Summary
The kinetics of vascular smooth muscle activity was studied by means of afterloaded isotonic contractions of the tetanized rat portal vein at varied pH (8.0–5.9), pCa (3.4–2.1), and during noradrenaline incubation (0.4 μg/ml). Under control conditions (pH 7.3, pCa 2.6) the following parameters of the force velocity relation were calculated:a of Hill's equation (relating to the isometric peak tension)=0.36;b (relating to the actual muscle length)=0.19 ML/s;V M (relating to the actual muscle length)=0.56 ML/s. Within the range of pCa between 2.0 and 3.2 the amount of force generation (=ΔP) depended on the extracellular calcium level whereas the extrapolated velocity of shortening of the unloaded preparation (=V M) did not. Also pH changes between 8.0 and 6.8 as well as noradrenaline incubation at a pH of 5.9 affectedΔP quite considerably, butV M only scarcely. At a pH of 6.3, however,V M was distinctly diminished, and a reduced calcium sensitivity of the ATPase was inferred from the shift of ED50 of extracellular calcium from 0.66 mM Ca at a pH of 7.3 to 1.56 mM Ca at a pH of 6.3 (P<0.0005). It is concluded from these results that the experimental conditions—pCa between 2.0 and 3.2, pH between 8.0 and 6.8, and noradrenaline added at a pH of 5.9—obviously change the intracellular calcium concentration which influences the number of activated interaction sites rather than the velocity of crossbridge movement.
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References
Baguet, F., The catch-state in glycerol extracted fibres from a lamellibranch smooth muscle (ABRM). Pflügers Arch.340, 19–34 (1973)
Bárány, M.: ATPase activity of myosin correlated with speed of muscle shortening. J. gen. Physiol.50, 197–216 (1967)
Betz, E., Enzenroß, H. G., Vlahov, V.: Interaction of H+ and Ca2+ in the regulation of local pial vascular resistance. Pflügers Arch.343, 79–88 (1973)
Bilek, I., Laven, R., Peiper, U., Regnat, K.: The effect of verapamil on the response to noradrenaline or to potassium-depolarization in isolated vascular strips. Microvasc. Res.7, 181–189 (1974)
Bohr, D. F., Uchida, E.: Activation of vascular smooth muscle, pp. 133–145. Chicago-London: The University of Chicago Press 1969
van Breemen, C., Farinas, B. R., Gerba, P., McNaughton, E. D.: Excitation-contration coupling in rabbit aorta studied by the lanthanum method for measuring cellular calcium influx. Circulat. Res.30, 44–54 (1972)
Brutsaert, D. L., Claes, V. A., Goethals, M. A.: Effect of calcium on force-velocity-length relations of heart muscle of the cat. Circulat. Res.32, 385–392 (1973)
Close, R., Hoh, J. F. Y.: Influence of temperature on isometric contractions of rat skeletal muscles. Nature (Lond.)217, 1179–1180 (1968)
Cohen, S., Green, F.: Force-velocity characteristics of esophageal muscle: effect of acetylcholine and norepinephrine. Amer. J. Physiol.226, 1250–1256 (1974)
Devine, C. E., Somlyo, A. V., Somlyo, A. P.: Sarcoplasmatic reticulum and excitation-contraction coupling in mammalian smooth muscles. J. Cell Biol52, 690–718 (1972)
Edman, K. A. P., Mattiazzi, A., Nilsson, E.: The influence of temperature on the force-velocity relationship in rabbit papillary muscle. Acta physiol. scand.90, 750–756 (1974)
Ferrari, M.: Interactions between Ca and some myolytic agents on depolarized vascular smooth muscle. J. Pharm. Pharmacol.22, 71–72 (1970)
Godfraind, T., Kaba, A.: Blockade or reversal of the contraction induced by calcium and adrenaline in depolarized arterial smooth muscle. Brit. J. Pharmacol.36, 549–560 (1969)
Grün, G., Fleckenstein, A.: Die elektromechanische Entkoppelung der glatten Gefäßmuskulatur als Grundprinzip der Coronardilatation durch 4-(2′-Nitrophenyl)-2,6-dimethyl-1,4-dihydropyridin-3,5-dicarbonsäure-dimethylester (BAY a 1040, Nifedipine). Arzneimittel-Forsch. (Drug Res.)22, 334–344 (1972)
Gün, G., Weder, U., Fleckenstein, A.: The mutual antagonism between H+ and Ca2+ in the control of vascular tone and autoregulation. Pflügers Arch.335, R 10 (1972)
