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Funktionelle und morphologische Untersuchungen am proximalen und distalen Konvolut der Rattenniere zur Methode der gespaltenen Ölsäule (Split-Oil Droplet Method)

Functional and morphologic investigation of proximal and distal convolutions of rat kidney under the influence of the split-oil droplet method

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Summary

Micropuncture experiments have been carried out in proximal and distal convolutions of the rat kidney in order to test the reliability of the split-oil droplet method.

  1. 1.

    Light- and electronmicroscopic results: In castor oil filled segments a sufficient blockade is achieved, as judged from the compression of the brush border. In the test segments, interposed between two oil filled segments which are injected with ferritin containing saline, the tubular epithelium and especially the luminal brush border exhibit a normal histology. The tight junctions are intact and pinocytosis is indicated by ferritin resorption. Only in very few instances were oil droplets found to adhere to the brush border of the test segment, sometimes forming circumscript deposits protruding towards the cells. In such areas mechanical lesions of the cell membrane were only very rarely observed.

    In the proximal and distal convolutions of adrenalectomized rats the same experimental procedure did not lead to differences in the morphology of the epithelium.

  2. 2.

    Physiological data:

    1. a)

      Half time of volume reabsorption was unchanged when the split-oil droplet procedure was applied repeatedly to the same tubular segment.

    2. b)

      Intraluminal application of KCN (10−3–10−2 Mol/l) inhibited reabsorption of NaCl and fluid out of the test segment.

    3. c)

      Addition of the non-permeating polyethyleneglycol to the test solution prolongedt1/2 to infinity.

    4. d)

      In experimental diabetes insipidus half time of fluid reabsorption was greatly increased in the distal convolution, but could be normalized by intravenous injection of vasopressin. This was true even when the tubular epithelium was in contact with castor oil for more than 60 min prior to the administration of the hormone.

    From the results obtained in this and earlier studies it is concluded that contact with castor oil and tubular dilatation (to the extent that occurs during the procedure of the split-oil droplet method) does not damage tubular epithelium to a measureable degree as judged both by functional and morphological criteria. This holds also for adrenalectomized rats.

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Authors and Affiliations

  1. Physiologisches Institut der Freien Universität Berlin, Berlin, Deutschland

    M. Wiederholt, K. H. Langer, W. Thoenes & K. Hierholzer

  2. Pathologisches Institut der Universität Würzburg, Würzburg, Deutschland

    M. Wiederholt, K. H. Langer, W. Thoenes & K. Hierholzer

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  4. K. Hierholzer
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Wiederholt, M., Langer, K.H., Thoenes, W. et al. Funktionelle und morphologische Untersuchungen am proximalen und distalen Konvolut der Rattenniere zur Methode der gespaltenen Ölsäule (Split-Oil Droplet Method). Pflugers Arch. 302, 166–191 (1968). https://doi.org/10.1007/BF00586868

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