Abstract
The transepithelial permeability properties to Na, K, and Cl in microperfused segments of human eccrine sweat ducts from normal (N) subjects and patients with cystic fibrosis (CF) were examined. Amiloride administered on the luminal surface caused the transepithelial potential (V t ) of normal ducts to depolarize to 0 mV, but in the absence of Cl in the medium or in CF ducts, amiloride caused theV t to significantly reverse electrical polarity from lumen negative to lumen positive with respect to the serosal bath. TheV t responses to changes in Na concentration in the lumen and K concentration in the bath were similar in CF and N ducts and showed that the basolateral membrane of the duct is K permeable and the apical membrane (in the absence of an anion shunt) is an almost ideal Na electrode. TheV t of N ducts was insensitive to 10-fold changes in luminal K and contraluminal Na solution concentrations. These responses show that in normal ducts, the apical membrane and tight junctions are relatively impermeable to K, and the basal membrane and tight junctions are relatively impermeable to Na. TheV t was highly sensitive to Cl− changes on either surface before or after ouabain inhibition in N ducts, but in every case were insensitive to Cl− changes in CF ducts. By comparison to control ducts the cation selective properties of the CF duct are probably normal, but both cell membranes as well as the tight junctions of the CF duct are relatively impermeable to Cl. The present data are inconclusive as to whether the route of Cl movement across the N duct epithelium is trans- or paracellular.
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Al-Awqati Q, Gluck S, Reeves, W, Cannon C (1983) Regulation of proton transport in urinary epithelia. J Exp Biol 106:135–141
Bijman J, Quinton PM (1984A) Influence of abnormal Cl permeability on sweating in cystic fibrosis. Am J Physiol 247:C3-C9
Bijman J, Quinton PM (1984b) Apparent absence of cystic fibrosis sweat factor on ion-selective and transport properties of the perfused human sweat duct. Ped Res 18:1292–1296
Bijman J, Quinton PM (1987) Lactate and bicarbonate uptake in the sweat duct of cystic fibrosis and normal subjects. Ped Res 21:79–82
Bijman J, Lang HJ, Englert HC, Greger R, Frömter E (1985) Cl−-conductance of the microperfused sweat duct, inhibition by 3,4-dichlorodiphenyl-2-carboxylase (DDCP). Pflügers Arch 405:R26
Cuthbert AW, Fanelii GM Jr, Scriabine A (eds) (1979) Amiloride an epithelial sodium transport. Urban and Schwarzenberg, Baltimore
Frizell RA, Rechkemmer G, Shoemaker RL (1986) Altered regulation of airway epithelial cell chloride channels in cystic fibrosis. Science 233:560
Gatzy JT, Clarkson TW (1965) The effect of mucusal and serosal solution cations on bioelectric properties of the isolated toad bladder. J Gen Physiol 48:647
Giff ER, Steinberg RH (1984) Changes in apical [K+] produced delayed basal membrane responses in the retinal pigment epithelium in the gecko. J Gen Physiol 48:193–211
Goldman DE (1943) Potential, impedance, and rectification in membranes. J Gen Physiol 27:37–60
Jones CJ, Kealey T (1986) Electrophysiological studies on isolated human eccrine sweat glands. Q J Exp Physiol 71:123–132
Kaiser D, Williams RS, Drack E (1974) Hydrogen ion and electrolyte excretion of the single human sweat gland. Pflügers Arch 349:63–72
Knowles MR, Stutts MJ, Spock A, Fisher N, Gatzy JT, Boucher RC (1983) Abnormal ion permeation through cystic fibrosis respiratory epithelium. Science 221:1067–1070
Koeford-Johnson V, Ussing HH (1958) The nature of the frog skin potential. Acta Physiol Scand 42:298–308
Lewis SA, Hanrahan JW (1985) Apical and basolateral membrane ionic channels in rabbit urinary bladder epithelium. Pflügers Arch 405:583–588
Nikolajek WP, Emrich HM (1976) pH of sweat of patients with cystic fibrosis. Klin Wschr 54:287–288
Quinton PM (1981) Effects of some transport inhibitors on secretion and reabsorption in intact and perfused single human sweat glands. Pflügers Arch 391:309–373
Quinton PM (1983) Chloride impermeability in cystic fibrosis. Nature 301:421–422
Quinton PM (1986) Missing Cl conductance in cystic fibrosis. Am J Physiol 221:C649-C652
Quinton PM, Bijman J (1983) Higher bioelectric potentials in sweat glands due to decrease Cl absorption in patients with cystic fibrosis. New England J Med 308:185–189
Sato K (1982) Mechanisms of eccrine sweat secretion. In: Quinton PM, Martinez JR, Hopfer U (eds) Fluid and electrolyte abnormalities in exocrine glands in cystic fibrosis. San Francisco Press Inc, San Francisco, pp 35–52
Schulz IJ (1969) Micropuncture studies of the sweat formation in cystic fibrosis patients. J Clin Invest 48:1470–1477
Welsh M, Liedtke CM (1986) Chloride and potassium channels in cystic fibrosis (CF) airway epithelia. Nature 322:467–470
Widdicombe JH, Welsh MJ, Finkbeiner WE (1985) Cystic fibrosis decreases the apical membrane chloride permeability of monolayers cultured from cells of tracheal epithelium. Proc Natl Acad Sci USA 82:6167–6171
Yankaskas J, Cotton CU, Knowles MR, Gatzy JT, Boucher RC (1985) Culture of human nasal epithelial cells on collagen matrix supports. Am Rev Res Dis 132:1281–1287
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Bijman, J., Quinton, P. Permeability properties of cell membranes and tight junctions of normal and cystic fibrosis sweat ducts. Pflugers Arch. 408, 505–510 (1987). https://doi.org/10.1007/BF00585076
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DOI: https://doi.org/10.1007/BF00585076