Pflügers Archiv

, Volume 401, Issue 4, pp 321–323 | Cite as

Levels of blood-bourne factors and cytosol glucocorticoid receptors during the initiation of muscle atrophy in rodent hindlimbs

  • William F. Nicholson
  • Peter A. Watson
  • Frank W. Booth
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology

Abstract

When only 1 hindlimb of the adult mouse was immobilized for 13 h, the immobilization-induced failure of insulin to stimulate 2-deoxyglucose uptake was observed in the immobilized soleus muscle. 2-deoxyglucose uptake was unchanged in the contralateral, nonimmobilized muscle. In the rat, protein synthesis rates decreased in the one immobilized limb as compared to rates measured in the contralateral nonimmobilized limb of the same animal. The synthesis rates in the contralateral nonimmobilized limb of a rat with one immobilized limb were not significantly different from rates of muscle protein synthesis observed in rats with no hindlimb immobilization. Specific binding of3H-dexamethasone, as determined by exchange assay in the gastrocnemius muscle cytosol, increased after 7 days of immobilization, but not after only 6 h of immobilization. Changes in the level of blood-bourne factors or in cytosolic glucocorticoid levels do not by themselves initiate muscle atrophy in immobilized rodent limbs.

Key words

Muscle protein synthesis Glucocorticoid receptors Glucose uptake Limb immobilization 

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References

  1. 1.
    Booth FW (1977) Time course of muscular atrophy during immobilization of hindlimbs in rats. J Appl Physiol 43:656–661Google Scholar
  2. 2.
    Booth FW, Seider MJ (1979) Early change in skeletal muscle protein synthesis after limb immobilization of rats. J Appl Physiol 47:974–977Google Scholar
  3. 3.
    Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254Google Scholar
  4. 4.
    Clark JH, Peck Jr EJ (1977) Steroid hormone receptors. Basic principles and measurement. In: O'Malley BW, Birnbaumer L (eds) Receptors and hormone action, vol I. Academic, New York, pp 403–409Google Scholar
  5. 5.
    DuBois DC, Almon RR (1980) Disuse atrophy of skeletal muscle is associated with an increase in number of glucocorticoid receptors. Endocrinology 107:1649–1651Google Scholar
  6. 6.
    Garlick PJ, Marshall I (1972) A technique for measuring brain protein synthesis. J Neurochem 19:577–583Google Scholar
  7. 7.
    Garlick PJ, Millward DJ, James WPT (1973) The diurnal response of muscle and liver protein synthesis in vivo in mealfed rats. Biochem J 136:935–945Google Scholar
  8. 8.
    Goldberg AL (1969) Protein turnover in skeletal muscle. J Biol Chem 244:3223–3229Google Scholar
  9. 9.
    Goldspink DF (1977) The influence of immobilization and stretch on protein turnover of rat skeletal muscle. J Physiol 264:267–282Google Scholar
  10. 10.
    LeMarchand-Brustel Y, Jeanrenaud B, Freychet P (1978) Insulin binding and effect in isolated soleus muscle of lean and obese mice. Am J Physiol 234:E348-E358Google Scholar
  11. 11.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  12. 12.
    Nicholson WF, Watson PA, Booth FW (1984) Glucose uptake and glycogen synthesis in muscles from immobilized limbs. J Appl Physiol 56:431–435Google Scholar
  13. 13.
    Rannels SR, Rannels DE, Pegg AE, Jefferson LS (1978) Glucocorticoid effects on peptide-chain initiation in skeletal muscle and heart. Am J Physiol 235:E134-E139Google Scholar
  14. 14.
    Riddick FA, Reisler DM, Kipnis DM (1962) The sugar transport system in striated muscle. Effect of growth hormone, hydrocortisone and alloxan diabetes. Diabetes 11:171–178Google Scholar
  15. 15.
    Seider MJ, Nicholson WF, Booth FW (1982) Insulin resistance for glucose metabolism in disused soleus muscle of mice. Am J Physiol 242:E12-E18Google Scholar
  16. 16.
    Solandt DY, Partridge RC, Hunter J (1942) The effect of skeletal fixation on skeletal muscle. J Neurophysiol 6:17–22Google Scholar
  17. 17.
    Steffen JM, Musacchia XJ (1982) Effect of suspension hypokinesia/hypodynamia on glucocorticoid receptor levels in rat hindlimb muscles. Physiologist 25:151–156Google Scholar
  18. 18.
    Waalkes TP, Undenfriend S (1957) A fluorometric method for the estimation of tyrosine in plasma and tissues. J Lab Clin Med 50:733–736Google Scholar
  19. 19.
    Waterlow JC, Stephen JML (1968) The effect of low protein diets on the turnover rates of serum, liver and muscle proteins in the rat, measured by continuous infusion ofl-[14C] lysine. Clin Sci 35:287–305Google Scholar
  20. 20.
    Waterlow JC, Garlick PJ, Millward DJ (1978) Protein turnover in mammalian tissues and in the whole body. North Holland, Amsterdam p 575Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • William F. Nicholson
    • 1
  • Peter A. Watson
    • 1
  • Frank W. Booth
    • 1
  1. 1.Department of Physiology and Cell BiologyThe University of Texas Medical SchoolHoustonUSA

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