Pflügers Archiv

, Volume 337, Issue 4, pp 333–350 | Cite as

The action of lanthanum and D600 on the calcium exchange in the smooth muscle cells of the guinea-pig taenia coli

  • C. J. Mayer
  • C. van Breemen
  • R. Casteels


The calcium distribution in smooth muscle cells of the taenia coli has been studied with a method based on the action of lanthanum ions. La3+-ions make it possible to separate the extracellular calcium from the cellular calcium by their high affinity for extracellular calcium binding sites and by their blocking action on the transmembrane calcium movements. Using this method the cellular calcium content and its exchangeable fraction have been determined. In spontaneously active tissues the exchange of the cellular calcium reaches a maximal value of 108 μM/kg wet wt or 1/6 of the total cellular calcium, while in quiescent tissues (treated with D600) this maximal value is 80 μM/kg wet wt.

During K depolarization a large amount of extracellular calcium penetrates in the cellular compartment. This entry and the corresponding tension development are inhibited by D600.

These results are interpreted by assuming that even during spike discharge the Ca-permeability of the membrane is very small and that this entering calcium could function as a trigger, releasing intracellularly localized calcium. This physiological mechanism should be distinguished from the important uptake of calcium occurring during K-depolarization.

Key words

Smooth Muscle Calcium Distribution Lanthanum D600 Excitation-Concentration Coupling 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anderson, N. C., Ramon, F., Snyder, A.: Studies on calcium and sodium in uterine smooth muscle excitation under current-clamp and voltage-clamp conditions. J. gen. Physiol.58, 322–339 (1971).Google Scholar
  2. Atkins, G. L.: Multicompartment models for biological systems. London: Methuen 1969.Google Scholar
  3. Bauer, H., Goodford, P. J., Hüter, J.: The calcium content and45calcium uptake of the smooth muscle of the guinea-pig taenia coli. J. Physiol. (Lond.)176, 163–179 (1965).Google Scholar
  4. Breemen, C. van: Blockade of membrane calcium fluxes by lanthanum in relation to vascular smooth muscle contractility. Arch. intern. Physiol. Biochem.77, 710–716 (1969).Google Scholar
  5. Breemen, C. van, De Weer, P.: Lanthanum inhibition of45Ca efflux from the squid giant axon. Nature (Lond.)226, 760–761 (1970).Google Scholar
  6. Breemen, C. van, Farinas, B. R., Casteels, R., Gerba, P., Wuytack, F., Deth, R.: Factors controlling cytoplasmic Ca2+ concentration. Phil. Trans. R. Soc. (Lond.) B (in press).Google Scholar
  7. Breemen, C. van, Farinas, B. R., Gerba, P., Mc Naughton, E. D.: Excitation-contraction coupling in rabbit aorta studied by the lanthanum method for measuring cellular calcium influx. Circulat. Res.30, 44–54 (1972).Google Scholar
  8. Breemen, D. van, Breemen, C. van: Calcium exchange diffusion in a porous phospholipid ion-exchange membrane. Nature (Lond.)223, 898–900 (1969).Google Scholar
  9. Bülbring, E., Tomita, T.: Effect of calcium, barium and manganese on the action of adrenaline in the smooth muscle of the guinea-pig taenia coli. Proc. roy. Soc. B172, 121–136 (1969).Google Scholar
  10. Casteels, R., Breemen, C. van, Mayer, C. J.: A study of the calcium distribution in smooth muscle cells of the guinea-pig taenia coli using La3+. Arch. int. Pharmacodyn.199, 193–194 (1972).Google Scholar
  11. Casteels, R., Breemen, C. van, Wuytack, F.: Effect of metabolic depletion on the membrane permeability of smooth muscle cells and its modification by La3+. Nature New Biology239, 249–251 (1972).Google Scholar
  12. Endo, M., Tanaka, M., Ogawa, Y.: Calcium induced release of calcium from the sarcoplasmic reticulum of skinned muscle fibres. Nature (Lond.)228, 34–36 (1970).Google Scholar
  13. Fleckenstein, A., Grün, G., Tritthart, H., Byon, K.: Uterus-Relaxation durch hochaktive Ca++-antagonistische Hemmstoffe der elektro-mechanischen Koppelung wie Isoptin (Verapamil, Iproveratril), Substanz D600 und Segontin (Prenylanin). Klin. Wschr.49, 32–41 (1971).Google Scholar
  14. Golenhofen, K., Lammel, E.: Selective suppression of some components of spontaneous activity in various types of smooth muscle by iproveratril (verapamil). Pflügers Arch.331, 233–243 (1972).Google Scholar
  15. Goodford, P. J.: The loss of radioactive45Calcium from the smooth muscle of the guinea-pig taenia coli. J. Physiol. (Lond.)176, 180–190 (1965).Google Scholar
  16. Lammel, E., Golenhofen, K.: Messungen der45Ca-Aufnahme an intestinaler glatter Muskulatur zur Hypothese einer von Ca-Ionen getragenen Aktionsstromes. Pflügers Arch.329, 269–282 (1971b).Google Scholar
  17. Lammel, E., Golenhofen, K.: Erregbarkeit und Ca-Austausch intestinaler glatter Muskel (Taenia coli des Meerschweinchens) unterhalb der kritischen Temperatur für die Spontanaktivität. Pflügers Arch.329, 258–268 (1971a).Google Scholar
  18. Lettvin, J. Y., Pickard, W. F., Mc Cullogh, W. S., Pitts, W.: A theory of passive ion flux through axon membranes. Nature (Lond.)202, 1338–1339 (1964).Google Scholar
  19. Lüllmann, H.: Calcium fluxes and calcium distribution in smooth muscle. In: Smooth muscle. Ed. by E. Bülbring, A. Brading, A. Jones, and T. Tomita. London: Edw. Arnold 1970.Google Scholar
  20. Mela, L.: Interaction of La3+ and local anesthetic drugs with mitochondrial Ca++ and Mn++ uptake. Arch. Biochem.123, 286–293 (1968).Google Scholar
  21. Patterson, M. S., Greene, R. C.: Measurement of low-energy beta-emitters in aqueous solution by liquid scintillation counting og emulsions. Analyt. Chem.37, 854–857 (1965).Google Scholar
  22. Reuter, H., Blaustein, M. P., Haeusler, G.: Na-Ca exchange and tension development in arterial smooth muscle. Phil. Trans. R. Soc. (Lond.) B (in press).Google Scholar
  23. Schatzmann, H. J.: Calciumaufnahme und Abgabe am Darmmuskel des Meerschweinchens. Pflügers Arch. ges. Physiol.274, 295–310 (1961).Google Scholar
  24. Schatzmann, H. J., Tschabold, M.: The Lanthanides Ho3+ and Pr3+ as inhibitors of calcium transport in human red cells. Experientia (Basel)27, 59–61 (1971).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • C. J. Mayer
    • 1
  • C. van Breemen
    • 1
  • R. Casteels
    • 1
  1. 1.Laboratorium voor FysiologieUniversiteit LeuvenBelgium

Personalised recommendations