Pflügers Archiv

, Volume 405, Issue 2, pp 170–172 | Cite as

The effect of acute saline volume expansion on renal Na−K-ATPase

  • Michael M. Friedlaender
  • Hanna Wald
  • Mordechai M. Popovtzer
Excitable Tissues and Central Nervous Physiology Short Communication


To examine the role of Na-K-ATPase in the natriuresis that occurs after acute extracellular volume expansion, we performed acute clearance experiments and in vitro analysis of renal microsomal ATPase activity in rats receiving intravenous 0.9% sodium chloride (0.1 ml/100g bw/min). Despite increased absolute reabsorption of filtered sodium (196±8.1 vs. 165±11.4 uEq/min, p<0.05), renal medullary microsomal Na-K-ATPase activity was decreased from 134±5.9 to 110±6.3 pmol Pi/mg protein/hour (p<0.02). No changes occurred in cortical or papillary regions and Mg-ATPase was unaffected. Similar results were obtained after adding 4 mEq/l potassium chloride to the infusion to prevent any fall in serum K+. These data suggest that a considerable percentage of sodium reabsorption is suppressed in acutely volume expanded animals and it is proposed that this is mediated by inhibition of medullary Na-K-ATPase.


Sodium Chloride Potassium Sodium Chloride Human Physiology 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Michael M. Friedlaender
    • 1
  • Hanna Wald
    • 1
  • Mordechai M. Popovtzer
    • 1
  1. 1.Nephrology ServicesHadassah University HospitalJerusalemIsrael

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