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Pflügers Archiv

, Volume 427, Issue 1–2, pp 71–79 | Cite as

Modulation of type A K+ current inDrosophila larval muscle by internal Ca2+; effects of the overexpression of frequenin

  • C. Poulain
  • A. Ferrús
  • A. Mallart
Excitable Tissues and Central Nervous Physiology

Abstract

The calcium-dependent modulation of type A K+ current (IA) has been investigated using a two-electrode voltage clamp on larval muscle cells ofDrosophila. It was found that the amplitude ofIA increases when [Ca2+]0 is changed from 0.2 mM to 2 mM. The increase inIA amplitude is not due to overlap with the Ca2+-dependent fast K+ current,ICF, since it is observed also inslo1 mutants, which are deficient for this current. This effect is not due to Ca2+-dependent shifts in the steady-state activation/inactivation kinetics. The phenomenon is probably due to elevations in internal calcium since it is abolished by Ca2+ channel blockers and promoted by caffeine (5 mM) if added in the absence of external calcium. This calcium effect was dose-dependent since it was not observed in the presence caffeine plus 2 mM calcium in the bath nor for values of [Ca2+]0 above 4 mM. The Ca2+-dependent modulation ofIA is absent inV7, a mutation that causes overexpression of frequenin, a recoverin-like Ca2+-binding protein which stimulates guanylyl cyclase [31]. One possible explanation for the loss ofIA modulation in theV7 mutation is that the excess of frequenin alters intracellular cGMP-dependent metabolic pathways responsible for the internal calcium homeostasis.

Key words

Drosophila melanogaster Muscle A current Calcium Frequenin 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • C. Poulain
    • 1
  • A. Ferrús
    • 1
  • A. Mallart
    • 1
  1. 1.Unité de Physiologie Neuromusculaire, Laboratoire de Neurobiologie Cellulaire et MoléculaireCNRSGif-sur-YvetteFrance

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