Abstract
PGE2 synthesis was measured along the nephron of Brattleboro (DI) rats, lacking ADH, and control LE rats, using an enzyme immunossay. Experiments were performed in vitro, in the absence of exogenous arachidonic acid, using microdissected tubular segments. The effect of a chronic treatment by dDAVP was tested on three ADH sensitive tubular segments, medullary thick ascending limb (MTAL), medullary collecting tubule (OMCD) and papillary collecting duct (IMCD).
No difference in PGE2 synthesis was present between LE and DI in glomerulus and tubular segments up to OMCD. In both strains, values were low in the proximal tubule and the loop of Henle, and gradually increased along the collecting tubule. In IMCD, PGE2 synthesis was much higher in DI (12.8±2.0 pg per 30 min per mm tubular length) than in LE (3.8±0.5, LE vs. DIp<0.001).
In MTAL and OMCD, dDAVP treatment did not affect PGE2 synthesis. In IMCD, dDAVP reduced PGE2 synthesis to values (5.3±0.8 pg per 30 min per mm tubular length), which were not significantly different from those of LE. Neither oxytocin, which has been shown to be elevated in DI rats, nor furosemide, that reduced papillary osmolarity to values comparable to those of DI rats, were able to increase PGE2 synthesis in IMCD of LE rats. The mechanism of the increase in PGE2 synthesis in IMCD of DI rats, and of the inhibitory effect of dDAVP is yet unknown; it may participate to compensate for the lack of ADH in the Brattleboro rat.
Similar content being viewed by others
References
Abdel-Latif AA (1986) Calcium-mobilizing receptors, polyphosphoinositides and the generation of second messengers. Pharmacol Rev 38:227–272
Bankir L, Trinh-Trang-Tan MM, Nivez MP, Sraer J, Ardaillou R (1980) Altered PGE2 production by glomeruli and papilla of rats with hereditary diabetes insipidus. Prostaglandins 20:349–365
Berridge MJ (1986) Cell signalling through phospholipid metabolism. J Cell Sci 4:137–153
Bonvalet JP, Pradelles P, Farman N (1987) Segmental synthesis and actions of prostaglandins along the nephron. Am J Physiol 253:F377-F387
Bouby N, Bankir L, Trinh-Trang-Tan MM (1985) Selective ADH-induced hypertrophy of the medullary thick ascending limb in Brattleboro rats. Kidney Int 28:456–466
Craven PA, DeRubertis FR (1981) Effects of vasopressin and urea on Ca2+-calmodulin-dependent renal prostaglandin E. Am J Physiol 241:F649-F658
Craven PA, Briggs R, DeRubertis FR (1980) Calcium-dependent action of osmolarity on adenosine 3′,5′-monophosphate accumulation in rat renal inner medulla. J Clin Invest 65: 529–542
Danon A, Knapp HR, Oelz O, Oates JA (1978) Stimulation of prostaglandin biosynthesis in the renal papilla by hypertonic mediums. Am J Physiol 234:F64-F67
Dunn MJ, Beck TR, Kinter LB, Hassid A (1983) The effects of vasopressin and vasopressin analogs upon renal synthesis of prostaglandins. In: Dunn MJ, Patrono C, Cinotti GA (eds) Prostaglandins and the kidney. Plenum Press, New York, pp 151–166
Edwards BR, LaRochelle FT Jr, Gellai M (1982) Concentration of urine by dehydrated Brattleboro homozygotes: is there a role for oxytocin? Ann N Y Acad Sci 394:497–502
Farman N, Pradelles P, Bonvalet JP (1986) Determination of prostaglandin E2 synthesis along the rabbit nephron by enzyme immunoassay. Am J Physiol 251:F238-F244
Farman N, Pradelles P, Bonvalet JP (1987) PGE2, PGF2α, 6-keto-PGF1α, and TXB2 synthesis along the rabbit nephron. Am J Physiol 252:F53-F59
Garcia-Perez A, Smith WL (1984) Apical-basolateral membrane asymetry in canine cortical collecting tubule cells. J Clin Invest 74:63–74
