Pflügers Archiv

, Volume 397, Issue 1, pp 20–24 | Cite as

Effects of concanavalin A on glutamate operated postsynaptic channels in crayfish muscle

  • H. Stettmeier
  • W. Finger
  • J. Dudel
Excitable Tissues and Central Nervous Physiology

Abstract

Small crayfish muscle fibres were voltage clamped, and synaptic current noise induced by bath application of glutamate was measured. Desensitization of the glutamate receptors was blocked by preincubating the fibres with 0.3–1.0 μmol/l concanavalin A (Con A) for at least 30 min. The power density spectra of the glutamate current noise could be fitted by single component Lorentz curves. The lectin Con A did not influence significantly the conductance γ of the glutamate channels but increased their mean open time, τnoise. The respective mean values found atT=8°C andE=−60 mV were γ=23.5±7.0 pS and τnoise=1.5 ±0.2 ms. Both the conductance γ and the closing rate α = τ noise −1 increased with temperature (Q10∼1.9). This temperature dependence was characterized by the activation energies Eγ=35.2±7.1 kJ/mol andEα=46.9±2.1 kJ/mol. The potential dependence of τnoise was almost completely abolished by Con A.

Key words

Glutamate induced synaptic current Excitatory synaptic channels Concanavalin A Desensitization Noise analysis 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • H. Stettmeier
    • 1
  • W. Finger
    • 1
  • J. Dudel
    • 1
  1. 1.Physiologisches Institut der Technischen Universität MünchenMünchen 40Germany

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