Pflügers Archiv

, Volume 368, Issue 3, pp 245–252 | Cite as

Coupling between proximal tubular transport processes

Studies with ouabain, SITS and HCO3-free solutions
  • K. J. Ullrich
  • G. Capasso
  • G. Rumrich
  • F. Papavassiliou
  • S. Klöss


The rate of active transport by the proximal renal tubule of amino acid (l-histidine), sugar (α-methyl-d-glycoside), H+ ions (glycodiazine), phosphate and para-aminohippurate was evaluated by measuring the zero net flux concentration difference (Δc) of these substances. In the case of calcium the electrochemical potential differenceΔc +zFciΔϕ/RT) was the criterion employed. The rate of isotonic Na+-absorption (JNa) was measured with the shrinking droplet method. The effect of ouabain on the transport of these substances was tested in the golden hamster and the effect of SITS (4-acetamido-4′isothiocyanatostilbene 2,2′-disulfonic acid) was observed in rats.

Ouabain (1 mM) applied peritubularly incompletely inhibited JNa (80%), but in combination with acetazolamide (0.2 mM) the inhibition was almost complete (93%). In addition, ouabain inhibited the sodium coupled (secondary active) transport processes ofl-histidine, α-methyl-d-glycoside, calcium and phosphate by more than 75%. It did not affect H+ (glycodiazine) transport and PAH transport was only slightly affected.

When SITS (1 mM) was applied from both sides of the cell it inhibited H+ (glycodiazine) transport by 72% and reduced JNa by 38% when given from only the peritubular cell side. SITS (1 mM), however, had no significant affect on H+ secretion and sodium reabsorption if it was applied from only the luminal side. Furthermore it had no affect on the other transport processes tested, regardless of the cell side to which it was applied.

When the HCO 3 buffer or physically related buffers were omitted from the perfusate the absorption of Na+ was reduced by 66%, phosphate by 44%, andl-histidine by 15%. All the other transport processes tested were not significantly affected.

The data are consistent with the hypothesis that the active transport processes of histidine, α-methyl-d-glycoside and phosphate, which are located in the brush border, are driven by a sodium gradient which is abolished by ouabain. This may also apply to the Na+-Ca2+ countertransport located at the contraluminal cell side. The residual Na+ transport remaining in the presence of ouabain is likely to be passively driven by the continuing H+ transport which probably is driven directly by ATP. SITS seems to inhibit the exit step of HCO 3 from the cell and secondary to that, the luminal H+-Na+ exchange and consequently the Na+ reabsorption. In the absence of HCO 3 buffer in the perfusates the luminal H+-Na+ exchange seems to be affected and the pattern of inhibition of the other transport processes is almost the same as with SITS. The different effects onPi reabsorption observed under these conditions might be explained by possible variations in intracellular pH.

