Summary
AVP dependent adenylate cyclase activity was measured in single pieces of 8 different tubular segments isolated from collagenase treated rabbit kidneys.
High responses were observed in all the tested portions of the collecting tubule, that is its cortical branched part (BCT), its cortical straight part (CCT) and its outer medullary part (MCT). Dose response curves indicated in CCT: 2 fold threshold stimulation at 10−11 M AVP, 27 fold stimulation at 10−6 M AVP, half maximal stimulation at about 10−9 M AVP.
Both the medullary (MAL) and, to a lesser exten, the cortical (CAL) portions of the thick ascending limb were also observed to contain AVP sensitive adenylate cyclase (for MAL: 2 fold threshold stimulation at 10−9 M AVP, 9 fold stimulation at 10−7 M AVP, half maximal stimulation at 5×10−9 M AVP).
In contrast, nearly no responsiveness to AVP was observed in the proximal convoluted tubule, in the thin descending limb of the loop and in the distal convoluted tubule (DCT).
The limited response obtained in DCT (which is a structure generally considered as a target site for AVP) as well as the clearcut effect elicited by AVP in MAL (the functioning of which is not known to be controlled by ADH) were unexpected observations; their possible physiological implications will be discussed.
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Antoniou, L. D., Burke, T. J., Robinson, R. R., Clapp, R. J.: Vasopressin related alterations of sodium reabsorption in the loop of Henle. Kidney Internat.3, 6–13 (1973)
Atherton, J. C., Green, R., Thomas, S.: Influence of lysine-vasopressin dosage on the time course of changes in renal tissue and urinary composition in the conscious rat. J. Physiol. (Lond.)213, 291–309 (1971)
Bastide, F., Jard, S.: Action of noradrenalin and oxytocin on the active transport of sodium and the permeability of frog skin to water. Role of cyclic AMP. Biochim. biophys. Acta (Amst.)150, 113–123 (1968)
Beck, N. P., Kaneko, T., Zor, U., Field, J. B., Davis, B. B.: Effects of vasopressin and prostaglandin E1 on the adenyl cyclase-cyclic 3′,5′-adenosine monophosphate system of the renal medulla of the rat. J. clin. Invest.50, 2461–2465 (1971)
Bennett, C. M., Brenner, B. M., Berliner, R. W.: Micropuncture study of nephron function in the rhesus monkey. J. clin. Invest.47, 203–216 (1968)
Bockaert, J., Roy, C., Jard S.: Oxytocin-sensitive adenylate cyclase in frog bladder epithelial cells. J. biol. Chem.247, 7073–7081 (1972)
Bockaert, J., Roy, C., Rajerison, R., Jard, S.: Specific binding of3H lysinevasopressin to pig kidney plasma membranes. J. biol. Chem.248, 5922–5931 (1973)
Bray, G. A.: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem.1, 279–285 (1960)
Burg, M. B., Green, N.: Function of the thick ascending limb of Henle's loop. Amer, J. Physiol.224, 659–668 (1973)
Chabardès, D., Imbert, M., Clique, A., Montegut, M., Morel, F.: PTH sensitive adenyl cyclase activity in different segments of the rabbit nephron. Pflügers Arch.354, 229–239 (1975)
Chase, L. R., Aurbach, G. D.: Renal adenyl cyclase: anatomically separate sites for parathyroid hormone and vasopressin. Science159, 545–546 (1968)
Clapp, J. R., Robinson, R. R.: Osmolality of distal tubular fluid in the dog. J. clin. Invest.45 1847–1853 (1966)
Davis, B. B., Knox, F. G., Berliner, R. W.: Effect of vasopressin on proximal tubule sodium reabsorption in the dog. Amer. J. Physiol.212, 1361–1364 (1967)
Dousa, T., Hechter, O., Schwartz, I. L., Walter, R.: Neurohypophyseal hormone-responsive adenylate cyclase from mammalian kidney. Proc. nat. Acad. Sci. (Wash.)68, 1693–1697 (1971)
Forte, L.: Characterization of the adenyl cyclase of rat kidney plasma membranes. Biochim. biophys. Acta (Amst.)266, 524–542 (1972)
Ganote, C. E., Grantham, J. J., Moses, H. L., Burg, M. B., Orloff, J.: Ultrastructural studies of vasopressin effect on isolated perfused renal collecting tubules of the rabbit. J. Cell biol.36, 355–367 (1968)
Gottschalk, C. W.: Micropuncture studies of tubular function in the mammalian kidney. Physiologist4, 35–55 (1961)
Gottschalk, C. W., Mylle, M.: Micropuncture study of the mammalian urinary concentrating mechanism: evidence for the countercurrent hypothesis. Amer. J. Physiol.196, 927–936 (1959)
Grantham, J. J., Burg, M.: Effect of vasopressin and cyclic AMP on permeability of isolated collecting tubules. Amer. J. Physiol.211, 255–259 (1966)
Grantham, J. J., Orloff, J.: Effect of prostaglandin E1 on the permeability response of the isolated collecting tubule to vasopressin, adenosine 3′5′-monophosphate and theophylline. J. clin. Invest.47, 1154–1161 (1968)
