Summary
14C-sodium ferrocyanide and3H inulin were simultaneously microinjected into surface convolutions of proximal tubules or cortical peritubular capillaries of the left kidney. Ureteral urine was fractionally collected from both kidneys after each injection in order to demonstrate the subsequent excretory pattern of each substance. The results of 25 intratubular injections revealed no significant difference between the excretory pattern of either indicator. The mean total recovery of3H inulin was not significantly different from that of14C-Na ferrocyanide (P for mean difference ≧0.5 pairedt test). However a comparison between the time of 50% excretion of each substance revealed a slight but significant delay in the excretion of ferrocyanide [0.086±0.03 min (SE)].
Each of the five cortical peritubular capillary microinjections gave rise to a similar excretory pattern for both indicators from each kidney; in every case the kinetic excretory pattern of14C ferrocyanide closely followed that of3H inulin. The mean ratio amount of ferrocyanide excreted/amount of inulin excreted, obtained for the experimental (left) kidney, did not differ significantly from that obtained for the control (right) kidney. (P for mean difference ≧0.2, pairedt test.)
It was concluded that after simultaneous microinjection into proximal tubules or cortical peritubular capillaries no significant difference exists between the renal handling of14C-Na ferrocyanide and3H-inulin, also no loss of ferrocyanide occured either into or through the cells bordering the tubular lumen.
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Moss, N.G., de Rouffignac, C. Comparison between the renal handling of14C-Na ferrocyanide and3H-methoxy inulin in the rat. Pflugers Arch. 338, 19–29 (1972). https://doi.org/10.1007/BF00586852
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DOI: https://doi.org/10.1007/BF00586852