Summary
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1.
Extracellular potassium, bicarbonate, ouabain, dipyridamole and the Na+ distribution between red cells and plasma influence Li+ transport across the human red cell membrane. The significance of these parameters for theintraindividual variability of the steady-state ratio of external to internal Li+ was studied in vitro.
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2.
Elevation of external K+ in the physiological concentration rangeincreases the steady-state distribution ratio Li /+ e /Li /+ i indirectly by increasing the ratio Na /+ e /Na /+ i through activation of the Na+−K+ pump, and directly by inhibiting ouabain-sensitive Li+ uptake.
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3.
A rise in bicarbonate concentrationdecreases the Li+ ratio directly by accelerating Li+ uptake through a leak, and indirectly by increasing the Na+ leak, thus reducing the Na+ ratio.
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4.
Dipyridamole blocks both bicarbonate effects.
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5.
Ouabain decreases the Na+ ratio and inhibits Li+ uptake by the Na+−K+ pump, thereby exerting two opposite effects on the Li+ distribution ratio.
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6.
The results confirm the previous observation that the steady-state Li+ distribution depends strongly on the Na+ distribution ratio, i.e., the driving force for Na+-dependent Li+ uphill countertransport. It is concluded that the Na+ distribution between red cells and plasma and the concentrations of K+ and bicarbonate in plasma need to be considered as factors influencing the in vivo Li+ distribution. However, the considerableinterindividual differences of Li+ distribution cannot be ascribed to variations in these parameters.
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Duhm, J., Becker, B.F. Studies on the lithium transport across the red cell membrane. Pflugers Arch. 368, 203–208 (1977). https://doi.org/10.1007/BF00585197
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DOI: https://doi.org/10.1007/BF00585197