Pflügers Archiv

, Volume 357, Issue 3–4, pp 291–301 | Cite as

Glomerular filtration rate and microsphere distributions in single nephron of rat kidney

  • P. Poujeol
  • D. Chabardès
  • J. P. Bonvalet
  • C. de Rouffignac
  • S. Deiss


In control non diuretic (ND) and in salt-loaded (SL) rats, both the microsphere technique and the14C ferrocyanide infusion technique were used to determine the distribution of microspheres in single glomeruli and the SNGFR of the corresponding nephrons. A sample of microspheres with a diameter averaging 11.0±2.6 μ SD was selected from a 15±5 μ unlabelled batch. In each rat, three million of these microspheres were injected through the left carotid artery. The microspheres were directly counted under microscopic observation in the glomeruli of the nephrons which were microdissected to determine the SNGFR value. The number of microspheres per glomerulus for a given kidney generally varied from 0 to 8 and was independant to the SNGFR value. The diameter of the microspheres trapped was constant in all the animals. The mean number of microspheres for superficial (S) and juxtamedullary (JM) nephrons was, (ND):S=1.99±0.48 SE.,n=5; JM=3.02±0.51 SE,n=5;P<0.02, (SL):S=3.75±0.53 SE,n=6; JM=2.86±0.33 SE,n=6;P<0.05. This distribution was directly related to that of SNGFR in ND rats (S=39.0±6.1 SD and JM=49.5±10.3 nl/min) but not in SL rats (S=50.9±6.1 and JM=66.9±10.0 nl/min). In conclusion, the microsphere technique described in the present paper, appears more suitable for investigating single glomerular blood flow since the number and the size of the microspheres trapped are directly determined at the level of the glomerulus.

Key words

Single Nephron Glomerular Filtration Rate Microsphere Distribution Microdissection Extra Cellular Fluid Volume Expansion 


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  1. 1.
    Bankir, L., Farman, N. (avec la collaboration technique de Huet de la Tour, E.): Hétérogénéité des glomérules chez le lapin. Arch. Anat. micr. Morph. exp.62, 281–291 (1973)Google Scholar
  2. 2.
    Bankir, L., Farman, N., Huet de la Tour, E., Grunfeld, J. P.: Radioactive microsphere distribution and single glomerular blood flow in the normal rabbit kidney. Pflügers Arch.342, 111 (1973)Google Scholar
  3. 3.
    Barrat, L. J., Wallin, J. D., Rector, F. C., Seldin, D. W.: Influence of volume expansion on single nephron filtration rate and plasma flow in the rat. Amer. J. Physiol.224, 643–650 (1973)Google Scholar
  4. 4.
    Bray, G. A.: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyst. Biochem.1, 279–285 (1960)Google Scholar
  5. 5.
    Brenner, B. M., Troy, J. L., Daugharty, T. M., Deen, W. M.: Dynamics of glomerular ultrafiltration in the rat. II. Plasma flow dependence of GFR. Amer. J. Physiol.223, 1184–1190 (1972)Google Scholar
  6. 6.
    Chabardès, D., Poujeol, P., Deiss, S., Bonvalet, J. P., Rouffignac, C. de: Intrarenal glomerular filtration rate distribution in salt-loaded rats. Pflügers Arch.349, 191–202 (1974)Google Scholar
  7. 7.
    Coehlo, J. B., Chien, K. C. H., Bradley, S. E.: Measurement of single nephron glomerular filtration rate without micropuncture. Amer. J. Physiol.223, 832–839 (1972)Google Scholar
  8. 8.
    Coehlo, J. B., Bradley, S. E.: Role of a redistribution of single glomerular filtration rate (SNGFR) in sodium excretion. 5th Inter. Congr. Nephrol. Mexico (Abstr.), p. 89 (1972)Google Scholar
  9. 9.
    Hanssen, O. E.: Method for comparison of glomerular filtration in individual rat nephrons. 2nd Intr. Congr. Nephrol. Prague (Abstr.), p. 523 (1963)Google Scholar
  10. 10.
    Kallskog, O., Ulfendahl, H. R., and Wollgast, M.: Single glomerular blood flow as measured with carbonized 141-Ce-labelled microspheres. Acta physiol. scand.85, 408–413 (1972)Google Scholar
  11. 11.
    Katz, M. A., Blantz, R. C., Rector, F. C., Seldin, D. W.: Measurement of intrarenal blood flow. I. Analysis of microsphere method. Amer. J. Physiol.220, 1903–1913 (1971)Google Scholar
  12. 12.
    Lassiter, W. E., Mylle, M., Gottschalk, C. W.: Net transtubular movement of water and urea in saline diuresis. Amer. J. Physiol.206, 669–673 (1964)Google Scholar
  13. 13.
    McNay, J. L., Abe, Y.: Pressure dependant heterogeneity of renal cortical blood flow in dog. Circulat. Res.27, 571–587 (1970)Google Scholar
  14. 14.
    Poujeol, P., Chabardès, D., Bonvalet, J. P., Rouffignac, C. de: Microsphères: nouvelle technique d'utilisation pour l'étude de la distribution intracorticale du flux sanguin rénal. Résultats chez le rat normal et surchargé en sodium. J. Physiol. (Paris)65, 481 (1972)Google Scholar
  15. 15.
    Rouffignac, C. de, Deiss, S., Bonvalet, J. P.: Détermination du taux individuel de filtration glomérulaire des néphrons accessibles et inaccessibles à la microponction. Pflügers Arch.315, 273–290 (1970)Google Scholar
  16. 16.
    Rouffignac, C. de, Morel, F., (avec la collaboration technique de Deiss, S.): Etude par microdissection de la distribution et de la longueur des tubules proximaux dans le rein de cinq espèces de rongeurs. Arch. Anat. micr. Morph. exp.56, 123–132 (1968)Google Scholar
  17. 17.
    Rudolf, A. M., Heymann, M. A.: Measurement of flow in perfused organs, using microsphere techniques. Karolinska symposia on research methods in reproductive endocrinology. 4th Symposium Perfusion techniques, pp. 112–127 (1971)Google Scholar
  18. 18.
    Sapirstein, L. A.: Regional blood flow by fractional distribution of indicators. Amer. J. Physiol.193, 161–168 (1958)Google Scholar
  19. 19.
    Schnermann, J., Wright, F. S., Davis, J. M., Stackelberg, W. V., Grill, G.: Regulation of superficial nephron filtration rate by tubulo-glomerular feedback. Pflügers Arch.318, 147–175 (1970)Google Scholar
  20. 20.
    Wallin, J. D., Blantz, R. C., Katz, M. A., Andeucci, W. E., Rector, F. C., Seldin, D. W.: Effect of saline diuresis on intrarenal blood flow in the rat. Amer. J. Physiol.221, 1297–1304 (1971)Google Scholar
  21. 21.
    Wallin, J. D., Rector, F. C., Seldin, D. W.: Measurement of intrarenal plasma flow with antiglomerular basement-membrane antibody. Amer. J. Physiol. 1621–1628 (1971)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • P. Poujeol
    • 1
  • D. Chabardès
    • 1
  • J. P. Bonvalet
    • 1
  • C. de Rouffignac
    • 1
  • S. Deiss
    • 1
  1. 1.Département de BiologieC.E.N. SaclayGif-sur-YvetteFrance

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