Secretory transmembrane potentials in acinar cells from the cat submandibular gland during perfusion with a chloride-free sucrose solution
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The cat submandibular gland was perfused with a normal NaCl Locke solution and a chloride-free sucrose solution. The numerical increase in acinar membrane potential (secretory potential) was recorded after intra-arterial injection of acetylcholine.
There was no significant difference between the size of the secretory potentials recorded during perfusion with the sucrose solution [23.6 mV±1.4 (n=23)] and the size of those recorded during the control periods [20.6 mV±1.2 (n=24)].
The maximal value of the membrane potential after injection of acetylcholine was higher [51.8 mV±2.4 (n=23)] during perfusion with the sucrose solution than during the control periods [44.8 mV±1.8 (n=22)].
The results show that a pump transporting chloride into the acinar cells cannot be responsible for the generation of the secretory potentials. The results are best accounted for by assuming that an outward passive transport of potassium, being partly short-circuited by an inward passive sodium transport, is responsible for the change in membrane potential after stimulation with acetylcholine.
Key-WordsSecretory Potential Salivary Gland Chloride Pump Sucrose Potassium Transport
SchlüsselwörterSekretorisches Potential Speicheldrüse Chlorid-Pumpe Sucrose Kaliumtransport
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- 1.Burgen, A. S. V.: Membrane potentials and the secretory activity of the acinar cells. In: Secretory Mechanisms of Salivary Glands, pp. 3–10. Eds.: L. H. Schneyer and C. A. Schneyer. New York-London: Acad. Press Inc. 1967.Google Scholar
- 2.Davenport, H. W.: Physiology of the Digestive Tract, p. 88. Chicago: Year book medical publishers Inc. 1966.Google Scholar
- 3.Lundberg, A.: Electrophysiology of salivary glands. Physiol. Rev.38, 21–39 (1958).Google Scholar
- 4.Petersen, O. H.: Some factors influencing stimulation-induced release of potassium from the cat submandibular gland to fluid perfused through the gland. J. Physiol. (Lond.)208, 431–447 (1970).Google Scholar
- 5.—: The dependence of the transmembrane salivary secretory potential on the external potassium and sodium concentration. J. Physiol. (Lond.)210, 205–215 (1970).Google Scholar
- 6.— Poulsen, J. H.: Secretory potentials, potassium transport and secretion in the cat submandibular gland during perfusion with sulphate Locke's solution. Experientia (Basel)24, 919–920 (1968).Google Scholar
- 7.Petersen, O. H., Poulsen, J. H.: Secretory transmembrane potentials and electrolyte transients in salivary glands. In: Exocrine Glands, pp. 3–20. Eds. S. Y. Botelho, F. P. Brooks and W. B. Shelley. Philadelphia: University of Pennsylvania Press 1969.Google Scholar
- 8.——, Thorn, N. A.: Secretory potentials, secretory rate and water permeability of the duct system in the cat submandibular gland during perfusion with calcium-free Locke's solution. Acta physiol. scand.71, 203–210 (1967).Google Scholar
- 9.———: The importance of calcium ions for the transport of electrolytes and water in salivary glands. Proc. Int. Union Physiol. Sci.7, 345 (1968).Google Scholar
- 10.Ussing, H. H., Kruhøffer, P., Thaysen, J. H., Thorn, N. A.: The alkali metal ions in biology. Handbuch der experimentellen Pharmakologie. Ergänzungswerk Bd. 13, S. 113, 429. Berlin-Göttingen-Heidelberg: Springer 1960.Google Scholar