Abstract
The Bohr factor, φ=Δ log\(P_{{\text{O}}_2 }\)/ΔpH, was determined at various levels of hemoglobin O2 saturation (\({\text{S}}_{{\text{O}}_{\text{2}} }\)) in fresh whole blood of the duck. Plasma pH was varied by either changing\(P_{{\text{CO}}_2 }\) of the blood at constant base excess (CO2 Bohr factor,\(\varphi _{{\text{C0}}_2 }\)) or by addition of NaHCO3 and HCl at constant\(P_{{\text{CO}}_2 }\) (fixed acid Bohr factor, ϕAH). No differences were found between\(\varphi _{{\text{C0}}_2 }\) and ϕAH at\({\text{S}}_{{\text{O}}_{\text{2}} }\) levels between 20 and 85%, and there was no saturation dependence of the Bohr factor, its average value being −0.44. It is concluded that in whole blood of this bird species CO2 exerts no direct effect on the O2 affinity of hemoglobin.
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Bartels, H., Christoforides, C., Hedley-Whyte, J., Laasberg, L.: Solubility coefficients of gases. In: Biological handbooks. Respiration and circulation (P. L. Altman and D. S. Dittmer, eds.), pp. 16–19. Bethesda: Federation of the American Societies of Experimental Biology 1971
Bauer, C.: On the respiratory function of haemoglobin. Rev. Physiol. Biochem. Pharmacol.70, 1–31 (1974)
Bauer, C., Jelkmann, W., Rollema, H.: Mechanisms controlling the oxygen affinity of bird and reptile blood. In: Respiratory function in birds, adult and embryonic (J. Piiper, ed.), Berlin-Heidelberg-New York: Springer 1978
Baumann, F. H., Baumann, R.: A comparative study of oxygen transport in bird blood. Respir. Physiol.31, 333–343 (1977)
Benesch, R. E., Benesch, R.: The mechanism of interaction of red cell organic phosphates with hemoglobin. Adv. Protein Chem.28, 211–237 (1974)
Benesch, R., Benesch, R. E., Yu, C. J.: Reciprocal binding of oxygen and diphosphoglycerate by human hemoglobin. Proc. Natl. Acad. Sci. U.S.A.59, 526–532 (1968)
Bohr, C., Hasselbalch, K. A., Krogh, A.: Über einen in biologischer Beziehung wichtigen Einfluß, den die Kohlensäurespannung des Blutes auf dessen Sauerstoffbindung übt. Skand. Arch. Physiol.16, 402–412 (1904)
Dill, D. B., Edwards, H. T., Consolazio, W. V.: Blood as a physicochemical system. XI Man at rest. J. Biol. Chem.118, 635–648 (1937)
Duhm, J.: Dual effect of 2,3-diphosphoglycerate on the Bohr effects of human blood. Pflügers Arch.363, 55–60 (1976)
Garby, L., Robert, M., Zaar, B.: Proton- and carbamino-linked oxygen affinity of normal human blood. Acta Physiol. Scand.84, 482–492 (1972)
Hlastala, M. P., Woodson, R. D.: Saturation dependency of the Bohr effect: interactions among H+, CO2 and DPG. J. Appl. Physiol.38, 1126–1131 (1975)
Holle, J. P., Meyer, M., Scheid, P.: Oxygen affinity of duck blood determined by in vivo and in vitro technique. Respir. Physiol.29, 355–361 (1977)
Isaacks, R. E., Harkness, D. R., Adler, J. L., Goldman, P. H.: Studies on avian erythrocyte metabolism. Effect of organic phosphates on oxygen affinity of embryonic and adult-type hemoglobins of the chick embryo. Arch. Biochem. Biophys.173, 114–120 (1976)
Johnson, L. F., Tate, M. E.: Structure of “phytic acids”. Can. J. Chem.47, 63–73 (1969)
Kawashiro, T., Scheid, P.: Arterial blood gases in undisturbed resting birds: measurements in chicken and duck. Respir. Physiol.23, 337–342 (1975)
Lutz, P. L., Longmuir, I. S., Tuttle, J. V., Schmidt-Nielsen, K.: Dissociation curve of bird blood and effect of red cell oxygen consumption. Respir. Physiol.17, 269–275 (1973)
Meier, U., Böning, D., Rubenstein, H. J.: Oxygenation dependent variations of the Bohr coefficient related to whole blood and erythrocyte pH. Effect of lactic and carbonic acid. Pflügers Arch.349, 203–213 (1974)
Petschow, D., Würdinger, I., Baumann, R., Duhm, J., Braunitzer, G., Bauer, C.: Causes of high blood O2 affinity of animals living at high altitude. J. Appl. Physiol.42, 139–143 (1977)
Rapoport, S., Guest, G. M.: Distribution of acid-soluble phosphorus in the blood cells of various vertebrates. J. Biol. Chem.138, 269–285 (1941)
Scheid, P., Kawashiro, T.: Metabolic changes in avian blood and their effects on determination of blood gases and pH. Respir. Physiol.23, 291–300 (1975)
Scheid, P., Meyer, M.: Mixing technique for the study of oxygen-hemoglobin equilibrium: a critical evaluation. J. Appl. Physiol. (in press, 1978)
Scheipers, G., Kawashiro, T., Scheid, P.: Oxygen and carbon dioxide dissociation of duck blood. Respir. Physiol.24, 1–13 (1975)
Wranne, B., Woodson, R. D., Detter, J. C.: Bohr effect: interaction between H+, CO2 and 2,3-DPG in fresh and stored blood. J. Appl. Physiol.32, 749–754 (1972)
Wyman, J., Jr.: Linked functions and reciprocal effects in hemoglobin: a second look. Adv. Protein Chem.19, 223–286 (1964)
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Meyer, M., Holle, J.P. & Scheid, P. Bohr effect induced by CO2 and fixed acid at various levels of O2 saturation in duck blood. Pflugers Arch. 376, 237–240 (1978). https://doi.org/10.1007/BF00584956
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DOI: https://doi.org/10.1007/BF00584956