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Physical properties of the rat renal brush border membrane during growth

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastroinestinal Tract, and Exocrine Glands
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Abstract

The biophysical properties of rat renal brush border membranes were examined during neonatal development and following unilateral nephrectomy by sensitive fluorescence techniques. Differences in the fluorscence anisotropy of 1,6 diphenyl-1,3,5-hexatriene (DPH) following unilateral nephrectomy were not apparent; by contrast, the luminal membrane becomes more rigid as animals age. Although growth of the kidneys is common to both states examined, fundamental differences are apparent at the level of the luminal membrane.

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References

  1. Segal S, Rosenhagen M, Rea C (1973) Developmental and other characteristics of α-methyl-D-glucoside transport by rat kidney cortex slices. Biochim Biophys Acta 291:519–530.

    Google Scholar 

  2. Goldman D R, Roth K S, Langfitt T W Jr, Segal S (1979) L-proline transport by newborn rat kidney brush border-membrane vesicles. Biochem J 178:253–256.

    Google Scholar 

  3. Lelievre-Pegorier M, Jean T, Ripoche P, Poujeol P (1983) Transport of phosphate, D-glucose, and L-valine in newborn rat kidney brush border. Am J Physiol 245 (Renal Fluid Electrolyte Physiol 14):F367-F373.

    Google Scholar 

  4. Larsson L (1975) The ultrastructure of the developing proximal tubule in the rat kidney. J Ultrastruct Res 51:119–139.

    Google Scholar 

  5. Anderson W A (1967) The fine structure of compensatory growth in the rat kidney after unilateral nephrectomy. Am J Anat 121:217–227.

    Google Scholar 

  6. Harris R C, Seifter J L, Brenner B M (1984) Adaptation of Na+−H+ exchange in renal microvillus membrane vesicles. J Clin Invest 74:1979–1987.

    Google Scholar 

  7. Amatruda J M, Finch E D (1979) Modulation of hexose uptake and insulin action by cell membrane fluidity. J Biol Chem 254:2619–2625.

    Google Scholar 

  8. Bikle D D, Whitney J, Munson S (1984) The relationship of membrane fluidity to calcium flux in chick intestinal brush border membranes. Endocrinology 114:260–267.

    Google Scholar 

  9. Aronson P S (1978) Energy-dependence of phlorizin binding to isolated renal microvillus membranes. J Membr Biol 42:81–98.

    Google Scholar 

  10. Blomstedt J W, Aronson P S (1980) pH gradient stimulated transport of urate and p-aminohippurate in dog renal microvillus membrane vesicles. J Clin Invest 65:931–934.

    Google Scholar 

  11. Kahn A M, Branham S, Weinman E J (1983) Mechanism of urate and p-aminohippurate transport in rat renal microvillus membrane vesicles. Am J Physiol 245 (Renal Fluid Electrolyte Physiol 14): F151-F158.

    Google Scholar 

  12. King T E (1967) Preparation of succinate-cytochrome a reductase. In: Eastbrook R W, Pullman M E, eds.Methods in Enzymology, New York: Academic Press, Vol. 10:216–255.

    Google Scholar 

  13. Wallach D F H, Kamat V B (1966) Preparation of plasma membrane fragments from mouse ascites tumor cells. In: Ginsburg E, Neufeld E, eds.Methods in Enzymology, New York: Academic Press, Vol. 8:164–172.

    Google Scholar 

  14. Lowry O H, Rosebrough N J, Farr A L, Randall R J (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275.

    Google Scholar 

  15. Shinitzky M, Barenholz Y (1978) Fluidity parameters of lipid regions determined by fluorescence polarization. Biochim Biophys Acta 515:367–394.

    Google Scholar 

  16. Hise M K, Mantulin W W, Weinman E J (1984) Fluidity and composition of brush border and basolateral membranes from rat kidney. Am J Physiol 247 (Renal Fluid Electrolyte Physiol 16):F434-F439.

    Google Scholar 

  17. Bruscalupi G, Curatola G, Lenaz G, Leoni S, Mangiantini M T, Mazzanti L, Spagnuolo S, Trentalance A (1980) Plasma membrane changes associated with rat liver regeneration. Biochim Biophys Acta 597:263–273.

    Google Scholar 

  18. Hitzmann R J, Johnson D A (1983) Developmental changes in synaptic membrane lipid composition and fluidity. Neurochem Res 8:121–131.

    Google Scholar 

  19. Schwarz S M, Ling S, Hostetler B, Draper J P, Watkins J B (1984) Lipid composition and membrane fluidity in the small intestine of the developing rabbit. Gastroenterology 86:1544–1551.

    Google Scholar 

  20. Goldmann D R, Schlesinger H, Segal S (1976) Isolation and characterization of the brush border fraction from newborn rat renal proximal tubule cells. Biochim Biophys Acta 419:251–260.

    Google Scholar 

  21. Lajeunesse D, Carriere B, Giocondi M C, LeGrimellac C (1984) Modification of physiochemical properties of rat kidney brush border membrane upon aging. Kidney Int 25:306.

    Google Scholar 

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Authors and Affiliations

  1. Medical School, Division of Nephrology, The University of Texas, MSMB 4.130, 77225, Houston, TX, USA

    M. K. Hise & E. J. Weinman

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  1. M. K. Hise
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  2. E. J. Weinman
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Hise, M.K., Weinman, E.J. Physical properties of the rat renal brush border membrane during growth. Pflugers Arch. 406, 234–236 (1986). https://doi.org/10.1007/BF00586689

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  • DOI: https://doi.org/10.1007/BF00586689

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