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Pflügers Archiv

, Volume 407, Supplement 2, pp S142–S148 | Cite as

A voltage-dependent ionic channel in the basolateral membrane of late proximal tubules of the rabbit kidney

  • H. Gögelein
  • R. Greger
Conductive Pathways

Abstract

The patch-clamp method was applied to the lateral membrane of late proximal tubules of the rabbit kidney. Tubule segments were cannulated on one side by a perfusion system. At the noncannulated end of the tubules, the lateral membrane was accessible to a patch pipette. In cell-attached, as well as cell-excised (presumably inside-out oriented) membrane patches, a voltage sensitive channel was observed. The open-state probability of this channel increased with depolarizing potentials. In cell-excised patches bathed with NaCl-Ringer on both sides, the single channel conductance g was 28.0±1.2 pS (n=10). With KCl-Ringer in the pipette and NaCl-Ringer in the bath g was 24.7±1.3 pS (n=7) and the current-voltage curve crossed the axis at 0 mV. Therefore, the channel does not discriminate between K+ and Na+ ions. Replacing half of NaCl by mannitol on the bath side yielded a permeability for cations about twice as high as for Cl. The channel could be reversibly blocked by diphenylamine-2-carboxylate (DPC), whereas its inhibition by SITS was only partially reversible. In cell-attached patches, the channel was nearly inactivated at zero clamp potential, but became active when the membrane patch was depolarized. The significance of this nonselective channel for proximal tubule cell function is still unclear. It could be involved in the contraluminal exit mechanism of various anions. However, it could also play a role in cell volume regulation processes.

Key words

Late proximal tubule (pars recta) Patch-clamp Basolateral membrane Ionic channel Diphenylamine-2-carboxylate SITS 

