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Pflügers Archiv

, Volume 427, Issue 1–2, pp 56–63 | Cite as

A calcium-activated nonselective cationic channel in the basolateral membrane of outer hair cells of the guinea-pig cochlea

  • Thierry Van den Abbeele
  • Patrice Tran Ba Huy
  • Jacques Teulon
Excitable Tissues and Central Nervous Physiology

Abstract

The patch-clamp technique was used to investigate ion channels in the basolateral perilymph-facing membrane of freshly isolated outer hair cells (OHCs) from the guinea-pig cochlea. These sensory cells probably determine, via their motile activity, the fine tuning of sound frequencies and the high sensitivity of the inner ear. A Ca2+-activated nonselective cationic channel was found in excised inside-out membrane patches. The current/voltage relationship was linear with a unit conductance of 26.3±0.3 pS (n=15) under symmetrical inger conditions. The channel excluded anions (PNa/PCl=18 wherePNa/PCl denotes the relative permeability of Na to Cl); it was equally permeant to the Na+ and K+ ions and exhibited a low permeability toN-methyl-D-glucamine and Ba2+ or Ca2+. Channel opening required a free Ca2+ concentration of about 10−6 mol/l on the internal side of the membrane and the open probability (Po) was maximal at 10−3 mol/l (Po=0.72±0.06,n=12). Adenosine 5′mono-, tri- and di-phosphate reducedPo to 29±14 (n=5), 42±10 (n=8) and 51±12 (n=5) % of controlPo, respectively, when they were added at a concentration of 10−3 mol/l to the internal side. The channel was partially blocked by flufenamic acid (10−4 mol/l) and 3′,5′-dichlorodiphenylamine-2-carboxylic acid (DCDPC, 10−5 mol/l). This type of channel, together with Ca2+-activated K+ channels, might participate in the control of membrane potential and modulate the motility of OHCs.

Key words

Inner ear Hair cells Patch-clamp Nonselective cationic channels 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Thierry Van den Abbeele
    • 1
    • 2
  • Patrice Tran Ba Huy
    • 2
  • Jacques Teulon
    • 1
  1. 1.Faculté de médecine Necker-Enfants MaladesINSERM U.323ParisFrance
  2. 2.Faculté de médecine LariboisièreLaboratoire d'otologie expérimentaleParisFrance

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