Pflügers Archiv

, Volume 323, Issue 2, pp 121–140 | Cite as

Lipid soluble weak organic acid buffers as “substrate” for pancreatic secretion

  • I. Schulz
  • F. Ströver
  • K. J. Ullrich


The isolated cat pancreas was perfused with a Krebs-Henseleitsolution containing different concentrations of sulfamerazine buffer. Concomitantly the secretory rate, pH value, Cl and total buffer concentration in the secretion were measured. The following were found:
  1. 1.

    The secretory rate dropped to very small values when the bicarbonate buffer was omitted from the perfusate.

  2. 2.

    Sulfamerazine can partly replace the CO2-bicarbonate buffer in promoting water and solute secretion in the saline-perfused preparation of the cat's pancreas.

  3. 3.

    The secretion of the sulfamerazine buffer depended exclusively on the concentration of its undissociated component in the perfusate.


It is concluded that a separation of H+/OH ions takes place presumably at the luminal cell border and that the undissociated form of sulfamerazine can penetrate the cell by ‘nonionic diffusion’. In this way a source of H+ ions is provided which may be used either for H+ transport or for the buffering of OH ions. The process of nonionic diffusion seems to be rate limiting for the buffer secretion, at least when sulfamerazine is offered.


Pancreas Bicarbonate Secretion Buffer Secretion H+ Ion Transport Weak Organic Acids 


Pankreas Bicarbonatsekretion Puffersekretion H+-Ionentransport schwache organische Säuren 


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Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • I. Schulz
    • 1
  • F. Ströver
    • 1
  • K. J. Ullrich
    • 1
  1. 1.Max-Planck-Institut für BiophysikFrankfurt a.M.Germany

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