Key words

Renal tubule H+ ion secretion Na+ coupled transport Ouabain SITS 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Aubert, L., Motais, R.: Molecular features of organic anion permeability in ox red blood cell. J. Physiol. (Lond.)246, 159–179 (1975)Google Scholar
  2. 2.
    Baumann, K., Rumrich, G., Papavassiliou, F., Klöss, S.: pH dependence of phosphate reabsorption in the proximal tubule of rat kidney. Pflügers Arch.360, 183–187 (1975)Google Scholar
  3. 3.
    Baumann, K., de Rouffignac, C., Roinel, N., Rumrich, G., Ullrich, K. J.: Renal phosphate transport: Inhomogeneity of local proximal transport rates and sodium dependence. Pflügers Arch.356, 287–297 (1975)Google Scholar
  4. 4.
    Berner, W., Kinne, R.: Transport of p-aminohippuric acid by plasma membrane vesicles isolated from rat kidney cortex. Pflügers Arch.361, 269–277 (1976)Google Scholar
  5. 5.
    Burg, M. B., Green, N.: Role of monovalent ions in the reabsorption of fluid by isolated perfused proximal renal tubules of the rabbit. Kidney Int.10, 221–228 (1976)Google Scholar
  6. 6.
    Burg, M. B., Orloff, J.: Effect of strophanthidin on electrolyte content and PAH accumulation of rabbit kidney slices. Amer. J. Physiol.202, 565–571 (1962)Google Scholar
  7. 7.
    Chung, S. T., Park, Y. S., Hong, S. K.: Effect of cations on transport of weak organic acids in rabbit kidney slices. Amer. J. Physiol.219, 30–33 (1970)Google Scholar
  8. 8.
    Dantzler, W. H.: Effects of K, Na, and ouabain on urate and PAH uptake by snake and chicken kidney slices. Amer. J. Physil.217, 1510–1519 (1969)Google Scholar
  9. 9.
    Dantzler, W. H., Bentley, S. K.: Low Na+ effects on PAH transport and permeabilities in isolated snake renal tubules. Amer. J. Physiol230, 256–262 (1976)Google Scholar
  10. 10.
    Ehrenspeck, G., Brodsky, W. A.: Effect of 4-acetamido-4′-isothiocyano-2,2-disulfonic stilbene on ion transport in turtle bladders. Biochim. biophys. Acta (Amst.)419, 555–558Google Scholar
  11. 11.
    Evers, J., Murer, H., Kinne, R.: Phenylalanine uptake in isolated renal brush border vesicles. Biochim. biophys. Acta (Amst.)426, 598–615 (1976)Google Scholar
  12. 12.
    Frömter, E., Rumrich, G., Ullrich, K. J.: Phenomenological description of Na+Cl and HCO3-absorption from proximal tubules of the rat kidney. Pflügers Arch.343, 189–220 (1973)Google Scholar
  13. 13.
    Frömter, E., Geßner, K.: Active transport potentials, membrane diffusion potentials and streaming potentials across rat kidney proximal tubule. Pflügers Arch.351, 85–98 (1974)Google Scholar
  14. 14.
    Frömter, E., Geßner, K.: Effect of inhibitors and diuretics on electrical potential difference in rat kidney proximal tubule. Pflügers Arch.357, 209–224 (1975)Google Scholar
  15. 15.
    Frömter, E., Sato, K., Geßner, K.: Electrical studies on the mechanism of H+/HCO3 transport across rat kidney proximal tubule. In: Proc. VI. Internat. Congress of Nephrology (S. Giovannetti, V. Bonomini, and G. D'Amico, eds.), pp. 108–112. Basel: S. Karger 1976Google Scholar
  16. 16.
    Gerencser, G. A., Park, Y. S., Hong, S. K.: Sodium influence upon the transport kinetics of p-aminohippurate in rabbit kidney slices. Proc. Soc. exp. Biol. (N.Y.)144, 440–444 (1973)Google Scholar
  17. 17.
    Grantham, J. J., Qualizza, P. B., Irwin, R. L.: Net fluid secretion in proximal straight renal tubules in vitro. Role of PAH. Amer. J. Physiol.226, 191–197 (1974)Google Scholar
  18. 18.
    Green, R., Giebisch, G.: Ionic requirements of proximal tubular Na+ transport. I. Bicarbonate and chloride. Amer. J. Physiol.229, 1205–1215 (1975)Google Scholar
  19. 19.
    Green, R., Giebisch, G.: Ionic requirements of proximal tubular Na+ transport. II. Hydrogen ion. Amer. J. Physiol229, 1216–1226 (1975)Google Scholar
  20. 20.
    Györy, A. Z.: Reexamination of the split oil droplet method as applied to kidney tubules. Pflügers Arch.324, 328–343 (1971)Google Scholar
  21. 21.
    Györy, A. Z., Brendel, U., Kinne, R.: Effect of cardiac glycosides and sodium ethacrynate on transepithelial sodium transport in in-vivo micropuncture experiments and on isolated plasma membrane Na+K+-ATPase in vitro of the rat. Pflügers Arch.335, 287–296 (1972)Google Scholar
  22. 22.
    Hoffmann, N., Thees, M., Kinne, R.: Phosphate transport by isolated renal brush border vesicles. Pflügers Arch.362, 147–156 (1976)Google Scholar
  23. 23.
    Hoshi, T., Hayashi, M.: Role of sodium ions in phenol red transport by renal tubules of the goldfish. Jap. J. Physiol.20, 683–696 (1970)Google Scholar
