Skip to main content
SpringerLink
Log in

Effects of saline loading on jejunal absorption of calcium, sodium, and water, and on parathyroid hormone secretion in the rat

  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Summary

To investigate whether intestinal calcium absorption parallels that of sodium following extracellular fluid volume expansion, the effects of saline loading on intestinal transport of calcium. sodium and water were studied in rats by perfusing jejunal loops in situ.

After calcium-free saline infusion net calcium absorption was reversed similar to that of sodium and water and net secretion occurred. Concurrently, blood-to-lumen (b-l) calcium flux, measured using45Ca, increased significantly (P<0.001). Following expansion with calcium-containing Ringer a similar reversal of net calcium, sodium and water flux was also observed. Again, the b-l calcium flux increased but to a significantly lesser extent (P<0.05). Plasma ionized calcium remained unchanged after calcium-rich Ringer loading, but decreased significantly (P<0.001) when calcium was omitted from the solution. Plasma immunoreactive parathyroid hormone was unchanged after expansion with the calcium containing solution but increased following calcium-free infusion.

It is concluded that after extracellular fluid volume expansion: 1. net jejunal calcium absorption is decreased; 2. the decrease parallels that of sodium and water; 3. b-l calcium transport is enhanced to a greater degree by calcium-free Ringer infusion than by a calcium-rich solution. This difference could be the result of increased parathyroid hormone secretion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Agus, Z. S., Gardner, L. B., Beck, L. H., Goldberg, M.: Effects of parathyroid hormone on renal tubular reabsorption of calcium, sodium, and phosphate. Amer. J. Physiol.224, 1143–1148 (1973)

    Google Scholar 

  2. Arnaud, C. D., Tsao, H. S., Littledike, T.: Radio-immunoassay of human parathyroid hormone in serum. J. clin. Invest.50, 21–34 (1971)

    Google Scholar 

  3. Bader, C., Assailly, J., Chanard, J., Funck-Brentano, J. L.: Comptage direct des échantillons plasmatiques en scintillation liquide par étalonnage interne-externe. Int. J. appl. Rad. Isotopes24, 29–36 (1973)

    Google Scholar 

  4. Birge, S. J., Switzer, S. C., Leonard, D. R.: Influence of sodium and parathyroid hormone on calcium release from intestinal mucosa cells. J. clin. Invest.54, 702–709 (1974)

    Google Scholar 

  5. Blythe, W. B., Gitelman, H. J., Welt, L. G.: Effect of expansion of the extracellular space on the rate of urinary excretion of calcium. Amer. J. Physiol.214, 52–57 (1968)

    Google Scholar 

  6. Bonjour, J. P., Malvin, R. L.: Plasma concentrations of ADH in conscious and anesthetized dogs. Amer. J. Physiol.218, 1128–1132 (1970)

    Google Scholar 

  7. Borle, A. B.: Calcium and phosphate metabolism. Ann. Rev. Physiol.36, 361–390 (1974)

    Google Scholar 

  8. Boulpaep, E. L.: Permeability changes of the proximal tubule of necturus during saline loading. Amer. J. Physiol.222, 517–531 (1972)

    Google Scholar 

  9. Brenner, B. M., Troy, J. L., Daugharty, T. M.: On the mechanism of inhibition in fluid reabsorption by the renal proximal tubule of the volume-expanded rat. J. clin. Invest.50, 1596–1602 (1971)

    Google Scholar 

  10. Charney, A. N., Kinsey, M. D., Myers, L., Gianella, R. A., Gots, R. E.: Na+-K+-activated adenosine triphosphatase and intestinal electrolyte transport. Effect of adrenal steroids. J. clin. Invest.56, 653–660 (1975)

    Google Scholar 

  11. Cortney, M. A., Mylle, M., Lassiter, W. E., Gottschalk, C. W.: Renal tubular transport of water, solute and PAH in rats loaded with isotonic saline. Amer. J. Physiol.209, 1199–1205 (1965)

    Google Scholar 

  12. Cremaschi, D., Galante, M.: Action of posthypophyseal hormones on the “in vivo” isosmotic net water transport and adenylcyclase in rabbit gall bladder. Arch. intern. Physiol. Biochim.77, 819–828 (1969)

