Pflügers Archiv

, Volume 395, Issue 1, pp 55–58 | Cite as

Batrachotoxin protects sodium channels from the blocking action of oenanthotoxin

  • J. M. Dubois
  • B. I. Khodorov
Excitable Tissues and Central Nervous Physiology


  1. 1.

    The blocking action of oenanthotoxin (OETX) and butanol on Na+ channels was studied in voltage clamp experiments on single myelinated nerve fibres treated by batrachotoxin (BTX).

  2. 2.

    OETX (40 μM) blocked Na+ currents through normal channels but did not affect significantly the BTX modified Na+ current.

  3. 3.

    BTX removed the depolarization-induced charge immobilization and slowed down significantly the OFF charge movement. However, the maximum charge displaced, as well as the kinetics of the ON charge movement during a strong membrane depolarization, remained unchanged.

  4. 4.

    OETX blocked the charge movement in normal Na+ channels but did not affect noticeably the charge movement modified by BTX.

  5. 5.

    BTX did not modify the K+ currents and did not protect them from the blocking action of OETX.

  6. 6.

    Butanol (0.01–0.1 M) decreased almost identically and reversibly both normal and BTX-modified Na+ currents.

  7. 7.

    It is concluded that binding of BTX to its receptor protects the Na+ channel from interaction with OETX but left it accessible to butanol.


Key words

Nerve membrane Na+ channel Batrachotoxin Oenanthotoxin Butanol 


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • J. M. Dubois
    • 1
  • B. I. Khodorov
    • 2
  1. 1.Laboratoire de neurobiologieEcole Normale SupérieureParisFrance
  2. 2.A. V. Vishnevsky Surgery InstituteMoscowUSSR

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