Pflügers Archiv

, Volume 382, Issue 2, pp 145–153 | Cite as

The electrical activity recorded from smooth muscle of the circular layer of the human stomach

  • Yusuke Hara
  • Yushi Ito
Excitable Tissues and Central Nervous Physiology

Abstract

The membrane properties of circular muscles of 55 human stomachs were investigated by microelectrode and double sucrose gap methods. The membrane potential of the circular muscle of the corpus region was —57 mV and no regional difference was evident as compared with tissues from the antrum and cardia. The stomach muscle presented cable like properties, and the length constant measured in the corpus region was 1.34 mm. The circular muscle of all regions of the stomach exhibited slow waves. The amplitude and duration of slow waves varied markedly (the mean values were 18 mV and 6 s, respectively). TheQ10 value for the slow wave was 2.4. The slow wave could be divided into two different components (first and second component) by application of electrical current or by using solutions with various ionic environments. Na ions had more effect on the spike component and Ca ions on the second component. The generation of the first component of the slow wave was blocked by either Na-free, K-free, Ca-free, or Cl-deficient solution but this component reappeared by application of outward current pulse, except in Cl-deficient solution. These results suggest that the generation of slow wave depends on more than one type of ion and that metabolic factors do indeed play a role. Membrane properties of the human stomach were compared with those of the guinea-pig stomach.

Key words

Human stomach Slow wave Electrical property Ionic environment 

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References

  1. Abe, Y., Tomita, T.: Cable properties of smooth muscle. J. Physiol. (Lond.)196, 87–100 (1968)Google Scholar
  2. Boev, K., Golenhofen, K.: Sucrose-gap technique with pressedrubber membranes. Pflügers Arch.349, 277–283 (1974)Google Scholar
  3. Code, C. F., Carlson, H. C.: Motor activity of the stomach. In: Handbook of Physiology, Sect. 6, Vol. 4 (Code, C. F., ed.), pp. 1903–1916. Washington D. C.: American Physiological Society 1968Google Scholar
  4. Connor, J. A., Prosser, C. L., Weems, W. A.: A study of pacemaker activity in intestinal smooth muscle. J. Physiol. (Lond.)240, 671–701 (1974)Google Scholar
  5. Connor, J. A., Kreulen, D., Prosser, C. L., Weigel, R.: Interaction between longitudinal and circular muscle in intestine of cat. J. Physiol. (Lond.)273, 665–689 (1977)Google Scholar
  6. Daniel, E. E., Irwin, J.: Electrical activity of gastric musculature. In: Handbook of Physiology, Sect. 6, Vol. 4, (Code, C. F., ed.), pp. 1969–1984. Washington D.C.: American Physiological Society 1968Google Scholar
  7. El-Sharkawy, T. Y., Morgan, K. G., Szurszewski, J. H.: Intracellular electrical activity of canine and human gastric smooth muscle. J. Physiol. (Lond.)279, 291–307 (1978)Google Scholar
  8. El-Sharkawy, T. Y., Szurszewski, J. H.: Modulation of canine antral circular smooth muscle by acetylcholine, noradrenaline and pentagastrin. J. Physiol. (Lond.)279, 309–320 (1978)Google Scholar
  9. Golenhofen, K.: Slow rhythms in smooth muscle (minute-rhythm). In: Smooth muscle (Bülbring, E., Brading, A., Jones, A., Tomita, T., eds.), pp. 316–342. London: Arnold 1970Google Scholar
  10. Golenhofen, K.: Spontaneous activity and functional classification of mammalian smooth muscle. In: Physiology of smooth muscle, (Bülbring, E., Shuba, M. F., eds.), pp. 91–97. New York: Raven Press 1976Google Scholar
  11. Hinder, R. A., Kelly, K. A.: Human gastric pacesetter potential. Am. J. Surg.133, 29–33 (1977)Google Scholar
  12. Ito, Y., Suzuki, H., Kuriyama, H.: Effects of caffeine and procaine on the membrane and mechanical properties of the smooth muscle cells of the rabbit main pulmonary artery. Jpn. J. Physiol.27, 467–481 (1977)Google Scholar
  13. Job, D. D.: Ionic basis of intestinal electrical activity. Am. J. Physiol.217, 1534–1541 (1969)Google Scholar
  14. Kelly, K. A.: Gastric motility after gastric operation. Surg. Ann.6, 103–123 (1974)Google Scholar
  15. Kuriyama, H., Osa, T., Tasaki, H.: Electrophysiological studies of the antrum muscle fibers of the guinea pig antrum. J. Gen. Physiol.55, 48–62 (1970)Google Scholar
  16. Kuriyama, H., Suzuki, H.: Changes in electrical properties of rat myometrium during gestation and following hormonal treatments. J. Physiol. (Lond.)260, 315–333 (1976)Google Scholar
  17. Liu, J., Prosser, C. L., Job, D. D.: Ionic dependence of slow waves and spikes in intestinal muscle. Am. J. Physiol.217, 1542–1547 (1969)Google Scholar
  18. Magaribuchi, T., Ohbu, T., Sakamoto, Y., Yamamoto, Y.: Some electrical properties of the slow potential changes recorded from the guinea-pig stomach in relation to drug action. Jpn J. Physiol.22, 333–352 (1972)Google Scholar
  19. Ohba, M., Sakamoto, Y., Tomita, T.: The slow wave in the circular muscle of the guinea-pig stomach. J. Physiol. (Lond.)253, 505–516 (1975a)Google Scholar
  20. Ohba, M., Sakamoto, Y., Tomita, T.: Spontaneous rhythmic activity of the smooth muscle of the guinea-pig stomach and effects of ionic environment. In: Smooth muscle pharmacology and physiology, (Worgel, M., Vassort, G., eds.), pp. 301–316. Paris: INSERM 1975bGoogle Scholar
  21. Ohba, M., Sakamoto, Y., Tomita, T.: Effects of sodium, potassium and calcium ions on the slow wave in the circular muscle of the guinea-pig stomach. J. Physiol. (Lond.)267, 167–180 (1977)Google Scholar
  22. Papasova, M. P., Nagai, T., Prosser, C. L.: Tow component slow waves in smooth muscle of cat stomach. Am. J. Physiol.214, 695–702 (1968)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Yusuke Hara
    • 1
  • Yushi Ito
    • 1
  1. 1.Department of Pharmacology, Faculty of MedicineKyushu UniversityFukuokaJapan

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