrolysis to make the detection of N-acetylated metabolites possible and if renal venous blood samples can be collected to correct for the reabsorbed benzoates. The clamped kidney of rats with Goldblatt-Hypertension is able to split glycine off from PAH but its capacity to N-acetylate is reduced. As a result identical PAH-clearances are found in both kidneys of these animals although their true RPF differ significantly.

Key words

Kidney Goldblatt-Hypertension PAH-clearance PAH-Metabolism 


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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • M. Mályusz
    • 1
    • 2
    • 3
  • J. Girndt
    • 1
    • 2
  • G. Mályusz
    • 1
    • 2
  • B. Ochwadt
    • 1
    • 2
  1. 1.Abt. PhysiologieMax-Planck-Institut für experimentelle MedizinGöttingenGermany
  2. 2.Medizinische Klinik der Universität GöttingenGöttingenGermany
  3. 3.Physiologisches Institut II. LehrstuhlKiel 1Deutschland

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