Pflügers Archiv

, Volume 345, Issue 3, pp 221–236 | Cite as

Die Wirkung aktiv transportierter Zucker auf den Natrium-, Kalium- und Volumentransport am Jejunum und Ileum der Ratte

  • R. Dennhardt
  • F. J. Haberich
Article

The action of actively transported sugars on the sodium, potassium, and fluid transport in the jejunum and ileum of the rat

Summary

Perfusion experiments were carried out in the proximal jejunum and in the distal ileum of conscious rats, using a newly devised technique. The stimulation effect of actively transported hexoses on the sodium, potassium and fluid absorption is demonstrated.
  1. 1.

    There are quantitative differences concerning the effect of 3-O-methyl-d-glucose andd-glucose on the sodium and water absorption.

     
  2. 2.

    The stimulation by glucose can be seen only when the glucose concentration in the luminal fluid is greater than 10 mM.

     
  3. 3.

    The ratio of 3-O-methyl-d-glucose- to sodium-transfer-rate is >1, while it is exactly 1 in the experiments involvingd-glucose.

     
  4. 4.

    The sodium-water equivalent is isotonic under all experimental conditions.

     
  5. 5.

    In the presence of actively transported hexoses the secretion of potassium into an initially K+-free perfusate is significantly diminished.

     
  6. 6.

    The transport of potassium depends on the net fluid movement.

     
  7. 7.

    The stimulation of glucose and 3-O-methyl-d-glucose can only be demonstrated in the jejunum of rat whereas no effect is seen in the distal ileum and colon.

     
  8. 8.

    These results suggest that “solvent drag” isone factor which under the above mentioned experimental conditions affects the enhanced electrolyte and water absorption in the jejunum of the rat.

     

