Abstract
Using intracellular microelectrode technique, the effect of anion substitution on the voltage responses to extracellular bicarbonate and sodium was explored in cultured bovine corneal endothelial cells.
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1.
The overall amplitude of voltage changes induced by periodic changes of [HCO −3 ]0 (depolarization upon removal of HCO −3 and hyperpolarization upon readdition) was reduced when Cl− was replaced by organic anions (cyclamate, methylsulfate, benzenesulfonate) or by SO 2−4 , and to a lesser extent by substitution with Br−.
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2.
There was a similar effect of anion substitution on the response to changes of [Na+]0.
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3.
In both cases, in the absence of Cl, the voltage V returned at a slower rate to baseline levels after it had been transiently changed by either an imposed Na- or HCO3-gradient, indicating a slower dissipation of these gradients. The direct response of V to these imposed gradients was affected only to a minor degree.
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4.
Replacement of Cl− by SO 2−4 or organic anions led to a slow, reversible depolarization of the cell, while substitution with Br− had only a slight effect.
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5.
The effect of anion substitution on the voltage responses to HCO −3 or Na+ could not be mimicked by a depolarization induced by Ba2+ (1 mM).
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6.
Furosemide (10−3 M) led to a slight reduction of the voltage responses to HCO −3 , but could not suppress the effect of anion substitution on these reactions. It could neither suppress the depolarization induced by anion substitution and had no effect on steady-state PD.
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7.
It is suggested, that cultured bovine corneal endothelial cells, in addition to a previously demonstrated electrogenic HCO −3 −Na+-cotransport, which is probably not dependent on Cl, possess an electroneutral mechanism for HCO −3 and/or Na+-movement, which depends on Cl. No evidence for a Cl-conductance could be obtained.
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Part of this work has been published previously in abstract form [29]
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Jentsch, T.J., Matthes, H., Keller, S.K. et al. Anion dependence of electrical effects of bicarbonate and sodium on cultured bovine corneal endothelial cells. Pflugers Arch. 403, 175–185 (1985). https://doi.org/10.1007/BF00584097
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DOI: https://doi.org/10.1007/BF00584097