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Quantitative evaluation of optical reflection spectra of blood-free perfused guinea pig brain using a nonlinear multicomponent analysis

  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Abstract

After developing a blood-free perfused preparation of the guinea pig brain a nonlinear multicomponent analysis (NLMCA) of the reflection spectra of the brain surface was worked out by which the redox state of cytochromeaa 3;c andb could be evaluated quantitatively and almost continuously. The NLMCA allowed to simulate the tissue reflection spectra in such a way that only a small statistical error remained between the recorded and the computed spectra. Using redox states of cytochromeaa 3 andc as an intracellular oxygen probe it was found that in order to supply the guinea brain with sufficient oxygen by a saline medium equilibrated with 95% oxygen and 5% CO2 the temperature had to be reduced to 18–20°C. At this temperature cytochromeaa 3 was 95% oxidized.

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  1. Max-Planck-Institut für Systemphysiologie, Rheinlanddamm 201, D-4600, Dortmund 1, Germany

    U. Heinrich, J. Hoffmann & D. W. Lübbers

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  1. U. Heinrich
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  2. J. Hoffmann
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  3. D. W. Lübbers
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Heinrich, U., Hoffmann, J. & Lübbers, D.W. Quantitative evaluation of optical reflection spectra of blood-free perfused guinea pig brain using a nonlinear multicomponent analysis. Pflugers Arch. 409, 152–157 (1987). https://doi.org/10.1007/BF00584764

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  • DOI: https://doi.org/10.1007/BF00584764

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