Key words

Intestinal Water and Electrolyte Absorption Effect of Actively Transported Hexoses Conscious Rats 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Barry, R. J. C., Eggenton, J., Smyth, D. H.: Sodium pumps in the rat small intestine in relation to hexose transfer and metabolism. J. Physiol. (Lond.)204, 299–310 (1969)Google Scholar
  2. Barry, R. J. C., Smyth, D. H., Wright, E. M.: Short circuit current and solute transfer by rat jejunum. J. Physiol. (Lond.)181, 410–431 (1965)Google Scholar
  3. Barry, B. A., Matthews, J., Smyth, D. H.: Transfer of glucose and fluid by different parts of the small intestine of the rat. J. Physiol. (Lond.)157, 279–288 (1961)Google Scholar
  4. Clarkson, T. W., Rothstein, A.: Transport of monovalent cations by the isolated small intestine of the rat. Amer. J. Physiol.199, 898–906 (1960)Google Scholar
  5. Code, D. F., Philipps, S. F., Swallow, J. H.: The final common sorption fluids of small and large bowel. J. Physiol. (Lond.)187, 42–43P (1966)Google Scholar
  6. Crane, R. K.: Intestinal absorption of sugars. Physiol. Rev.40, 789–825 (1965)Google Scholar
  7. Crane, R. K.: Sodium dependent transport in the intestine and other animal tissues. Fed. Proc.24, 1000–1006 (1965)Google Scholar
  8. Crane, R. K., Miller, D., Bihler, L.: The restrictions on possible mechanisms of intestinal active transport of sugars. In: A. Kleinzeller and A. Kotyk (Ed.): Membrane transport and metabolism. New York: Academic Press 1961Google Scholar
  9. Cummins, A. J., Jussila, R.: Comparison of gluose absorption rates in the upper and lower human small intestine. Gastroenterology29, 982 (1955)Google Scholar
  10. Curran, P. F.: Ion transport in intestine and its coupling to other transport processes. Fed. Proc.24, 993–999 (1965)Google Scholar
  11. Fisher, R. B., Parsons, D. S.: Glucose absorption from surviving rat small intestine. J. Physiol. (Lond.)110, 281 (1950)Google Scholar
  12. Fisher, R. B., Parsons, D. S.: Glucose movement across the wall of the rat small intestine. J. Physiol. (Lond.)119, 210–223 (1953)Google Scholar
  13. Fordtran, J. S., Rector, F. C., Jr., Carter, N. W.: The mechanisms of sodium absorption in the human small intestine. J. clin. Invest.47, 884–900 (1968)Google Scholar
  14. Fordtran, J. S., Rector, F. C., Jr., Ewton, M. F., Soter, N., Kinney, J.: Permeability characteristics of the human small intestine. J. clin. Invest.44, 1935 (1965)Google Scholar
  15. Fordtran, J. S., Rector, F. C., Jr., Locklear, T. W., Ewton, M. F.: Water and solute movement in the small intestine of patients with sprue. J. clin. Invest.46, 287 (1967)Google Scholar
  16. Fullerton, P. M., Parsons, D. S.: The absorption of sugars and water from rat intestine in vivo. Quart. J. exp. Physiol.41, 387–397 (1956)Google Scholar
  17. Gilman, A., Koelle, E., Ritchie, J. M.: Transport of potassium ions in the rat's intestine. Nature (Lond.)197, 1210–1211 (1963)Google Scholar
  18. Goldner, A. M., Schultz, S. G., Curran, P. F.: Sodium and sugar fluxes across the mucosal border of rabbit ileum. J. gen. Physiol.53, 362–383 (1969)Google Scholar
  19. Haberich, F. J., Aziz, O., Herzer, R., Dennhardt, R.: Resorptions- und Sekretionsstudien am Darm. I. Mitteilung: Technik der extrakorporalen Perfusion beliebiger, vorübergehend funktionell isolierter Darmabschnitte an der wachen Ratte. Z. ges. exp. Med.148, 223–237 (1968)Google Scholar
  20. Hoshiko, T., Lindley, B. D.: The relationship of Ussing's flux-ratio to the thermodynamic description of membrane permeability. Biochim. biophys. Acta (Amst.)79, 301–317 (1964)Google Scholar
  21. Kedem, O., Katchalsky, A.: Thermodynamic analysis of the permeability of biological membranes to non-electrolytes. Biochim. biophys. Acta (Amst.)27, 229–246 (1958)Google Scholar
  22. Levinson, R. A., Schedl, H. P.: Absorption of sodium, chloride, water, and simple sugars in rat small intestine. Amer. J. Physiol.211, 939–942 (1966)Google Scholar
  23. Magee, H. E., Reid, E.: Absorption of glucose from the alimentary canal. J. Physiol. (Lond.)73, 163 (1931)Google Scholar
  24. Malawer, S. J., Ewton, M. F., Fordtran, J. S., Ingelfinger, F. J.: Interrelation between jejunal absorption of sodium, glucose, and water in man. J. clin. Invest..44, 1072–1073 (1965)Google Scholar
  25. Schedl, H. P., Clifton, J. A.: Solute and water absorption by the human small intestine. Nature (Lond.)199, 1264–1267 (1963)Google Scholar
  26. Schultz, S. G., Curran, P. F.: Stimulation of intestinal sodium absorption by sugars. J. clin. Nutr.23, 437–440 (1970)Google Scholar
  27. Schultz, S. G., Zalusky, R.: Ion transport in isolated rabbit ileum. II. The interaction between active sodium and active sugar transport. J. gen. Physiol.47, 1043–1059 (1964)Google Scholar
  28. Schultz, S. G., Zalusky, R.: Interactions between active sodium transport and active amino-acid transport in isolated rabbit ileum. Nature (Lond.)205, 292–294 (1965)Google Scholar
  29. Sladen, G. E.: A review of water and electrolyte transport. In: W. L. Burland and P. D. Samuel (Ed.): Transport across the intestine, pp. 14–34, Edinburgh-London: Churchill Livingstone 1972Google Scholar
  30. Sladen, G. E., Dawson, A. M.: Interrelationships between the absorptions of glucose, sodium, and water by the normal human jejunum. Clin. Sci.36, 119 to 132 (1969)Google Scholar
  31. Taylor, A. E., Wright, E. G., Schultz, S. G., Curran, P. F.: Effect of sugars on ion fluxes on intestine. Amer. J. Physiol.214, 836–842 (1968)Google Scholar
  32. Turnberg, L. A.: Potassium transport in the human small bowel. Gut12, 811–818 (1971)Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • R. Dennhardt
    • 1
  • F. J. Haberich
    • 1
  1. 1.Institut für Angewandte Physiologie der Philipps-Universität MarburgMarburg (Lahnberge)Germany

Personalised recommendations