References

  1. 1.
    Barac-Nieto M, Murer H, Kinne R (1980) Lactate-sodium cotransport in rat renal brush border membranes. Am J Physiol 239:F496-F506Google Scholar
  2. 2.
    Baumann K, de Rouffignac C, Roinel N, Rumrich G, Ullrich KJ (1975) Renal phosphate transport: inhomogeneity of local proximal transport rates and sodium dependence. Pflügers Arch 356:287–297Google Scholar
  3. 3.
    Bello-Reuss E (1982) Electrical properties of the basolateral membrane of the straight portion of the rabbit proximal renal tubule. J Physiol 326:49–63Google Scholar
  4. 4.
    Blatz AL, Magleby KL (1985) Single chloride-selective channels active at resting membrane potentials in cultured rat skeletal muscle. Biophys J 47:119–123Google Scholar
  5. 5.
    Chesnoy-Marchais D, Evans MG (1984) Two types of chloride channels in outside-out patches from Aplysia neurones. J Physiol 357:64PGoogle Scholar
  6. 6.
    Colquhoun D, Sigworth FJ (1983) Fitting and statistical analysis of single-channel records. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York London, pp 191–264Google Scholar
  7. 7.
    DiStefano A, Wittner M, Schlatter E, Lang HJ, Englert H, Greger R (1985) Diphenylamine-2-carboxylate, a blooker of the Cl-conductive pathway in Cl-transporting epithelia. Pflügers Arch 405:S95-S100Google Scholar
  8. 8.
    Fenwick E, Marty A, Neher E (1982) A patch-clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine J Physiol 331:557–597Google Scholar
  9. 9.
    Fischer RS, Spring KR (1984) Intracellular activities during volume regulation by Necturus gallbladder. J Membr Biol 78:187–199Google Scholar
  10. 10.
    Gögelein H, Greger R (1984) Single channel recordings from basolateral and apical membranes of renal proximal tubules. Pflügers Arch 401:424–426Google Scholar
  11. 11.
    Gögelein H, Greger R (1986) Na+ selective channels in the apical membrane of rabbit later proximal tubules (pars recta). Pflügers Arch 406:198–203Google Scholar
  12. 12.
    Grantham JJ, Lowe CM, Dellasega M, Cole B (1977) Effect of hypotonic medium on K and Na content of proximal renal tubules. Am J Physiol 232:F42-F49Google Scholar
  13. 13.
    Greger R, Gögelein H (1986) Role of K+ conductive pathways in the nephron. Kidney Int (in press)Google Scholar
  14. 14.
    Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391:85–100Google Scholar
  15. 15.
    Hanrahan JW, Alles WP, Lewis SA (1984) Basolateral anion and K channels from rabbit urinary bladder epithelium. J Gen Physiol 84:30aGoogle Scholar
  16. 16.
    Helman SI, Koeppen BM, Beyenbach KW, Baxendale LM (1985) Patch clamp studies of apical membranes of renal cortical collecting ducts. Pflügers Arch 405:71–76Google Scholar
  17. 17.
    Hoffmann N, Thees M, Kinne R (1976) Phosphate transport by isolated renal brush border vesicles. Pflügers Arch 362:147–156Google Scholar
  18. 18.
    Kolb HA, Brown CDA, Murer H (1985) Identification of a voltage-dependent anion channel in the apical membrane of a Cl-secretory epithelium (MDCK). Pflügers Arch 403:262–265Google Scholar
  19. 19.
    Mauyama Y, Petersen OH (1982) Single-channel currents in isolated patches of plasma membrane from basal surface of pancreatic acini. Nature 299:159–161Google Scholar
  20. 20.
    Mueller P, Rudin DO, Tien HT, Wescott WC (1962) Reconstitution of cell membrane structure in vitro and its transformation into an excitable system. Nature 194:979–980Google Scholar
  21. 21.
    Neher E (1982) Unit conductance studies in biological membranes. Techniques in Cellular Physiol P 121:1–6Google Scholar
  22. 22.
    Nelson DJ, Tang JM, Palmer LG (1984) Single-channel recordings of apical membrane chloride conductance in A6 epithelial cells. J Membr Biol 80:81–89Google Scholar
  23. 23.
    Palmer LG, Frindt G (1985) Single channels in the apical membrane of rabbit cortical collecting tubule. Renal Physiol 9:50Google Scholar
  24. 24.
    Rae JL (1984) Single channel recordings from ocular epithelia obtained using a patch voltage clamp. Biophys J 45:385aGoogle Scholar
  25. 25.
    Ullrich KJ, Rumrich G, Klöss S (1982) Reabsorption of monocarboxylic acids in the proximal tubule of the rat kidney. I. Transport kinetics ofd-Lactate, Na+-dependence, pH-dependence and effect of inhibitors. Pflügers Arch 395:212–219Google Scholar
  26. 26.
    Ullrich KJ, Papassilliou F, Rumrich G, Fritzsch G (1985) Contraluminal phosphate transport in the proximal tubule of the rat kidney. Pflügers Arch 405:S106-S109Google Scholar
  27. 27.
    Ullrich KJ, Papavassiliou F (1986) Contraluminal transport of aliphatic small carboxylates in the proximal tubule of the rat kidney in situ. Pflügers Arch (submitted)Google Scholar
  28. 28.
    Sakmann B, Neher E (1983) Geometric parameters of pipettes and membrane patches. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York London, p 37Google Scholar
  29. 29.
    Van Driessche W, Zeiske W (1985) Ionic channels in epithelial cell membranes. Physiol Rev 65:833–903Google Scholar
  30. 30.
    Völkl H, Greger R (1985) Effects of phlorrhizin and temperature changes on basolateral membrane potential on isolated in vitro perfused proximal tubules of mouse kidney. Pflügers Arch 403:R39Google Scholar
  31. 31.
    Wangemann P, Wittner M, DiStefano A, Englert HC, Lang HJ, Schlatter E, Greger E (1985) Cl channel blockers in the thick ascending limb of the loop of Henle. Structure activity relationship. Pflügers Arch 407 (Suppl 2):S128-S141Google Scholar
  32. 32.
    White MM, Miller C (1979) A voltage-gated anion channel from the electric organ of torpedo californica. J Biol Chem 254:10161–10166Google Scholar
  33. 33.
    Yellen G (1982) Single Ca2+-activated nonselective cation channels in neuroblastoma. Nature 296:357–359Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • H. Gögelein
    • 1
  • R. Greger
    • 1
  1. 1.Max-Planck-Institut für BiophysikFrankfurt 70Federal Republic of Germany

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