Hassid A, Konieczkowski M, Dunn MJ (1979) Prostaglandin synthesis in isolated rat kidney glomeruli. Proc Natl Acad Sci 76:1155–1159
Imbert-Teboul M, Siaume S, Morel F (1986) Sites of prostaglandin E2(PGE2) synthesis along the rabbit nephron. Mol Cell Endocrinol 45:1–10
Jackson BA (1986) Prostaglandin E2 synthesis in the inner medullary collecting duct of the rat: implications for vasopressin-dependent cyclic AMP formation. J Cell Physiol 129:60–64
Jackson BA (1986) Renal prostaglandin E2 synthesis in the Brattleboro homozygous diabetes insipidus rat. Prostaglandins Leukotrienes Med 22:101–110
Kirschenbaum MA, Lowe AG, Trizna W, Fine LG (1982) Regulation of vasopressin action by prostaglandins. Evidence for prostaglandin synthesis in the rabbit cortical collecting tubule. J Clin Invest 70:1193–1204
Kokko JP (1987) The role of collecting duct in urinary concentration. Kidney Int 31:606–610
Nonoguchi H, Uchida S, Shiigai T, Endou H (1985) Effect of chronic metabolic acidosis on ammonia production froml-glutamine in microdissected rat nephron segments. Pflügers Arch 403:229–235
North WG, Gellai M, Hardy G (1982) Oxytocin and oxytocin-associated neurophysin evaluation by RIA in the Brattleboro rat: turnover. Ann N Y Acad Sci 394:167–172
Portilla D, Shayman JA, Morrison AR (1987) Vasopressin does not hydrolyze polyphosphoinositides in rabbit papillary collecting tubule cells. Biochim Biophys Acta 928:305–311
Sands JM, Nonoguchi H, Knepper M (1987) Vasopressin effects on urea and H2O transport in inner medullary collecting duct subsegments. Am J Physiol 253:F823-F832
Sato WH Dunn MJ (1986) Osmolality, vasopressin-stimulated cAMP, and PGE2 synthesis in rat collecting tubule cells. Am J Physiol 250:F802-F810
Sawyer WH, Agosta M, Balspiri L, Judd J, Manning M (1974) Structural changes in the arginine vasopressin molecule that enhance antidiuretic activity and specificity. Endocrinology 94:1106–1115
Schlondorff D, Ardaillou R (1986) Prostaglandins and other arachidonic acid metabolites in the kidney. Kidney Int 29: 108–119
Schlondorff D, Roczniak S, Satriano JA, Folkert VW (1980) Prostaglandin synthesis by isolated rat glomeruli: effect of angiotensin II. Am J Physiol 238:F486-F495
Schlondorff D, Satriano JA, Schwartz GS (1985) Synthesis of prostaglandin PGE2 in different segments of isolated collecting tubules from adult and neonatal rabbits. Am J Physiol 248:F134-F144
Smith WL (1981) Renal prostaglandin biochemistry. Miner Electrolyte Metab 6:10–26
Sraer J, Sraer JD, Chansel D, Russo-Marie F, Kouznetzova B, Ardaillou R (1979) Prostaglandin synthesis by isolated rat renal glomeruli. Mol Cell Endocrinol 16:29–37
Torikai S (1987) Tubular prostaglandin E2 production and its role in urinary hypotonicity after release of ureteral occlusion in the rat. Clin Sci 73:395–399
Wuthrich RP, Loup R, Favre L, Valloton MB (1986) Dynamic response of PG synthesis to peptide hormones and osmolality in renal tubular cells. Am J Physiol 250:F790-F797
Zusman RM, Keiser HR (1980) Regulation of prostaglandin E2 synthesis by angiotensin II, potassium, osmolality, and dexamethasone. Kidney Int 17:277–283
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jaisser, F., Blot-Chabaud, M., Pradelles, P. et al. Antidiuretic hormone reduces the high PGE2 synthesis in papillary collecting duct of DI rats. Pflugers Arch. 414, 464–468 (1989). https://doi.org/10.1007/BF00585058
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00585058