Pflügers Archiv

, Volume 408, Issue 5, pp 479–483 | Cite as

Effects of glutaraldehyde fixation on renal tubular function

I. Preservation of vasopressin-stimulated water and urea pathways in rat papillary collecting duct
  • Yoshiaki Kondo
  • Masashi Imai
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


Using the in vitro microperfusion technique on isolated rat papillary collecting duct (PCD), we examined whether the glutaraldehyde-fixation method can be also applied to the mammalian collecting duct for preservation of the vasopressin-stimulated water and urea transport. Arginine vasopressin (AVP) at 10−9 mol/l increased diffusional water permeability (Pdw) from 101.9±10.76 to 283.3±16.67×10−7 cm2 s−1 (n=8,P<0.01) and urea permeability (Purea) from 30.3±2.24 to 83.5±7.80×10−7 cm2 s−1 (n=8,P<0.01). Both parameters remained elevated after fixation with 0.1 mol/l glutaraldehyde even in the absence of AVP, with the values being 265.0±14.47 and 74.5±7.15×10−7 cm2 s−1, respectively. Glutaraldehyde fixation did not affect the basal levels ofPdw orPurea. Phloretin at 2.5×10−4 mol/l decreased glutaraldehyde-fixed AVP-stimulatedPurea from 79.0±7.96 to 29.7±3.66×10−7 cm2 s−1 (n=4,P<0.01) and from 73.2±7.05 to 38.7±3.53×10−7 cm2 s−1 (n=4,P<0.01) when the drug was added to the lumen or to the bath, respectively. Phloretin also decreased glutaraldehyde-fixed non-stimulatedPurea by 25–40%. However, this drug did not affect glutaraldehyde-fixedPdw. These findings indicate that the glutaraldehyde fixation method can be applied to mammalian collecting tubules for studying vasopressin stimulatedPdw andPurea.Purea fixed by glutaraldehyde is functionally flexible and may be distinct from the water pathway.

Key words

Papillary collecting duct Vasopressin Phloretin Water channel Urea channel Glutaraldehyde 


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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Yoshiaki Kondo
    • 1
  • Masashi Imai
    • 1
  1. 1.Department of PharmacologyNational Cardiovascular Center, Research InstituteSuita, OsakaJapan

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