Summary
Extracts of healthy and diseased skin in vitiligo, naevi and malignant melanoma were electrophoresed on polyacrylamide gel. Melanin bands were visualized on the gel by Dopa reaction. The tyrosinases found in malignant melanoma were also present in naevocellular naevi. Additional dopa melanin bands were observed. Some bands which were not related to the presence of melanocytes varied with the level of the melanogenetic activity in the individual patients.
Zusammenfassung
Extrakte von gesunder und erkrankter Haut von Patienten mit Vitiligo, Naevi und malignem Melanom wurden mit Polyacrylamidgel-Elektrophorese untersucht. Die Melaninbanden wurden auf dem Gel durch die Dopa-Reaktion dargestellt. Die Tyrosinasen im malignen Melanom waren auch in Naevuszellnaevi sichtbar. Zusätzliche Melaninbanden wurden beobachtet. Einige Banden, welche nicht mit Melanocyten in Beziehung stehen, wechseln mit dem Grad melano-genetischen Aktivität der einzelnen Patienten.
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We are pleased to acknoledge the expert technical assistance of Mrs. Ch. Khatchadourian. This work was in part supported by I.N.S.E.R.M. grant (No. CRL 75-1.172.2).
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Voulot, C., Ortonne, JP. The tyrosinases of normal and deseased human skin. Arch. Derm. Res. 254, 205–214 (1975). https://doi.org/10.1007/BF00586894
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DOI: https://doi.org/10.1007/BF00586894