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Über den Mechanismus der Potenzierung der Katecholaminwirkung nach chronisch postganglionärer sympathischer Denervierung

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Summary

The influence of chronic postganglionic sympathetic denervation upon uptake and effects of administereddl-[H]3-Noradrenaline ([H]3-NA) has been investigated using the isolated perfused spleen preparation. The total radioactivity and [H]3-NA was determined in the tissues and in the perfusate. The maximal splenic contraction was measured as change in volume. 108, 216, and 432 nanogramsdl-7-[H]3-NA were infused into the innervated tissues, 54, 108, and 216 nanograms into the denervated. The duration of the infusions in each case was 2 min, and the perfusate was collected for 29 min during and after each infusion.

In the innervated preparations, 53.5% of the radioactivity was found in the tissues, 41% in the perfusate and 5.4% in the bath fluid. In denervated preparations, only 5.6% of the infused radioactivity was found in the tissues, whilst 85% was recovered in the perfusate and 8% in the bath fluid. In the innervated tissues 92% of the radioactivity was recovered in [H]3-NA, whereas in the denervated tissues only 50% was present in [H]3-NA.

In the perfusate of innervated tissues 74% of the total radioactivity was present in [H]3-NA, whilst in the perfusate of the denervated tissues 84% of the total radioactivity was recovered in [H]3-NA.

The sensitivity of the denervated spleens was 2,78 times higher towardsdl-[H]3-NA as compared with the innervated organs. In order to reach similar concentrations of [H]3-NA in the perfusates of the first sampling period, in which where the spleen contractions occur, 2,13 times moredl-[H]3-NA had to be infused into the innervated spleens than in the case of the denervated organs. The cause of the difference between innervated and denervated tissues in respect to sensitivity on the one hand, and rise in NA-concentration in the perfusate on the other hand is discussed.

It is concluded that the supersensitivity following chronic postganglionic sympathetic denervation is produced by the decreased ability of the tissues to take up Noradrenaline into the sympathetic nerve terminale and thereby inactivate it.

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität Wien, Austria

    G. Hertting, J. Suko, S. Widhalm & I. Harbich

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  1. G. Hertting
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  2. J. Suko
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  3. S. Widhalm
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  4. I. Harbich
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Additional information

Über einen Teil der Ergebnisse wurde auf der sechsten Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft in Mainz 1965 berichtet (Naunyn-Schmiedebergs Arch. exp. Path. Pharmak.251, 164 (1965).

Diese Untersuchungen wurden mit der Unterstützung des Public Health Service Research Grant No. H-7025 des National Heart Institute, Bethesda, USA, durchgeführt.

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Hertting, G., Suko, J., Widhalm, S. et al. Über den Mechanismus der Potenzierung der Katecholaminwirkung nach chronisch postganglionärer sympathischer Denervierung. Naunyn-Schmiedebergs Arch. Pharmak. u. Exp. Path. 256, 40–54 (1967). https://doi.org/10.1007/BF00585357

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