Hellstrand, P., Johansson, B.: The force-velocity relation in venous smooth muscle. Acta physiol. scand.91, 45 A (1974)
Hellstrand, P., Johansson, B., Ringberg, A.: Influence of extracellular calcium on isometric force and velocity of shortening in depolarized venous smooth muscle. Acta physiol. scand.84, 528–537 (1972)
Herlihy, J. T., Murphy, R. A.: Force-velocity and series elastic characteristics of smooth muscle from the hog carotid artery. Circulat. Res.34, 461–466 (1974)
Hill, A. V.: The influence of temperature on the tension developed in an isometric twitch. Proc. roy. Soc. B138, 349–354 (1951)
Hinke, J. A. M., Wilson, M. L., Burnham, S. C.: Calcium and the contractility of arterial smooth muscle. Amer. J. Physiol.206, 211–215 (1964)
Hudgins, P. M., Weiss, G. B.: Differential effects of calcium removal upon vascular smooth muscle contraction induced by norepinephrine, histamine, and potassium. J. Pharmacol. exp. Ther.159, 91–97 (1968)
Johansson, B.: Active state in the smooth muscle of the rat portal vein in relation to electrical activity and isometric force. Circulat. Res.32, 246–258 (1973)
Jhamandas, K. H., Nash, C. W.: Effects of inorganic anions on the contractility of vascular smooth muscle. Canad. J. Pharmacol.45, 675–682 (1967)
Julian, F. J.: The effect of calcium on the force velocity relation of briefly glycerinated frog muscle fibers. J. Physiol. (Lond.)218, 117–145 (1971)
Katz, A. M.: Contractile proteins of the heart. Physiol. Rev.50, 63–158 (1970)
Katz, A. M., Goodhart, P. J., Goodhart, H. L.: Calcium and the cardiac contractile proteins. In: Calcium and the heart (P. Harris and L. H. Opie, eds.). London-New York: Academic Press 1971
Laven, R., Peiper, U.: Force-velocity relations in a vascular smooth muscle preparation. Pflügers Arch.347, R16 (1974)
Mrwa, U., Achtig, I., Rüegg, J. C.: pH-effect on the actomyosin system of vascular smooth muscle. Pflügers Arch.335, R58 (1972)
Murphy, R. A., Bohr, D. F., Newman, D. L.: Arterial actomyosin: Mg, Ca, and ATP ion dependencies for ATPase activity. Amer. J. Physiol.217, 666–673 (1969)
Peiper, U., Laven, R., Ehl, M.: Force velocity relationships in vascular smooth muscle: the influence of temperature. Pflügers Arch.356, 33–45 (1975)
Podolsky, R. J., Teichholz, L. E.: The relation between calcium and contraction kinetics in skinned muscle fibres. J. Physiol. (Lond.)211, 19–35 (1970)
Rüegg, J. C.: Smooth muscle tone. Physiol. Rev.51, 201–248 (1971)
Schädler, M.: Proportionale Aktivierung von ATPase-Aktivität und Kontraktionsspannung durch Calciumionen in isolierten contractilen Strukturen verschiedener Muskelarten. Pflügers Arch.296, 70–90 (1967)
Sonnenblick, E. H.: Force-velocity relations in mammalian heart muscle. Amer. J. Physiol.202, 931–939 (1962)
Thames, M. D., Teichholz, L. E., Podolsky, R. J.: Ionic strength and the contraction kinetics of skinned muscle fibres. J. gen. Physiol.63, 509–530 (1974)
Wei, E. P., Thames, M. D., Kontos, H. A., Patterson, J. L.: Inhibition of the vasodilator effect of hypercapnic acidosis by hypercalcaemia in dogs and rats. Circulat. Res.35, 890–895 (1974)
Wise, R. M., Rondinone, J. F., Briggs, F. N.: Effect of calcium on force-velocity characteristics of glycerinated skeletal muscle. Amer. J. Physiol.221, 973–979 (1971)
Yeatman, L. A., Parmley, W. W., Sonnenblick, E. H.: Effects of temperature on series elasticity and contractile element motion in heart muscle. Amer. J. Physiol.217, 1030–1034 (1969)
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A preliminary report of some of the results was given at the 44th Meeting of the Deutsche Physiologische Gesellschaft, Bochum (Pflügers Arch.355, R24, 1975)
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Peiper, U., Ehl, M., Johnson, U. et al. Force velocity relations in vascular smooth muscle: The influence of pH, pCa, and noradrenaline. Pflugers Arch. 364, 135–141 (1976). https://doi.org/10.1007/BF00585181
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DOI: https://doi.org/10.1007/BF00585181