Abstract
Ca2+ transport in inside-out vesicles of the erythrocyte membranes of spontaneously hypertensive rats (SHR) was studied. In the presence of calmodulin, the rate of45Ca uptake by the erythrocyte membranes of SHR was half that of erythrocyte membranes of the normotensive control group. It is suggested that the decrease of the rate of calmodulin-dependent calcium transport across the plasma membrane may be a cause for the increased intracellular calcium content found in some tissues of spontaneously hypertensive rats in previous studies.
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Aoki K, Ikedo N, Yamashita K, Tasumi K, Sato I, Hotta K (1974) Cardiovascular contraction in spontaneously hypertensive rats: Ca2+ interaction of myofibrils and subcellular membrane of heart and arterial smooth muscle. Jpn Cir J 38:1115–1119
Devynck MA, Pernollet MG, Nunez AM, Meyer P (1981) Calcium binding alteration in plasma membrane from various tissues of spontaneously hypertensive rats. Clin Exp Hyper 3:797–808
Limas CJ, Cohn JN (1977) Defective calcium transport by cardiac sarcoplasmic reticulum in spontaneously hypertensive rats. Cir Res 40, Suppl 1:62–69
Losse H, Zidek W, Zumkley H, Wessels F, Vetter H (1981) Intracellular Na+ as a genetic marker of essential hypertension. Clin Exp Hyper 3:627–640
Lowry OH, Rosebrough NJ, Farr AL, Randall RI (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Macintyre J, Breen JW (1978) Stimulation of calcium transport in inside-out vesicles of human erythrocyte membranes by a soluble cytoplasmic activator. Biochim Biophys Acta 510:373–377
Miledi R (1973) Transmitter release induced by injection of calcium in nerve terminals. Proc R Soc Lond Biol Sci 183:421–425
Nigli V, Adunyah ES, Penniston JT, Carafoli E (1981) Purified (Ca2+−M2+)-ATPase of the erythrocyte membrane. Reconstitution and effect of calmodulin and phospholipids. J Biol Chem 256:395–401
Orlov SN, Gulak PV, Karagodina ZV, Postnov YuV (1981) The properties of the erythrocyte membrane structure in spontaneous genetic hypertension. Cardiology (Moscow) 21, 7:108–112
Orlov SN, Postnov YuV (1980) Calcium accumulation and calcium binding by the cell membranes of cardiomyocytes and smooth muscle of aorta in spontaneously hypertensive rats. Clin Sci 59: 207s-209s
Postnov YuV, Orlov SN (1979) Features of intracellular calcium distribution in the adipose tissue of spontaneously hypertensive rats (SHR). Experientia 35:1480–1481
Postnov YuV, Orlov SN (1980) Evidence of altered calcium accumulation and calcium binding by the membranes of adipocytes in spontaneously hypertensive rats. Pflügers Arch 385:85–89
Postnov YuV, Orlov SN, Pokudin NI (1979) Decrease of calcium binding by the red blood cell membrane in spontaneously hypertensive rats and in essential hypertension. Pflügers Arch 379: 191–195
Postnov YuV, Orlov SN, Pokudin NI (1980) Alteration of intracellular calcium distribution in the adipose tissue of human patients with essential hypertension. Pflügers Arch 388:89–91
Postnov YuV, Orlov SN, Shevchenko AS (1977) Calcium ion binding and Na−K-ATPase activity in erythrocytes of patients with essential hypertension. Bull Exp Biol Med (Moscow) 84:7:41–44
Rasmussen H, Goodman DB (1978) Relation between calcium and cyclic nucleotides in cell activation. Physiol Rev 57:421–510
Romero RP (1976) Role of membrane bound Ca in ghost permeability to Na and K. J Membr Biol 29:329–343
Schatzmann H (1975) Active calcium transport across the plasma membrane of erythrocytes, In: Carafoli E, Clementi F, Drabikowski W, Magretti A (eds) Calcium transport in contraction and secretion. Amsterdam Oxford, pp 45–49
Steck TL (1974) Preparation of impermeable inside-out and right-side-out vesicles from erythrocyte membrane. In: Korn E (ed) Methods in membrane biology. Plenum Press, New York London, pp 245–281
Wei Wu-J, Janis RA, Daniel EE (1976) Studies of subcellular fraction from mesenteric arteries of spontaneously hypertensive rats: alterations in both calcium uptake and enzyme activities. Blood Vessels 13:293–308
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Orlov, S.N., Pokudin, N.I. & Postnov, Y.V. Calmodulin-dependent Ca2+ transport in erythrocytes of spontaneously hypertensive rats. Pflugers Arch. 397, 54–56 (1983). https://doi.org/10.1007/BF00585168
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DOI: https://doi.org/10.1007/BF00585168