Pflügers Archiv

, Volume 395, Issue 1, pp 49–54 | Cite as

Effects of Ca antagonist on the contractile force in glycerinated dog heart muscles

  • Yokio Maruyama
  • Hiroshi Okayama
  • Nobumasa Ishide
  • Tamotsu Takishima
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology


The effect of Ca antagonist on the contractile apparatus was investigated in glycerinated cardiac muscle preparations obtained from canine hearts. Each muscle preparation had three consecutive isometric contractions. The 1st and 3rd contractions were produced with a control contraction solution, and compared with the 2nd contraction which was induced with a contraction solution containing verapamil. The results showed that maximal developed tension (Po) was enhanced significantly by 1.02×10−2 mM of verapamil, and the augmentation of contractility was dependent on the concentrations of verapamil. Thus, not only Po, but also dT/dt increased tremendously at 1.02 mM of verapamil. Such contractile potentiation by verapamil was also ascertained by another Ca antagonist, Diltiazem hydrochloride. The developed tension was maximum at pCa 4.0, and no developed tension was found at pCa 8.0. The relationship between pCa and tension with verapamil shifted to the left from that without verapamil, showing higher sensitivity to Ca2+. From these results, it was strongly indicated that Ca antagonist is a potentiating agent of the contractile force.

Key words

Ca-antagonist Contractility Glycerinated heart muscle fibers Verapamil Diltiazem 


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  1. Briggs FN, Fuchs F (1963) The nature of the muscle-relaxing factor. I. An improved assay system. J Gen Physiol 46:883–891Google Scholar
  2. Cherney KJ, Bohr DF, Murphy RA (1971) Potentiation of contraction of glycerol-extracted muscle by deoxycholate. Proc Soc Exp Biol Med 136:141–145Google Scholar
  3. Fleckenstein A (1971) Specific inhibitors and promoters of calcium action in excitation-contraction coupling of heart muscle and their role in prevention of production of myocardial lesions. In: Harris P, Opie L (eds) Calcium and the heart. Academic Press, New York, pp 135–188Google Scholar
  4. Fossard HA (1977) Heart. Excitation-contraction coupling. Annu Rev Physiol 39:201–220Google Scholar
  5. Godt RE (1974) Calcium-activated tension of skinned muscle fibers of the frog. Dependence of magnesium adenosine triphosphate concentration. J Gen Physiol 63:722–739Google Scholar
  6. Halloway JH, Reilley CN (1960) Metal chelate stability constants of aminopolycarboxylate ligands. Anal Chem 32:249–256Google Scholar
  7. Henry PD, Ahumada GG, Friedman WF, Sobel BE (1972) Simultaneously measured isometric tension and ATP hydrolysis in glycerinated fibers from normal and hypertrophied rabbit heart. Circ Res 31:740–746Google Scholar
  8. Himori N, Ono H, Taira N (1976) Simultaneous assessment of effects of coronary vasodilators on the coronary blood flow and the myocardial contractility by using the blood-perfused canine papillary muscle. Jpn J Pharmacol 26:427–435Google Scholar
  9. Infante AA, Klaupiks D, Davies RE (1964) Length, tension and metabolism during short isometric contractions of frog sartorius muscles. Biochim Biophys Acta 88:215–217Google Scholar
  10. Katz AM (1970) Contractile proteins of the heart. Physiol Rev 50:63–158Google Scholar
  11. Kohlhardt M, Bauer B, Krause H, Fleckenstein A (1972) Differentiation of the transmembrane Na and Ca channels in mammalian cardiac fibers by the use of specific inhibitors. Pflügers Arch 335:309–322Google Scholar
  12. Kreye VAW, Lüth JB (1975) Excitation of the rat vas deferens by verapamil and local anaesthetics. Pflügers Arch 355 (Suppl):R 59Google Scholar
  13. Martonosi A (1968) Sarcoplasmic reticulum. IV. Solubilization of microsomal adenosine triphosphatase. J Biol Chem 243:71–81Google Scholar
  14. Maruyama Y, Bing RJ, Sarma JSM, Weishaar R (1978) The effect of alcohol on active and passive stiffness and isometric contractions of glycerinated heart muscle in rats. Jpn Heart J 19:513–521Google Scholar
  15. Maruyama Y, Ishide N, Okayama H, Ino-Okada E, Takishima T (1979) Effects of verapamil on the contractile force in the glycerinated dog heart muscles. Tohoku J Exp Med 128:199–200Google Scholar
  16. Maruyama Y, Fischer R, Bing RJ (1980) The effect of regional myocardial ischemia on series elastic and contractile elements of glycerinated heart muscle in dogs. Jpn Circ J 44:449–460Google Scholar
  17. Nayler WG, Szeto J (1972) Effect of verapamil on contractility, oxygen utilization and calcium exchangeability in mammalian heart muscle. Cardiovasc Res 6:120–128Google Scholar
  18. Philips RC, George P, Rutman RJ (1966) Thermodynamic studies of the formation and ionization of the magnesium. II. Complexes of ADP and ATP over the pH range 5 to 9. J Am Chem Soc 88:2631–2640Google Scholar
  19. Sarma JSM, Ikeda S, Fischer R, Maruyama Y, Weishaar R, Bing RJ (1976) Biochemical and contractile properties of heart muscle after prolonged alcohol administration. J Mol Cell Cardiol 8:951–972Google Scholar
  20. Schwartzenbach G, Senn H, Anderegg H (1957) Komplexone. XXIX. Ein grosser Chelateffekt besonderer Art. Helv Chim Acta 40:1886–1900Google Scholar
  21. Storer AC, Cornish-Bowden A (1976) Concentration of Mg ATP2− and other ions in solution. Calculation of the true concentrations of species present in mixtures of associating ions. Biochem J 159:1–5Google Scholar
  22. Suarez-Kurtz G, Sorenson AL (1977) Effects of verapamil on excitation-contraction coupling in single crab muscle fibers. Pflügers Arch 368:231–239Google Scholar
  23. Szent-Gyorgyi A (1949) Free energy relations and contractions of actomyosin. Biol Bull 96:140–161Google Scholar
  24. Watanabe AM, Besch HR Jr (1974) Subcellular myocardial effects of verapamil and D600: comparison with propranolol. J Pharmacol Exp Ther 191:241–251Google Scholar
  25. Weishaar R, Sarma JSM, Maruyama Y, Fischer R, Bing RJ (1977a) Regional blood flow, contractility and metabolism in early myocardial infarction. Cardiology 62:2–20Google Scholar
  26. Weishaar R, Sarma JSM, Maruyama Y, Fischer R, Bertuglia S, Bing RJ (1977b) Reversibility of mitochondrial and contractile changes in the myocardium after cessation of prolonged ethanol intake. Am J Cardiol 40:556–562Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Yokio Maruyama
    • 1
  • Hiroshi Okayama
    • 1
  • Nobumasa Ishide
    • 1
  • Tamotsu Takishima
    • 1
  1. 1.First Department of Internal MedicineTohoku University School of MedicineSendaiJapan

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