    Google Scholar 

  13. Crocker, A. D., Munday, K. A.: Factors affecting mucosal water and sodium transfer in everted sacs of rat jejunum. J. Physiol. (Lond.)202, 329–338 (1969)

    Google Scholar 

  14. De Luca, H.: Recent advances in our understanding of the vitamin D endocrine system. J. Lab. clin. Med.87, 7–26 (1976)

    Google Scholar 

  15. Dennhardt, R., Haberich, F. J.: Effect of ADH on the net transport of water and electrolytes in the small bowel of anesthetized rats. Pflügers Arch.334, 74–84 (1972)

    Google Scholar 

  16. Dirks, J. H., Cirksena, W. J., Berliner, R. W.: The effect of saline infusion on sodium reabsorption by proximal tubule of dog. J. clin. Invest.44, 1160–1170 (1965)

    Google Scholar 

  17. Dunn, F. L., Brennan, T. J., Nelson, A. E., Robertson, G. L.: The role of blood osmolality and volume in regulating vasopressin secretion in the rat. J. clin. Invest.52, 3212–3219 (1973)

    Google Scholar 

  18. Fitzgibbons, J. P., Gennari, F. J., Garfinkel, H. B., Cortell, S.: Dependence of saline-induced natriuresis upon exposure of the kidney to the physical effects of extracellular fluid volume expansion. J. clin. Invest.54, 1428–1436 (1974)

    Google Scholar 

  19. Frick, A.: Mechanism of inorganic phosphate diuresis secondary to saline infusions in the rat. Pflügers Arch.313, 106–122 (1969)

    Google Scholar 

  20. Grandchamp, A., Boulpaep, E. L.: Pressure control of sodium reabsorption and intracellular backflux across proximal kidney tubule. J. clin. Invest.54, 69–82 (1974)

    Google Scholar 

  21. Green, R., Windhager, E. E., Giebisch, G.: Protein oncotic pressure effects on proximal tubular fluid movement in the rat. Amer. J. Physiol.226, 265–276 (1974)

    Google Scholar 

  22. Gutman, Y., Benzakein, F.: Effect of saline loading on absorption from cat ileum in vivo. Israel J. med. Sci.6, 195–200 (1970)

    Google Scholar 

  23. Hakim, A. A., Lifson, N.: Effects of pressure on water and solute transport by dog intestinal mucosa in vitro. Amer. J. Physiol.216, 276–284 (1969)

    Google Scholar 

  24. Hays, R. M.: Antidiuretic hormone and water transfer. Kidney Intern.9, 223–230 (1976)

    Google Scholar 

  25. Hebert, L. A., Stuart, K. A., Stemper, J. A.: Renal expansion in saline loading. Amer. J. Physiol.227, 406–411 (1974)

    Google Scholar 

  26. Higgins, J. T., Blair, N. P.: Intestinal transport of water and electrolytes during extracellular volume expansion in dogs. J. clin. Invest.50, 2569–2579 (1971)

    Google Scholar 

  27. Humphreys, M. H., Early, L. E.: The mechanisms of decreased intestinal sodium and water absorption after acute volume expansion in the rat. J. clin. Invest.50, 2355–2367 (1971)

    Google Scholar 

  28. Ireland, P., Fordtran, J. S.: Effect of dietary calcium and age on jejunal calcium absorption in humans studied by intestinal perfusion. J. clin. Invest.52, 2672–2681 (1973)

    Google Scholar 

  29. Knox, F. G., Schneider, E. G., Willis, L. R., Strandhoy, J. W., Ott, C. E., Cuche, J. L., Goldsmith, R. S., Arnaud, C. D.: Proximal tubule reabsorption after hyperoncotic albumin infusion. J. clin. Invest.53, 501–507 (1974)

    Google Scholar 

  30. Lee, J. S.: Effects of pressure on water absorption and secretion in rat jejunum. Amer. J. Physiol.224, 1338–1344 (1973)

    Google Scholar 

  31. Martino, J. A., Early, L. E.: Relationship between intrarenal hydrostatic pressure and hemodynamically induced changes in sodium excretion. Circulat. Res.23, 371–386 (1968)

