Pflügers Archiv

, Volume 396, Issue 1, pp 72–78 | Cite as

Potassium-induced vascular relaxation in two kidney-one clip, renal hypertensive rats

  • R. Clinton Webb
  • David M. Cohen
  • David F. Bohr
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology


This study was designed to characterize potassium-induced relaxation in vascular smooth muscle in two kidneyone clip (2K-1C), renal hypertensive rats. Potassium-induced relaxation was evaluated in the isolated tail artery and in the isolated pump perfused renal vasculature. Both preparations relaxed in response to potassium after contraction induced by norepinephrine in potassium-free solution. Arterial preparations from hypertensive rats showed greater relaxation than did those from normotensive rats. Potassium-induced relaxation in tail arteries from hypertensive rats was more sensitive to ouabain inhibition than those from normotensive rats; the renal vasculature of hypertensive rats did not differ from controls with respect to ouabain sensitivity. Relaxation in response to potassium in isolated tail artery segments varied with the: 1. length of incubation in potassium-free solution; 2. concentration of added potassium; and 3. concentration of norepinephrine added during the potassium-free interval. The amplitude of potassium relaxation is believed to be a functional measure of the electrogenic sodium pump. These experiments support the hypothesis that vascular smooth muscle from 2K-1C renal hypertensive rats has increased electrogenic sodium pump activity, in vitro.

Key words

Electrogenic pump Ouabain Sodium Renal vasculature Tail artery 


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  1. Aprigliano O, Hermsmeyer K (1976) In vitro denervation of the portal vein and caudal artery of the rat. J Pharmacol Exp Ther 198:562–577Google Scholar
  2. Bonaccorsi A, Hermsmeyer K, Aprigliano O, Smith CB, Bohr DF (1977a) Mechanism of potassium relaxation of arterial muscle. Blood Vessels 14:261–276Google Scholar
  3. Bonaccorsi A, Hermsmeyer K, Smith CB, Bohr DF (1977b) Norepinephrine release in isolated arteries induced by K-free solution. Am J Physiol 232:H 140-H 145Google Scholar
  4. Brock T, Overbeck H (1982) Elevated intracellular sodium may not explain increased sodium pump activity in arteries from hypertensive rats.. Hypertension [in press]Google Scholar
  5. Collis MG, Vanhoutte PM (1977) Vascular reactivity of isolated perfused kidneys from male mad female spontaneously hypertensive rats. Circ Res 41:759–767Google Scholar
  6. Friedman SM, Friedman CL (1976) Cell permeability, sodium transport, and the hypertensive process in the rat. Circ Res 39:433–441Google Scholar
  7. Gothberg G, Jandhyala B, Folkow B (1980) Studies on the rate of sodium potassium activated ATPase as determinant of vascular reactivity in Wistar Kyoto and spontaneously hypertensive rats. Clin Sci 59:187s-189sGoogle Scholar
  8. Haddy FJ, Pamnani MB, Clough DL (1980) Volume overload hypertension: A defect on the sodium-potassium pump? Cardiovas Rev Rep 1:376–385Google Scholar
  9. Jones AW, Hart RG (1975) Altered ion transport in aortic smooth muscle during deoxycorticosterone acetate hypertension in the rat. Circ Res 37:333–341Google Scholar
  10. Jones AW, Miller LA (1978) Ion transport in tonic and phasic vascular smooth muscle and changes during deoxycorticosterone hypertension. Blood Vessels 15:83–92Google Scholar
  11. Overbeck HW (1972) Vascular responses to cations, osmolarity and angiotensin in renal hypertensive dogs. Am J Physiol 223:1358–1364Google Scholar
  12. Overbeck HW, Clark OWJ (1975) Vasodilator responses to K+ in genetic hypertensive and in renal hypertensive rats. J Lab Clin Med 86:973–983Google Scholar
  13. Overbeck HW, Grissette, DE (1982) Sodium pump activity in arteries of rats with Goldblatt hypertension. Hypertension 4:132–139Google Scholar
  14. Overbeck HW, Pamnani MB, Akera T, Brody TM, Haddy FJ (1976) Depressed function of ouabain-sensitive sodium-potassium pump from renal hypertensive dog. Circ Res 38 (Suppl II):48–52Google Scholar
  15. Pamnani MB, Clough DL, Haddy FJ (1981) Sodium-potassium pump activity in experimental hypertension. In: Vanhoutte PM, Leusen I (eds) Vasodilatation. Raven Press, New York, pp 391–403Google Scholar
  16. Schwartz A, Lindenmayer GE, Allen JC (1975) The sodium-potassium adenosine triphosphatase: pharmacological, physiological and biochemical aspects. Pharmacol Rev 27:3–134Google Scholar
  17. Suzuki H, Kondo K, Saruta T (1981) Effect of potassium chloride on the blood pressure in two-kidney, one clip Goldblatt hypertensive rats. Hypertension 3:566–573Google Scholar
  18. Webb, RC (1982) Potassium relaxation of vascular smooth muscle from DOCA hypertensive pigs. Hypertension [in press]Google Scholar
  19. Webb RC, Bohr DF (1979) Potassium relaxation of vascular smooth muscle from spontaneously hypertensive rats. Blood Vessels 16:71–79Google Scholar
  20. Webb RC, Bohr DF (1980) Vascular reactivity in hypertension: Altered effect of ouabain. Experientia 36:220–222Google Scholar
  21. Webb RC, Bohr DF (1981) Regulation of vascular tone, molecular mechanisms. Prog Cardiovas Dis 24:213–242Google Scholar
  22. Webb RC, Lockette WE, Vanhoutte PM, Bohr DF (1981) Sodiumpotassium adenosine triphosphatase and vasodilatation. In: Vanhoutte PM, Leusen I (eds) Vasodilatation. Raven Press, New York, pp 319–330Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • R. Clinton Webb
    • 1
  • David M. Cohen
    • 1
  • David F. Bohr
    • 1
  1. 1.Department of PhysiologyUniversity of Michigan Medical SchoolAnn ArborUSA

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