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Pflügers Archiv

, Volume 360, Issue 1, pp 25–44 | Cite as

The hydraulic conductivity of the rat proximal tubular wall determined with colloidal solutions

  • A. E. G. Persson
  • J. Schnermann
  • B. Ågerup
  • N. -E. Eriksson
Article

Summary

The hydraulic conductivity of the rat proximal tubular wall was determined using colloidal solutions perfused in short (50–200 μm) (SMP) or long (90–2000 μm) (LMP) proximal tubular segments. In SMP human serum albumin (HSA) or polyvinylpyrrolidone (PVP) was added to raffinose solutions. AL p of 0.019 nl·min−1·mm−1·mm Hg−1 was found when high colloid concentrations were used while values of 0.055–0.092 were found when low colloid concentrations were used. In other experiments, theL p was determined by perfusing short tubular segments with pure raffinose solutions. A value of 0.015 nl·min−1·mm−1·mm Hg−1 was found. This is twice the value found when raffinose solutions were perfused through long tubular segments and it is concluded that the short microperfusion technique overestimatesL p with a factor of two.

When microperfusions of long tubular segments were conducted, PVP was added to an equilibrium solution consisting of NaCl (110 mM) and raffinose (80 mM).L p was found to be 0.018–0.021 when high colloid concentrations were used, while a value of 0.029 was found when a low colloid concentration was used.

As found in both SMP and LMP a decrease inL p 's with increasing colloid concentrations indicates that a significant influence of radial concentration differences is highly probable. It is therefore suggested that the highestL p derived when using the lowest colloid concentrations represents the best estimate. With thisL p value (0.03–0.05 nl·min−1·mm−1·mm Hg−1) and the existing transtubular hydrostatic and oncotic pressure difference it can be calculated that these passive forces might constitute the driving force for 1/3 of the fluid reabsorbed in the proximal tubule.

Key words

Proximal Tubule Microperfusion Technique Colloidal Solutions Hydraulic Conductance 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • A. E. G. Persson
    • 1
    • 2
  • J. Schnermann
    • 1
    • 2
  • B. Ågerup
    • 1
    • 2
  • N. -E. Eriksson
    • 1
    • 2
  1. 1.Department of Physiologie and Medical Biophysics, Biomedical CenterUniversity of UppsalaSweden
  2. 2.Physiologisches InstitutUniversität MünchenGermany

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