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Changes in enzyme activity of glycogen and hexose metabolism during oocyte maturation in a teleost,Misgurnus fossilis L.

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Summary

The oocyte at the end of oogenesis, mature egg and developing embryo of the loachMisgurnus fossilis L.) are characterized by indentical enzyme profiles of the Embden-Meyerhof chain, pentose phosphate cycle and key gluconeogenic enzymes. However, the carbohydrate metabolism in the oocyte differs substantially from that in the embryo.

Oocyte maturation is followed by a complete loss of hexokinase (EC2.7.1.1),2-fold decrease of glycogen synthetase (EC 2.4.1.11) and 10-fold increase of glycogen phosphorylase (EC2.4.1.1) activity. This process is correlated with a gradual decrease of the ATP/(ADP+AMP) ratio from 4∶1 to 2∶1 and increase of the Fructose-6-Phosphate/Fructose-1,6-Diphosphate ratio from 0.27 to 2.0. Thus, oocyte maturation involves a number of changes in control mechanisms resulting in cessation of glycogen accumulation and a transition of carbohydrate metabolism from gluconeogenesis to glycogenolysis.

Zusammenfassung

Die Oozyte und das reife Ei vonMisgurnus fossilis L. besitzen die gleiche Enzymzusammensetzung der Embden-Meyerhof Wege, unterscheiden sich aber wesentlich in den Enzymen des Metabolismus des Glykogens und der Hexosemonophosphate. Beim Reifen der Oozyte erfolgt eine 10fache Zunahme der Aktivität der Phosphorylase (EC 2.4.1.1.), völliger Verlust der Hexokinase (EC 2.7.1.1.) und eine wesentliche Abnahme der Glycogensynthetase (EC 2.4.1.11).

Das Verhältnis ATP/(ADP+AMP)geht von 4∶l auf 2∶-1 zurück, dasjenige von Fructose-6-Phosphat/Fructose-1,6-Diphosphat nimmt von 0,27 auf 2,0 zu.

Änderungen der regulatorischen Mechanismen beim Reifen der Oozyte setzen die Geschwindigkeit der Glykogensynthese und Glukoneogenese scharf herab, unterbinden die Ausnutzung der freien Glukose bei der Glykolyse und sichert eine allmähliche Zunahme der Glykogenolyse während der frühen Entwicklung des Embryos.

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  1. Institute of Developmental Biology, Academy of Sciences of the USSR, Moscow

    Yu. G. Yurowitzky & L. S. Milman

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  1. Yu. G. Yurowitzky
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  2. L. S. Milman
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Yurowitzky, Y.G., Milman, L.S. Changes in enzyme activity of glycogen and hexose metabolism during oocyte maturation in a teleost,Misgurnus fossilis L.. W. Roux' Archiv f. Entwicklungsmechanik 171, 48–54 (1972). https://doi.org/10.1007/BF00584413

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