Handler, J. S., Butcher, R. W., Sutherland, E. W., Orloff, J.: The effect of vasopressin and of theopylline on the concentration of adenosine 3′5′-phosphate in the urinary bladder of the toad. J. biol. Chem.240, 4524–4526 (1965)
Imbert, M., Chabardès, D., Montegut, M., Clique, A., Morel, F.: Adenylate cyclase activity along the rabbit nephron as measured in single isolated segments. Pflügers Arch.354, 213–228 (1975)
Lechene, C., Morel, F., Guinnebault, M., De Rouffignac, C.: Etude par microponction de l'élaboration de l'urine. I. Chez le rat dans differents états de diurèse. Nephron6, 457–477 (1969)
Martinez-Maldonado, M., Eknoyan, G., Suki, W. N.: Natriuretic effects of vasopressin and cyclic AMP; possible site of action in the nephron. Amer. J. Physiol.220, 2013–2020 (1971)
Morel, F.: Action of neurohypophyseal hormones on the active transport of sodium. Reprinted from J. De Graeff and B. Leijnse (Editors). Water and electrolyte metabolism (II), pp. 91–103. Amsterdam: Elvesier Publ. Comp. 1964
Morel, F., De Rouffignac, C., Marsh, D., Guinnebault, M., Lechene, C.: Etude par microponction de l'élaboration de l'urine. II. Chez le Psammomys non diuretique. Nephron6, 553–570 (1969)
Morgan, T. F., Sakai, F., Berliner, R. W.: In vitro permeability of medullary collecting ducts to water and urea. Amer. J. Physiol.214, 574–581 (1968)
Neer, E. J.: The vasopressin-sensitive adenylate cyclase of the rat renal medulla. J. biol. Chem.248, 4775–5781 (1973)
Pruzik, Z., Sedlákova, E., Barth, T.: Isolation of [Arg 8]-vasopressin from the neurophysin complex by free-flow continuous electrophoresis. Hoppe Seylers Z. physiol. Chem.353, 1837–1844 (1972)
Rajerison, R., Marchetti, J., Roy, C., Bockaert, J., Jard, S.: The vasopressin sensitive adenylate cyclase of the rat kidney: effect of adrenalectomy and corticosteroids on hormonal receptor-enzyme coupling. J. biol. Chem.249, 6390–6400 (1974)
Ramachandran, J., Lee, V.: Divergent effects of 0-nitrophenyl sulfenyl ACTH on rat and rabbit fat cell adenyl cyclase. Biochem. biophys. Res. Commun.41, 358–366 (1970)
Robertson, G. L., Mahr, E. A., Athar, S., Sinha, T.: Development and clinical application of a new method for the radioimmunoassay of arginine-vasopressin in human plasma. J. clin. Invest.52, 2340–2352 (1973)
Rocha, A. S., Kokko, J. P.: Sodium chloride and water transport in the medullar thick ascending limb of Henle. Evidence for active chloride transport. J. clin. Invest.52, 612–623 (1973)
Rouffignac, C. De, Lechene, C., Guinnebault, M., Morel, F.: Etude par microponction de l'élaboration de l'urine. III. Chez le Merion non diurétique et en diurèse par le mannito. Nephron6, 643–666 (1969)
Schnermann, J., Valtin, H., Thurau, K., Nagel, W., Horster, M., Fischbach, H., Wahl, M., Liebau, G.: Micropuncture studies on the influence of antidiuretic hormone on tubular fluid reabsorption in rats with hereditary diabetes insipidus. Pflügers Arch.306, 103–118 (1969)
Tisher, C. C., Bulger, R. E., Valtin, H.: Morphology of the renal medulla in water diuresis and vasopressin induced antidiuresis. Amer. J. physiol.220, 87–94 (1971)
Ullrich, K. J., Rumrich, G., Fuchs, G.: Wasserpermeabilität und transtubulärer Wasserfluß corticaler Nephronabschnitte bei verschiedenen Diuresezuständen. Pflügers Arch. ges. Physiol.280, 99–119 (1964)
Ullrich, K. J., Schmidt-Nielsen, B., O'Dell, R., Pehling, G., Gottschalk, C. W., Lassiter, W. E., Mylle, M.: Micropuncture study of composition of proximal and distal tubular fluid in rat kidney. Amer. J. Physiol.204, 527–531 (1963)
Wen, S. F.: The effect of vasopressin on phosphate transport in the proximal tubule of the dog. J. clin. Invest.53, 660–664 (1974)
wirz, H.: Der osmotische Druck in den corticalen Tubuli der Rattenniere. Helv. physiol. pharmacol. Acta14, 353–362 (1956)
Woodhall, P. B., Tisher, C. C.: Response of the distal tubule and cortical collecting duct to vasopressin in the rat. J. clin. Invest.52, 3095–3108 (1973)
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This work was supported by a grant from the C.N.R.S. to the LRA no. 219.
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Imbert, M., Chabardès, D., Montegut, M. et al. Vasopressin dependent adenylate cyclase in single segments of rabbit kidney tubule. Pflugers Arch. 357, 173–186 (1975). https://doi.org/10.1007/BF00585973
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DOI: https://doi.org/10.1007/BF00585973