Advertisement

Pflügers Archiv

, Volume 406, Issue 2, pp 158–162 | Cite as

ATP-evoked membrane responses inXenopus oocytes

  • Ilana Lotan
  • Nathan Dascal
  • Sasson Cohen
  • Yoram Lass
Excitable Tissues and Central Nervous Physiology

Abstract

Voltage-clamp technique and intracellular injections of drugs were used to study the adenosine triphosphate (ATP)-evoked depolarizing current response in theXenopus laevis oocytes. The depolarizing current was comprised of a fast transient component (D1) followed by a late long-lasting component (D2). It was carried mainly by Cl ions. The depolarizing current was better elicited by ATP and ADP than by AMP or adenosine and was not blocked either by theophylline (0.2 mM) or by quinidine sulphate (1 mM). The D2 current was sometimes masked by an ATP-evoked K+ hyperpolarizing current which was blocked by theophylline and mediated via P1 purinoceptors. This study suggests that the oocyte's membrane embodies at least two different purinoceptor's types, each of these types subserves a different set of ionic channels.

Key words

ATP Oocyte Purinoceptors 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barish ME (1983) A transient calcium-dependent chloride current in the immatureXenopus oocyte. J Physiol 342:309–325Google Scholar
  2. Barnard EA, Miledi R, Sumikawa K (1982) Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces junctional receptors inXenopus oocytes. Proc R Soc Lond (Biol) 215:241–246Google Scholar
  3. Bowman WC, Hall MT (1970) Inhibition of rabbit intestine mediated by alfa- and beta-adrenoceptors. Br J Pharmacol 38:399–415Google Scholar
  4. Burgess GM, Claret M, Jenkinson DH (1979) Effects of catecholamines, ATP and ionophore A23187 on potassium and calcium movements in isolated hepatocytes. Nature 279: 544–546Google Scholar
  5. Burnstock G (1978) A basis for distinguishing two types of purinergic receptor. In: Straub RW, Bolis L (eds) Cell membrane receptors for drugs and hormones: a multidisciplinary approach. Raven Press, New York, pp 107–118Google Scholar
  6. Burnstock G (1981) Neurotransmitters and trophic factors in the autonomic nervous system. J Physiol 313:1–34Google Scholar
  7. Burnstock G (1985) Purinergic mechanisms broaden their sphere of influence. TINS 1:5–6Google Scholar
  8. Dascal N, Landau EM (1980) Types of muscarinic responses inXenopus oocytes. Life Sci 27:1423–1428Google Scholar
  9. Dascal N, Landau EM (1982) Cyclic GMP mimics the muscarinic response inXenopus oocytes: identity of ionic mechanisms. Proc Natl Acad Sci USA 79:3052–3056Google Scholar
  10. Dascal N, Landau EM, Lass Y (1984)Xenopus oocytes resting potential, muscarinic responses and the role of calcium and guanosine 3′, 5′ cyclic monophosphate. J Physiol 352:551–574Google Scholar
  11. Dascal D, Gillo B, Lass Y (1985) Role of calcium mobilization in mediation of acetylcholine-evoked chloride currents inXenopus laevis oocytes. J Physiol 366:299–313Google Scholar
  12. Den Hertog A (1982) Calcium and the action of adrenaline, ATP and carbachol on guinea-pig taenia caeci. J Physiol 325: 423–439Google Scholar
  13. Dumont JN (1972) Oogenesis inXenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J Morphol 136:153–180Google Scholar
  14. Ferrero JD, Frischknecht R (1983) Different effector mechanisms for ATP and adenosine hyperpolarization in the guinea-pigTaenia coli. Eur J Pharmacol 87:151–154Google Scholar
  15. Gallacher DV (1982) Are there purinergic receptors on parotid acinar cells? Nature 296:83–86Google Scholar
