Virchows Archiv A

, Volume 400, Issue 2, pp 173–186 | Cite as

A morphometric analysis of the hypertrophy of experimental liver cirrhosis

  • Jei W. Ryoo
  • Robert J. Buschmann
Article

Summary

The objective of this study was to better elucidate the composition of the hypertrophic cirrhotic liver. We induced cirrhosis with hypertrophy in rats by simultaneous treatment with CCl4 and phenobarbital (PB) and devised a hierarchy of structure applicable to the sampling and morphometric analysis of untreated and PB-treated control livers and of cirrhotic livers. Our analysis demonstrated that the hepatomegaly attributable to cirrhosis is virtually the total result of an increase in the specific volume (ml/100 g body weight) of the non-parenchyma, most of which is connective tissue and vascular lumen volume. Inconsequential to the hepatomegaly but statistically significant were the volumetric increases found in the following parenchymal compartments: hepatocyte nucleus, Kupffer/endothelial cell nucleus, Ito cell nucleus and cytoplasm, and bile canaliculus. The change in the hepatocyte nucleus is the result of an increase in size rather than in number. Sinusoidal space is the only compartment that showed a significant decrease. This study shows the practicality and usefulness of applying morphometric methods to cirrhotic liver.

Key words

Liver cirrhosis experimental Carbon tetrachloride Hypertension portal Stereology Hepatomegaly 

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References

  1. Abercrombie M (1946) Estimation of nuclear population from microtome sections. Anat Rec 94:239–247Google Scholar
  2. Anthony PP, Ishak KG, Nayak NC, Poulsen HE, Scheuer PJ, Sobin LH (1978) The morphology of cirrhosis. Recommendations on definition, nomenclature, and classification by a working group sponsored by the World Health Organization. J Clin Pathol 31:395–414Google Scholar
  3. Bircher J, Blankart R, Halpern A, Häcki W, Laissue J, Preisig R (1973) Criteria for assessment of functional impairment in patients with cirrhosis of the liver. Eur J Clin Invest 3:72–85Google Scholar
  4. Blouin A, Bolender RP, Weibel ER (1977) Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study. J Cell Biol 72:441–455Google Scholar
  5. Burger PC, Herdson PB (1966) Phenobarbital-induced fine structural changes in rat liver. Am J Pathol 48:793–807Google Scholar
  6. Edmondson HA, Peters RL (1977) Liver. In: Anderson WAD, Kissane JM (eds) Pathology. 7th ed, CV Mosby, St. Louis, pp 1321–1438Google Scholar
  7. Galambos JT (1979) Cirrhosis. WB Saunders, Philadelphia, p 52Google Scholar
  8. Gundersen HJG (1977) Notes on the estimation of the numerical density of arbitrary profiles: the edge effect. J Microsc 111:219–223Google Scholar
  9. Jacques EA, Buschmann RJ, Layden TJ (1979) The histopathologic progression of vitamin A-induced hepatic injury. Gastroenterology 76:599–602Google Scholar
  10. Kent G, Gay S, Inouye T, Bahu R, Minick OT, Popper H (1976) Vitamin A-containing lipocytes and formation of type III collagen in liver injury. Proc Natl Acad Sci USA 73:3719–3722Google Scholar
  11. Koo A, Liang IYS, Cheng KK (1976) Effect of the ligation of hepatic artery on the microcirculation in the cirrhotic liver in the rat. Aust J Exp Biol Med Sci 54:287–295Google Scholar
  12. Leevy CM, Popper H, Sherlock S (Criteria committee) (1976) Diseases of the liver and biliary tract: standardization of nomenclature, diagnostic criteria and diagnostic methodology. Fogarty International Center Proceedings, No 22 DHEW Publication No (NIH) 76-725. Government Printing Office, Washington, DC, pp 17–24Google Scholar
  13. Ludwig J, Elveback LR (1972) Parenchyma weight changes in hepatic cirrhosis: a morphometric study and discussion of the method. Lab Invest 26:338–343Google Scholar
  14. Massey ED, Butler WH (1979) Zonal changes in the rat liver after chronic administration of phenobarbitone: an ultrastructural, morphometric and biochemical correlation. Chem Biol Interact 24:329–344Google Scholar
  15. McGee JO, Patrick RS (1972) The role of perisinusoidal cells in hepatic fibrogenesis: an electron microscopic study of acute carbon tetrachloride liver injury. Lab Invest 26:429–440Google Scholar
  16. McLean EK, McLean AEM, Sutton PM (1969) Instant cirrhosis: an improved method for producing cirrhosis of the liver in rats by simultaneous administration of carbon tetrachloride and phenobarbitone. Br J Exp Path 50:502–506Google Scholar
  17. McSween RNM (1976) Liver, biliary tract and exocrine pancreas. In: Anderson JR (ed) Muir's Textbook of Pathology. 10th ed Year Book, Chicago, pp 601–659Google Scholar
  18. Morselt AFW, Freni SC, Ortlep FM (1976) Quantitative histological aspects of liver cirrhosis. Beitr Pathol 159:195–206Google Scholar
  19. Nakamura T, Nakamura S, Aikawa T, Tazawa T, Suzuki O, Suzuki T (1965) Morphological classification of liver cirrhosis based upon measurement of per cent of interstitial tissue in liver biopsy specimens. Tohoku J Exp Med 87:110–122Google Scholar
  20. Nuber R, Teutsch HF, Sasse D (1980) Metabolic zonation in thioacetamide-induced liver cirrhosis. Histochemistry 69:277–288Google Scholar
  21. Pirttiaho HI, Sotaniemi EA, Ahlqvist J, Pitkänen U, Pelkonen RO (1978) Liver size and indices of drug metabolism in alcoholics. Eur J Clin Pharmacol 13:61–67Google Scholar
  22. Popper H (1977) Pathologic aspects of cirrhosis: a review. Am J Pathol 87:228–264Google Scholar
  23. Rappaport AM (1976) The microcirculatory acinar concept of normal and pathological hepatic structure. Beitr Pathol 157:215–243Google Scholar
  24. Romagna F, Zbinden G (1981) Distribution of nuclear size and DNA content in serial liver biopsies of rats treated with N-nitrosomorpholine, phenobarbital and butylated hydroxytoluene. Exp Cell Biol 49:294–305Google Scholar
  25. Scherle W (1970) A simple method for volumetry of organs in quantitative stereology. Microskopie 26:57–60Google Scholar
  26. Sherlock S (1981) Diseases of the liver and biliary system. 6th ed, Blackwell, Oxford, p 331Google Scholar
  27. Sokal RR, Rohlf FJ (1981) Biometry: The Principles and Practice of Statistics in Biological Research. 2nd ed. WH Freeman, San Francisco, pp 232–242Google Scholar
  28. Weibel ER (1979) Stereological Methods, vol 1, Practical Methods for Biological Morphometry. Academic Press, London New York Toronto Sydney San FranciscoGoogle Scholar
  29. Wood AJJ, Villeneuve JP, Branch RA, Rogers LW, Shand DG (1979) Intact hepatocyte theory of impaired drug metabolism in experimental cirrhosis in the rat. Gastroenterology 76:1358–1362Google Scholar
  30. Zimmon DS (1977) The hepatic vasculature and its response to hepatic injury: a working hypothesis. Yale J Biol Med 50:497–506Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Jei W. Ryoo
    • 1
  • Robert J. Buschmann
    • 1
  1. 1.Departments of PathologyUniversity of Illinois at the Medical Center and Veterans Administration West Side Medical CenterChicagoUSA

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