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Pflügers Archiv

, Volume 427, Issue 1–2, pp 24–32 | Cite as

Intracellular Cl concentration in striated intralobular ducts from rabbit mandibular salivary glands

  • K. R. Lau
  • R. L. Evans
  • R. M. Case
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

Intralobular striated ducts have been isolated from rabbit mandibular salivary glands and maintained in primary culture for up to 2 days. Such ducts were loaded with the Cl-sensitive fluorescent dyeN-(ethoxycarbonylmethyl)-(6-methoxyquinolinium bromide) (MQAE) and intracellular Cl concentration ([Cl]i monitored using a fluorescence microscope. Intracellular Cl could be rapidly and reversibly emptied from striated duct cells by replacing Cl in the superfusing solution with NO 3 . [Cl]i could be lowered by removal of external Na+, exposure to 10 μM amiloride or to 10 μM 4,4′-diisothiocyanatostilbene-2,2′-disulphonic acid (DIDS). Both amiloride and DIDS were able to inhibit the recovery of [Cl]i after an initial exposure to Na+- or Cl-free solution. The amiloride derivatives, benzamil (2 μM) and N-isobutyl-N-methylamiloride (MIBA), (10 μM) also lowered [Cl]i by similar amounts as 10 μM amiloride. Varying external K+ concentration ([K+]o) also affected [Cl]i. Increasing [K+]o increased [Cl]i, but decreasing [K+]o did not decrease [Cl]i. Instead, [Cl]i was also increased when [K+]o was lowered below the control value. Bumetanide (0.1 mM) lowered [Cl]i by only a small amount, while ouabain (1 mM) had no significant effect on [Cl]i. These data are consistent with current models of electrolyte transport in salivary ducts which include Cl channels, Na+ channels, and Na+/H+ exchangers in the apical membrane. The effects of low [K+]o can be interpreted in terms of a K+-dependent exit mechanism for Cl.

Key words

Salivary gland Striated ducts Intralobular ducts Intracellular Cl Fluorescence MQAE DIDS Amiloride 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • K. R. Lau
    • 1
  • R. L. Evans
    • 2
  • R. M. Case
    • 1
  1. 1.Department of Physiological SciencesUniversity of ManchesterManchesterUK
  2. 2.Clinical Investigation and Patient Care Branch, National Institute of Dental ResearchNational Institutes of HealthBethesdaUSA

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