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Pflügers Archiv

, Volume 405, Issue 2, pp 108–111 | Cite as

Acetylcholine-induced change in intracellular Cl activity of the mouse lacrimal acinar cells

  • Y. Saito
  • T. Ozawa
  • H. Hayashi
  • A. Nishiyama
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

Using double-barreled Cl-sensitive microelectrodes, intracellular Cl activity (A Cl i ) in the mouse lacrimal acinar cells in vitro was determined in both resting and secretory phases. In the resting stateA Cl i was 31 mmol/l which was 1.4 times higher than that predicted for the passive distribution according to the membrane potential (Vm) of −41 mV. Addition of acetylcholine (ACh, 1μM) hyperpolarizedVm to −63 mV and decreasedA Cl i to 20 mmol/l which was still twice the equilibrium activity. A-23178 produced similar changes inVm andA Cl i to those induced by ACh. It was concluded that Cl was actively accumulated in the acinar cells and, in the secretory phase, Cl efflux was enhanced by the increased driving force and Ca2+-mediated increase in the Cl permeability across the cell membrane.

Key words

Lacrimal gland Cl activity Acetylcholine Cl permeability Ca2+ ionophore 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Y. Saito
    • 1
  • T. Ozawa
    • 1
  • H. Hayashi
    • 2
  • A. Nishiyama
    • 1
  1. 1.Department of PhysiologyTohoku University School of MedicineSendai, MiyagiJapan
  2. 2.College of Medical SciencesTohoku UniversitySendai, MiyagiJapan

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