  16. Goto M, Yatani A, Tsuda Y (1977) An analysis of the action of ATP and related compounds on membrane current tension components in bullfrog atrial muscle. Jpn J Physiol 27:81–94Google Scholar
  17. Gundersen CB, Miledi R, Parker I (1983) Serotonin receptors induced by exogenous messenger RNA inXenopus oocytes. Proc R Soc Lond (Biol) 219:103–109Google Scholar
  18. Hille B (1984) Ionic channels, of exitable membranes. Sinauer, MA, USAGoogle Scholar
  19. Hutter OF, Rankin AC (1982) Increase by adenosine and adenine nucleotides in potassium permeability of sinus venosus of tortoise heart. J Physiol 329:57p-58pGoogle Scholar
  20. Jager JP (1974) The effect of catecholamines and ATP on the smooth muscle cell membrane of the guinea-pig taenia coli. Eur J Pharmacol 25:372–382Google Scholar
  21. Kusano K, Miledi R, Stinnakre, J (1977) Acetylcholine receptors in the oocyte membrane. Nature 270:739–741Google Scholar
  22. Kusano K, Miledi R, Stinnakre J (1982) Cholinergic and catecholaminergic receptors in theXenopus oocytes membrane. J Physiol 328:143–170Google Scholar
  23. Lotan I, Dascal N, Cohen S, Lass Y (1982) Adenosine-induced slow ionic currents inXenopus oocytes. Nature 298:572–574Google Scholar
  24. Lotan I, Dascal N, Oron Y, Cohen S, Lass Y (1985) Adenosineinduced K+ current inXenopus oocytes and the role of adenosine 3′, 5′-monophosphate. Mol Pharmacol 28:170–177Google Scholar
  25. Meszaros J, Kelemen K, Kecskmeti V, Szegi J (1984) Antagonism between adenosine and bromobenzyl-methyl-adamantylamine, a K+ channel blocker in atrial myocardium of guinea pig. Eur J Pharmacol 98:265–268Google Scholar
  26. Miledi R (1982) A calcium-dependent transient outward current inXenopus laevis oocytes. Proc R Soc Lond (Biol) 215:491–497Google Scholar
  27. Miledi R, Parker I (1984) Chloride current induced, by injection of calcium intoXenopus oocytes. J Physiol 357:173–183Google Scholar
  28. Miledi R, Parker I, Sumikawa K (1982) Synthesis of chick brain GABA receptors by frog oocytes. Proc R Soc Lond (Biol) 216:509–515Google Scholar
  29. Mishina M, Kurosaki T, Tobimatsu T, Morimoto Y, Noda M, Yamamoto T, Terao M, Lindsrom J, Takahashi T, Kuno M, Numa S (1984) Expression of functional acetylcholine receptors from cloned cDNAs. Nature 307:604–608Google Scholar
  30. Okada Y, Yada T, Ohno-Shosaku T, Oiki S, Ueda S, Machida K (1984) Exogenous ATP induces electrical membrane responses in fibroblasts. Exp Cell Res 152:552–557Google Scholar
  31. Stone TW (1981) Differential blockade of ATP, noradrenaline and electrically-evoked constructions of the rat vas deferens by nifedipine. Eur J Pharmacol 74:373–376Google Scholar
  32. Tomita T, Watanabe H (1973) A comparison of the effects of adenosine triphosphate with noradrenaline and with the inhibitory potential of the guinea-pigTaenia coli. J Physiol 231:167–177Google Scholar
  33. Yatani A, Goto M, Tsuda Y (1978) Nature of catecholamine-like actions of ATP and other energy rich nucleotides on the bullfrog atrial muscle. Jpn J Physiol 28:47–61Google Scholar
  34. Yatani A, Tsuda Y, Brown AM (1982) Nanomolar concentrations of extracellular ATP activate membrane Ca2+ channel in snail neurons. Nature 296:169–171Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Ilana Lotan
    • 1
  • Nathan Dascal
    • 1
  • Sasson Cohen
    • 1
  • Yoram Lass
    • 1
  1. 1.Department of Physiology and Pharmacology, Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael

Personalised recommendations