Advertisement

Pflügers Archiv

, Volume 333, Issue 2, pp 156–165 | Cite as

Die Metabolisierung von p-Aminohippurat in Nieren von normalen Ratten und Ratten mit experimentellem Goldblatt-Hochdruck

  • M. Mályusz
  • J. Girndt
  • G. Mályusz
  • B. Ochwadt
Article

The metabolism of p-aminohippurate in the kidney of normal rats and rats with experimental Goldblatt-Hypertension

Summary

PAH-clearance studies were carried out separately on each kidney of both normal rats and rats with experimental Goldblatt-Hypertension. The rat and especially the rat kidney metabolizes a certain proportion of PAH-molecules 1. by splitting off the glycine-group forming p-aminobenzoate (PAB); 2. both PAH and PAB undergo partial N-acetylation. N-acetylated metabolites cannot be detected by the conventional analysis of PAH. Resorption of benzoates in the kidney results in a relatively high benzoate concentration in the renal vein. The complications created by these facts are discussed in relation to RPF-estimation using PAH. A correct determination of RPF by PAH is possible only if the analysis is carried out after an acid hydrolysis to make the detection of N-acetylated metabolites possible and if renal venous blood samples can be collected to correct for the reabsorbed benzoates. The clamped kidney of rats with Goldblatt-Hypertension is able to split glycine off from PAH but its capacity to N-acetylate is reduced. As a result identical PAH-clearances are found in both kidneys of these animals although their true RPF differ significantly.

Key words

Kidney Goldblatt-Hypertension PAH-clearance PAH-Metabolism 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. 1.
    Cross, R. J., Taggart, J. V.: Renal tubular transport: Accumulation of p-amino-hippurate by rabbit kidney slices. Amer. J. Physiol.161, 181 (1950).Google Scholar
  2. 2.
    Despopoulos, A.: In vitro effects of acetate ion on renal metabolism of p-amino-hippurate. Amer. J. Physiol.184, 396 (1956).Google Scholar
  3. 3.
    Ellinger, A., Heusel, M.: Quantitative Studien über Acetylierungsprozesse im Tierkörper. I. Mitteilung: Die Bildung von p-Acetylaminobenzoesäure aus p-Aminobenzaldehyd und p-Aminobenzoesäure. Hoppe-Seylers Z. physiol. Chem.91, 21 (1914).Google Scholar
  4. 4.
    Frindt, G., Vial, S.: Conjugation of p-aminohippuric acid by human kidney and liver slices. Acta physiol. lat.-amer.18, 55 (1968).Google Scholar
  5. 5.
    Führ, J., Kaczmarczyk, J., Krüttgen, C. D.: Eine einfache colorimetrische Methode zur Inulinbestimmung für Nieren-Clearance-Untersuchungen bei Stoffwechselgesunden und Diabetikern. Klin. Wschr.33, 729 (1955).Google Scholar
  6. 6.
    Girndt, J., Ochwadt, B.: Durchblutung des Nierenmarkes. Gesamtdurchblutung und cortico-medulläre osmotische Gradienten beim experimentellen Hochdruck der Ratte. Pflügers Arch.313, 30 (1969).Google Scholar
  7. 7.
    Knoefel, P. K., Huang, K. C., Despopoulos, A.: Conjugation and excretion of amino and acetamido benzoic acids. Amer. J. Physiol.196, 1224 (1959).Google Scholar
  8. 8.
    Lowitz, H. D., Stumpe, K. O., Ochwadt, B.: Natrium- und Wasserresorption in den verschiedenen Abschnitten des Nephrons beim experimentellen renalen Hochdruck der Ratte. Pflügers Arch.304, 322 (1968).Google Scholar
  9. 9.
    Patterson, M. S., Greene, R. C.: Measurement of low energy beta-emitters in aqueous solution by liquid scintillation counting of emulsions. Analyt. Chem.37, 854 (1965).Google Scholar
  10. 10.
    Riggs, T. R., Christensen, H. N.: Metabolic conjugations of p-aminobenzoic acid in the rat. J. biol. Chem.193, 675 (1951).Google Scholar
  11. 11.
    Setchell, B. P., Blanch, E.: Conjugation of p-aminohippurate by the kidney and effective renal plasma-flow. Nature (Lond.)189, 230 (1961).Google Scholar
  12. 12.
    Smith, H. W.: The kidney. Structure and function in health and disease. pp. 158–159. New York: Oxford University Press 1951.Google Scholar
  13. 13.
    —, Finkelstein, N., Aliminosa, L., Crawford, B., Graber, M.: The renal clearances of substituted hippuric acid derivatives and other aromatic acids in dog and man. J. clin. Invest.24, 388 (1945).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • M. Mályusz
    • 1
    • 2
    • 3
  • J. Girndt
    • 1
    • 2
  • G. Mályusz
    • 1
    • 2
  • B. Ochwadt
    • 1
    • 2
  1. 1.Abt. PhysiologieMax-Planck-Institut für experimentelle MedizinGöttingenGermany
  2. 2.Medizinische Klinik der Universität GöttingenGöttingenGermany
  3. 3.Physiologisches Institut II. LehrstuhlKiel 1Deutschland

Personalised recommendations