    Google Scholar 

  32. Massry, S. G., Coburn, J. W., Chapman, L. W., Kleeman, C. R.: Effect of NaCl infusion on urinary Ca and Mg during reduction in their filtered loads. Amer. J. Physiol.213, 1218–1224 (1967)

    Google Scholar 

  33. McLeod, G. M., French, A. B., Good, C. J., Wright, F. S.: Gastrointestinal absorption and biliary excretion of phenolsulfonphtalein (phenol red) in man. J. Lab. clin. Med.71, 192–200 (1968)

    Google Scholar 

  34. Nellans, H. N., Frizzel, R. A., Schultz, S. G.: Brush border processes and transepithelial Na and Cl transport by rabbit ileum. Amer. J. Physiol.226, 1131–1141 (1974)

    Google Scholar 

  35. Nizet, A., Jost, H., Foidart-Willems, J.: The control of sodium excretion following saline infusion in dogs. Pflügers Arch.350, 287–298 (1974)

    Google Scholar 

  36. Nizet, A., Robin, M., Merchie, G.: Renal control of intestinal sodium and water transport in the dog. Pflügers Arch.364, 59–64 (1976)

    Google Scholar 

  37. Ott, C. E., Haas, J. A., Cuche, J. L., Knox, F. G.: Effect of increased peritubule protein concentration on proximal tubule reabsorption in the presence and absence of extracellular volume expansion. J. clin. Invest.55, 612–620 (1975)

    Google Scholar 

  38. Rector, F. C., Martinez-Maldonado, M., Kurtzmann, N. A., Sellman, J. C., Derther, F., Seldin, D. W.: Demonstration of a hormonal inhibitor of proximal tubular reabsorption expansion of extracellular volume with isotonic saline. J. clin. Invest.47, 761–773 (1968)

    Google Scholar 

  39. Richet, G., Hornych, A.: The effect of an expansion of extracellular fluid on net sodium flux in the jejunum of rats. Nephron6, 365–378 (1969)

    Google Scholar 

  40. Schultz, S. G., Frizzel, R. A., Nellans, H. N.: Ion transport by mammalian small intestine. Ann. Rev. Physiol.36, 51–88 (1974)

    Google Scholar 

  41. Ussing, H. H., Erly, D., Lassen, U.: Transport pathways in biological membranes. Ann. Rev. Physiol.36, 17–49 (1974)

    Google Scholar 

  42. Vatner, S. F.: Effects of hemorrhage on regional blood flow distribution in dogs and primate. J. clin. Invest.54, 225–235 (1974)

    Google Scholar 

  43. Walser, M.: Calcium clearance as a function of sodium clearance in the dog. Amer. J. Physiol.200, 1099–1104 (1961)

    Google Scholar 

  44. Walton, J., Williams, M. E., Shea, T., Gray, T. K.: Effects of parathyroid extract on jejunal absorption of phosphate and calcium. Clin. Res.24, 30A (1976)

    Google Scholar 

  45. De Wardener, H. E., Mills, I. H., Clapham, W. F., Hayter, C. J.: Studies on the efferent mechanism of sodium diuresis which follows administration of intravenous saline in the dogs. Clin. Sci.21, 249–258 (1961)

    Google Scholar 

  46. Younoszai, M. K., Urban, E., Schedl, H. P.: Vitamin D and intestinal calcium fluxes in vivo in the rat. Amer. J. Physiol.225, 287–292 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Département de Néphrologie, I.N.S.E.R.M.U. 90, Hôpital Necker, Université de Paris V, 161, rue de Sèvres, F-75730, Paris-Cedex 15, France

    Jacques Chanard, Tilman Drüeke, Eric Pujade-Lauraine, Bernard Lacour, Jean-Louis Funck-Brentano & D. Coraboeuf

Authors
  1. Jacques Chanard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tilman Drüeke
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eric Pujade-Lauraine
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bernard Lacour
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jean-Louis Funck-Brentano
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. Coraboeuf
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chanard, J., Drüeke, T., Pujade-Lauraine, E. et al. Effects of saline loading on jejunal absorption of calcium, sodium, and water, and on parathyroid hormone secretion in the rat. Pflugers Arch. 367, 169–175 (1976). https://doi.org/10.1007/BF00585154

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00585154

Key words

Search

Navigation