Abstract
Tissue contents of intermediates of fatty acid metabolism were determined in isolated volume-overloaded rat hearts, 3 months after creation of an aorto-caval fistula. In the absence of any modification of blood carnitine, tissue levels of total carnitine were reduced by 33% in overloaded hearts compared to normal hearts. Total tissue CoA was unchanged. Fifteen minutes of whole-heart ischemia (i.e. a 50% reduction in coronary flow) did not increase levels of long-chain acyl esters of CoA and carnitine of the overloaded myocardium, in the presence of glucose as the only exogenous substrate. This was associated with lower than normal levels of long-chain acyl carnitine under normoxic conditions. The addition of exogenous palmitate (1.5 mM) resulted in an ischemia-induced accumulation of long-chain acyl-CoA and acyl carnitine in the overloaded heart although to a smaller extent than in the normal heart under similar perfusion conditions.
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References
Abu-Erreish GM, Neely JR, Whitmer JT, Whitman V, Sanadi DR (1977) Fatty acid oxidation by isolated perfused working hearts of aged rats. Am J Physiol 232:E258-E262
Bohmer T, Mølstad P (1980) Carnitine transport across the plasma membrane. In: Frenkel RA, McGarry JD (eds) Carnitine biosynthesis, metabolism and functions, Academic Press, New York, pp 73–89
Bremer J (1977) Carnitine and its role in fatty acid metabolism. Trends Biochem Sci 2:207–209
Cox RA, Hoppel CL (1974) Carnitine and trimethylaminobutyrate synthesis in rat tissues. Biochem J 142:699–701
Fawaz EN, Fawaz G, Von Dahl K (1962) Enzymatic estimation of phosphocreatine. Proc Soc Exptl Biol Med 109:38–43
Feuvray D (1981) Structural, functional, and metabolic correlates in ischemic hearts: effects of substrates. Am J Physiol 240:H391-H398
Feuvray D, Idell-Wenger JA, Neely JR (1979) Effects of ischemia on rat myocardial function and metabolism in diabetes. Circ Res 44:322–329
Feuvray D, Plouet J (1981) Relationship between structure and fatty acid metabolism in mitochondria isolated from ischemic rat hearts. Circ Res 48:740–747
Fossel ET, Ingawal JS (1982) Measurement of dynamic aspects of energy metabolism in the heart using 31P nuclear magnetic resonance spectroscopy. In: Cohen JS (ed) Noninvasive probes of tissue metabolism, Wiley, New York, pp 173–184
Furchgott RF, de Gubareff T (1956) The determination of inorganic phosphate and creatine phosphate in tissue extracts. J Biol Chem 233:337–342
Garland PB, Shepherd D, Yates DW (1965) Steady state concentrations of coenzyme A, acetyl coenzyme A and long chain fatty acyl coenzyme A in rat liver mitochondria oxidizing palmitate. Biochem J 97:587–594
Hatt PY, Rakusan K, Gastineau P, Laplace M, Cluzeaud F (1980) Aorto-caval fistula in the rat. An experimental model of heart volume overloading. Basic Res Cardiol 75:105–108
Mc Donough KH, Costello ME, Neely JR (1979) Control of triglyceride lipase activity in cardiac muscle. Fed Proc 38:894
Mc Garry JD, Foster DW (1976) An improved and simplified radioisotope assay for the determination of free and esterified carnitine. J Lipid Res 17:277–281
Moravec J (1980) Possible relationship between tissue levels of long-chain acyl CoA and the ability of the overloaded myocardium to reoxidize an excess of reduced pyridine nucleotide. FEBS Lett 113:134–138
Moravec J, Moravec M, Hatt PY (1981) Rate of pyridine nucleotide oxidation and cytochrome oxidase interaction with intracellular oxygen in hearts from rats with compensated volume overload. Pflügers Arch 392:106–114
Neely JR, Feuvray D (1981) Metabolic products and myocardial ischemia. Am J Pathol 102:282–291
Neely JR, Liebermeister H, Battersby EJ, Morgan HE (1967) Effect of pressure development on oxygen consumption by isolated rat heart. Am J Physiol 212:804–814
Neely JR, Rovetto MJ, Whitmer JT, Morgan HE (1973) Effects of ischemia on ventricular function and metabolism in the isolated working rat heart. Am J Physiol 225:651–658
Neely JR, Liedtke AJ, Whitmer JT, Rovetto MJ (1975) Relationship between coronary flow and adenosine triphosphate production from glycolysis and oxidative metabolism. In: Roy PE, Harris P (eds) Recent advances in studies on cardiac structure and metabolism, vol 8, University Park Press, pp 301–311
Opie LH (1968) Metabolism of the heart in health and disease. Am Heart J 76:685–698
Oram JF, Bennetch SL, Neely JR (1973) Regulation of fatty acid utilization in isolated perfused rat hearts. J Biol Chem 248:5299–5309
Revis NW, Cameron AJV (1979) Metabolism of lipids in experimental hypertrophic hearts of rabbits. Metabolism 28:601–613
Shug AL, Shrago E, Bittar M, Folts JD, Kokes JR (1975) Acyl-CoA inhibition of adenine nucleotide translocation in ischemic myocardium. Am J Physiol 228:689–692
Tanphaichitr V, Broquist HP (1974) Site of carnitine biosynthesis in the rat. J Nutr 104:1669–1673
Vary TC, Neely JR (1982) Characterization of carnitine transport in isolated perfused adult rat hearts. Am J Physiol 242:H585-H592
Whitmer JT, Idell-Wenger JA, Rovetto MJ, Neely JR (1978) Control of fatty acid metabolism in ischemic and hypoxic hearts. J Biol Chem 253:4305–4309
Wittels B, Spann JF (1968) Defective lipid metabolism in the failing heart. J Clin Invest 47:1787–1794
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Bowe, C., Nzonzi, J., Corsin, A. et al. Lipid intermediates in chronically volume-overloaded rat hearts. Pflugers Arch. 402, 317–320 (1984). https://doi.org/10.1007/BF00585516
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DOI: https://doi.org/10.1007/BF00585516