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Evidence for rheogenic sodium bicarbonate cotransport in the basolateral membrane of oxyntic cells of frog gastric fundus

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

Ionic conductance properties of the basolateral cell membrane of oxyntic cells were studied in frog gastric fundus in vitro. After mounting the fundus in a modified Ussing chamber the serosal connective tissue was dissected off and individual oxyntic cells were punctured from the serosal surface with microelectrodes. Under resting conditions the membrane potential averaged −56.9, SD±9.5 mV (n=63), cytoplasm negative. Lowering or raising serosal HCO −3 concentration from 17.8 to 6 or 36 mmol/l respectively at constant\(p_{CO_2 } \) depolarized or hyperpolarized the cell membrane by +16.7 or −18.2 mV respectively. Sudden removal of serosal Na+ also depolarized the cell membrane (anomalous Nernst response). Since both the HCO −3 dependent and the Na+ dependent potential changes were strongly depressed by the disulfonic stilbene SITS and since the potential response to HCO −3 was virtually abolished in Na+-free solution we conclude that a rheogenic Na+ (HCO −3 ) n -cotransport system (n>1) is present in the basolateral cell membrane of oxyntic cells. Its possible role in base transfer during HCl-secretion or HCO −3 secretion remains to be elucidated.

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Authors and Affiliations

  1. Istituto di Fisiologia Generale, Universita di Bari, Via Amendola 165 A, I-70126, Bari, Italy

    S. Curci

  2. Zentrum der Physiologie, J. W. Goethe-Universität, Theodor-Stern-Kai 7, D-6000, Frankfurt 70, Germany

    L. Debellis & E. Frömter

Authors
  1. S. Curci
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  2. L. Debellis
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  3. E. Frömter
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Additional information

This work was supported by grants Nr. 85.0443.04 and 86.00048.04 from Consiglio Nazionale delle Ricerche, Rome, Italy and by grant Nr. I 37736 from Stiftung Volkswagenwerk, Hannover, FRG

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Curci, S., Debellis, L. & Frömter, E. Evidence for rheogenic sodium bicarbonate cotransport in the basolateral membrane of oxyntic cells of frog gastric fundus. Pflugers Arch. 408, 497–504 (1987). https://doi.org/10.1007/BF00585075

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  • DOI: https://doi.org/10.1007/BF00585075

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