Pflügers Archiv

, Volume 397, Issue 1, pp 54–56 | Cite as

Calmodulin-dependent Ca2+ transport in erythrocytes of spontaneously hypertensive rats

  • S. N. Orlov
  • N. I. Pokudin
  • Yu. V. Postnov
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology

Abstract

Ca2+ transport in inside-out vesicles of the erythrocyte membranes of spontaneously hypertensive rats (SHR) was studied. In the presence of calmodulin, the rate of45Ca uptake by the erythrocyte membranes of SHR was half that of erythrocyte membranes of the normotensive control group. It is suggested that the decrease of the rate of calmodulin-dependent calcium transport across the plasma membrane may be a cause for the increased intracellular calcium content found in some tissues of spontaneously hypertensive rats in previous studies.

Key words

Spontaneous hypertension Erythrocytes Calcium pump Calmodulin 

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References

  1. 1.
    Aoki K, Ikedo N, Yamashita K, Tasumi K, Sato I, Hotta K (1974) Cardiovascular contraction in spontaneously hypertensive rats: Ca2+ interaction of myofibrils and subcellular membrane of heart and arterial smooth muscle. Jpn Cir J 38:1115–1119Google Scholar
  2. 2.
    Devynck MA, Pernollet MG, Nunez AM, Meyer P (1981) Calcium binding alteration in plasma membrane from various tissues of spontaneously hypertensive rats. Clin Exp Hyper 3:797–808Google Scholar
  3. 3.
    Limas CJ, Cohn JN (1977) Defective calcium transport by cardiac sarcoplasmic reticulum in spontaneously hypertensive rats. Cir Res 40, Suppl 1:62–69Google Scholar
  4. 4.
    Losse H, Zidek W, Zumkley H, Wessels F, Vetter H (1981) Intracellular Na+ as a genetic marker of essential hypertension. Clin Exp Hyper 3:627–640Google Scholar
  5. 5.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RI (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275Google Scholar
  6. 6.
    Macintyre J, Breen JW (1978) Stimulation of calcium transport in inside-out vesicles of human erythrocyte membranes by a soluble cytoplasmic activator. Biochim Biophys Acta 510:373–377Google Scholar
  7. 7.
    Miledi R (1973) Transmitter release induced by injection of calcium in nerve terminals. Proc R Soc Lond Biol Sci 183:421–425Google Scholar
  8. 8.
    Nigli V, Adunyah ES, Penniston JT, Carafoli E (1981) Purified (Ca2+−M2+)-ATPase of the erythrocyte membrane. Reconstitution and effect of calmodulin and phospholipids. J Biol Chem 256:395–401Google Scholar
  9. 9.
    Orlov SN, Gulak PV, Karagodina ZV, Postnov YuV (1981) The properties of the erythrocyte membrane structure in spontaneous genetic hypertension. Cardiology (Moscow) 21, 7:108–112Google Scholar
  10. 10.
    Orlov SN, Postnov YuV (1980) Calcium accumulation and calcium binding by the cell membranes of cardiomyocytes and smooth muscle of aorta in spontaneously hypertensive rats. Clin Sci 59: 207s-209sGoogle Scholar
  11. 11.
    Postnov YuV, Orlov SN (1979) Features of intracellular calcium distribution in the adipose tissue of spontaneously hypertensive rats (SHR). Experientia 35:1480–1481Google Scholar
  12. 12.
    Postnov YuV, Orlov SN (1980) Evidence of altered calcium accumulation and calcium binding by the membranes of adipocytes in spontaneously hypertensive rats. Pflügers Arch 385:85–89Google Scholar
  13. 13.
    Postnov YuV, Orlov SN, Pokudin NI (1979) Decrease of calcium binding by the red blood cell membrane in spontaneously hypertensive rats and in essential hypertension. Pflügers Arch 379: 191–195Google Scholar
  14. 14.
    Postnov YuV, Orlov SN, Pokudin NI (1980) Alteration of intracellular calcium distribution in the adipose tissue of human patients with essential hypertension. Pflügers Arch 388:89–91Google Scholar
  15. 15.
    Postnov YuV, Orlov SN, Shevchenko AS (1977) Calcium ion binding and Na−K-ATPase activity in erythrocytes of patients with essential hypertension. Bull Exp Biol Med (Moscow) 84:7:41–44Google Scholar
  16. 16.
    Rasmussen H, Goodman DB (1978) Relation between calcium and cyclic nucleotides in cell activation. Physiol Rev 57:421–510Google Scholar
  17. 17.
    Romero RP (1976) Role of membrane bound Ca in ghost permeability to Na and K. J Membr Biol 29:329–343Google Scholar
  18. 18.
    Schatzmann H (1975) Active calcium transport across the plasma membrane of erythrocytes, In: Carafoli E, Clementi F, Drabikowski W, Magretti A (eds) Calcium transport in contraction and secretion. Amsterdam Oxford, pp 45–49Google Scholar
  19. 19.
    Steck TL (1974) Preparation of impermeable inside-out and right-side-out vesicles from erythrocyte membrane. In: Korn E (ed) Methods in membrane biology. Plenum Press, New York London, pp 245–281Google Scholar
  20. 20.
    Wei Wu-J, Janis RA, Daniel EE (1976) Studies of subcellular fraction from mesenteric arteries of spontaneously hypertensive rats: alterations in both calcium uptake and enzyme activities. Blood Vessels 13:293–308Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • S. N. Orlov
    • 1
  • N. I. Pokudin
    • 1
  • Yu. V. Postnov
    • 1
  1. 1.Central Research Laboratory of the Ministry of Public Health of the USSRMoscowUSSR

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