Pflügers Archiv

, Volume 401, Issue 4, pp 321–323 | Cite as

Levels of blood-bourne factors and cytosol glucocorticoid receptors during the initiation of muscle atrophy in rodent hindlimbs

  • William F. Nicholson
  • Peter A. Watson
  • Frank W. Booth
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology


When only 1 hindlimb of the adult mouse was immobilized for 13 h, the immobilization-induced failure of insulin to stimulate 2-deoxyglucose uptake was observed in the immobilized soleus muscle. 2-deoxyglucose uptake was unchanged in the contralateral, nonimmobilized muscle. In the rat, protein synthesis rates decreased in the one immobilized limb as compared to rates measured in the contralateral nonimmobilized limb of the same animal. The synthesis rates in the contralateral nonimmobilized limb of a rat with one immobilized limb were not significantly different from rates of muscle protein synthesis observed in rats with no hindlimb immobilization. Specific binding of3H-dexamethasone, as determined by exchange assay in the gastrocnemius muscle cytosol, increased after 7 days of immobilization, but not after only 6 h of immobilization. Changes in the level of blood-bourne factors or in cytosolic glucocorticoid levels do not by themselves initiate muscle atrophy in immobilized rodent limbs.

Key words

Muscle protein synthesis Glucocorticoid receptors Glucose uptake Limb immobilization 


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • William F. Nicholson
    • 1
  • Peter A. Watson
    • 1
  • Frank W. Booth
    • 1
  1. 1.Department of Physiology and Cell BiologyThe University of Texas Medical SchoolHoustonUSA

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