  24. 24.
    Kinne, R., Schmitz, J. E., Kinne-Saffran, E.: The localization of the Na+-K+-ATPase in the cells of rat kidney cortex. A study on isolated plasma membranes. Pflügers Arch.329, 191–206 (1971)Google Scholar
  25. 25.
    Kinne-Saffran, E., Kinne, R.: Presence of bicarbonate stimulated ATPase in the brush border microvillus membranes of the proximal tubule. Proc. Soc. exp. Biol. (N.Y.)146, 751–753 (1974)Google Scholar
  26. 26.
    Kinne, R., Murer, H., Kinne-Saffran, E., Thees, M., Sachs, G.: Sugar transport by renal plasma membrane vesicles. Characterization of the system in the brush-border microvilli and basallateral plasma membranes. J. Membr. Biol.21, 375–395 (1975)Google Scholar
  27. 27.
    Lingard, J., Rumrich, G., Young, J. A.: Reabsorption ofl-glutamine andl-histidine from various regions of the rat proximal convolution studied by stationary microperfusion: Evidence that the proximal convolution is not homogenous. Pflügers Arch.342, 1–12 (1972)Google Scholar
  28. 28.
    Maude, D. L.: The role of bicarbonate in proximal tubular sodium chloride transport. Kidney Int.5, 253–260 (1974)Google Scholar
  29. 29.
    McDonough, A., Hong, S. K.: Na+-K+-ATPase and PAH transport in the renal cortex. Fed. Proc.35, 848 (1976)Google Scholar
  30. 30.
    Murer, H., Hopfer, U., Kinne, R.: Sodium/proton antiport in brush-border-membrane vesicles isolated from rat small intestine and rat kidney. Biochem. J.154, 597–604 (1976)Google Scholar
  31. 31.
    Samarzija, I., Frömter, E.: Electrical studies on amino acid transport across bruch-border membrane of rat proximal tubule in vivo. Pflügers Arch.359, R 119 (1975)Google Scholar
  32. 32.
    Ullrich, K. J., Frömter, E., Baumann, K.: Micropuncture and microanalysis in kidney physiology. In: Laboratory techniques in membrane biophysics (H. Passow and R. Stämpfli, eds.), pp. 106–129. Berlin-Heidelberg-New York: Springer 1969Google Scholar
  33. 33.
    Ullrich, K. J., Radtke, H. W., Rumrich, G.: The role of bicarbonate and other buffers on isotonic fluid absorption in the proximal convolution of the rat kidney. Pflügers Arch.330, 149–161 (1971)Google Scholar
  34. 34.
    Ullrich, K. J., Sato, K., Rumrich, G.: Coupling of transport processes across the brush border of proximal renal tubule. In: Transport mechanism in epithelia (H. H. Ussing and N. A. Thorn, eds.), pp. 560–571, Copenhagen: Munksgaard; New York: Academic Press 1973Google Scholar
  35. 35.
    Ullrich, K. J.: Permeability characteristics of the mammalian nephron. Handbook of Physiol., Section 8, Renal Physiology (J. Orloff and R. W. Berliner, eds.), pp. 377–398. Washington D. C.: Amer. Physiol. Soc. 1973Google Scholar
  36. 36.
    Ullrich, K. J.: Recent progress in renal physiology. Transport of hexoses, amino acid, phosphate and para-aminohippurate in the proximal tubule. Soc. Ital. Fisiol., pp. 1–26, Atti del Congressor Riva del Garda 1974Google Scholar
  37. 37.
    Ullrich, K. J., Rumrich, G., Klöss, S.: Specificity and sodium dependence of the active sugar transport in the proximal convolution of the rat kidney. Pflügers Arch.351, 35–48 (1974a)Google Scholar
  38. 38.
    Ullrich, K. J., Rumrich, G., Klöss, S.: Sodium dependence of the amino acid transport in the proximal convolution of the rat kidney. Pflügers Arch.351, 49–60 (1974b)Google Scholar
  39. 39.
    Ullrich, K. J., Rumrich, G., Baumann, K.: Renal proximal tubular buffer-(glycodiazine) transport: inhomogeneity of local transport rate, dependence on sodium, effect of inhibitors and chronic adaptation. Pflügers Arch.357, 149–163 (1975)Google Scholar
  40. 40.
    Ullrich, K. J., Rumrich, G., Klöss, S.: Active Ca2+ reabsorption in the proximal tubule of the rat kidney. Dependence on Na+ and buffer transport. Pflügers Arch.364, 223–228 (1976)Google Scholar
  41. 41.
    Vogel, G., Lauterbach, F., Kröger, W.: Die Bedeutung des Natriums für die renalen Transporte von Glucose und Para-Aminohippursäure. Pflügers Arch. ges. Physiol.283, 151–159 (1965)Google Scholar
  42. 42.
    Vogel, G., Kröger, W.: Die Bedeutung des Transportes, der Konzentration und der Darbietungsrichtung von Na+ für den tubulären Glucose- und PAH-Transport. Pflügers Arch. ges. Physiol.288, 342–358 (1966)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • K. J. Ullrich
    • 1
  • G. Capasso
    • 1
  • G. Rumrich
    • 1
  • F. Papavassiliou
    • 1
  • S. Klöss
    • 1
  1. 1.Max-Planck-Institut für BiophysikFrankfurt/MainFederal Republic of Germany

Personalised recommendations