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Lack of PCMB action upon the outer barrier sodium permeability in the absence of Na in toad skin

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

The stimulation of apical Na permeability by p-chloromercuribenzoate (PCMB) was evaluated by studying its effect on unidirectional24Na flux (Jeff Na) from inner to outer compartment in the isolated short-circuited toad skin.

With Na present in the inner compartment, addition of PCMB to the outer skin surface led to an increase of Jeff Na. However, after replacing all Na of the inner compartment by K or choline, the amiloride inhibitable Na permeability of the outer cell membrane increased, and PCMB was no longer effective. It is concluded that PCMB releases the blocking effect of intracellular Na on apical Na channels. If intracellular Na is drastically reduced virtually all Na channels are opened so that PCMB does no longer act.

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Authors and Affiliations

  1. Institute of Biomedical Sciences, Department of Physiology and Biophysics, University of São Paulo, 05508, São Paulo, SP, Brazil

    S. M. L. Sanioto

  2. Dept. of Biophysics, Escola Paulista de Medicina, CP 20338, 04034, São Paulo, SP, Brazil

    J. Aboulafia

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  1. S. M. L. Sanioto
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  2. J. Aboulafia
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Sanioto, S.M.L., Aboulafia, J. Lack of PCMB action upon the outer barrier sodium permeability in the absence of Na in toad skin. Pflugers Arch. 403, 115–119 (1985). https://doi.org/10.1007/BF00584087

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  • DOI: https://doi.org/10.1007/BF00584087

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