Pflügers Archiv

, Volume 413, Issue 4, pp 329–335 | Cite as

Glutathione-dependent inactivation of sodium-dependent phosphate transport across rat renal brush-border membrane

  • Makoto Suzuki
  • Takeo Iwamoto
  • Yoshindo Kawaguchi
  • Keiji Iriyama
  • Aiichirou Ogawa
  • Tadashi Miyahara
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


Thiol/disulfide is fundamental in protein function; we previously observed an inhibitory effect of thiol oxidants on the Na-dependent phosphate (Pi) uptake into renal brush border membrane vesicles (BBMV). We examined whether oxidation of glutathione (GSH) is involved in the mechanism. Vesicular thiols were measured by liquid chromatography. BBMV were incubated with reagents before an influx of Pi. Diamide (5 mM) reduced the capacity of the Pi uptake. Subsequent treatment with dithiothreitol (5 mM) blocked the inhibitory effect of diamide. Vesicular GSH was not modified only by the incubation, whereas it was oxidized by the treatment with diamide, and reduced by dithiothreitol. Furthermore, in vivo treatment with cAMP provided GSH-depleted BBMV without any influence on Pi uptake. Diamide did not inhibit the transport of Pi into GSH-depleted vesicles, but it did inhibit the uptake when GSH was introduced into the vesicles. In conclusion, a GSH-dependent mechanism is involved in the inhibitory effect of diamide on sodium-dependent Pi transport across the renal brush-border membrane.

Key words

Renal proximal tubule Phosphate transport Glutathione Thiols 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Makoto Suzuki
    • 1
  • Takeo Iwamoto
    • 2
  • Yoshindo Kawaguchi
    • 1
  • Keiji Iriyama
    • 2
  • Aiichirou Ogawa
    • 1
  • Tadashi Miyahara
    • 1
  1. 1.Second Department of Internal MedicineJikei University School of MedicineTokyoJapan
  2. 2.Division of Biochemistry, Central Research LaboratoryJikei University School of MedicineTokyoJapan

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