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Pflügers Archiv

, Volume 409, Issue 4–5, pp 462–467 | Cite as

Cultivation, morphology, and electrophysiology of contractile rat myoballs

  • S. Boldin
  • U. Jäger
  • J. P. Ruppersberg
  • S. Pentz
  • R. Rüdel
Excitable Tissues and Central Nervous Physiology

Abstract

Myoballs were cultured from neonatal rat skeletal muscle without the use of antimitotic drugs. Electron microscopic investigation showed that 7-day-old myoballs are multinucleated syncytia in a state of differentiation where filaments are abundant and already in hexagonal arrays. The resting potential of 142 myoballs kept at 20°C was not correlated with the cell size. Its mean value was −64 mV. Cells with a high resting potential were capable of generating action potentials with a threshold of −51 mV, an overshoot of +31 mV, and a rate of rise of 100 V/s. The steady-state current-voltage relation showed inward rectification on hyperpolarization and outward rectification on depolarization. The dynamic sodium and potassium currents were investigated at 37°C with the whole-cell-recording technique. The sodium current had its maximum at −20 mV. The potassium current showed delayed activation and a very slow and incomplete inactivation. The electrophysiological results from these cultured cells are very similar to those obtained from adult cells.

Key words

Myoballs Muscle culture Current-voltage relation Whole-cell patch-clamp Na+- and K+ currents 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • S. Boldin
    • 1
  • U. Jäger
    • 1
  • J. P. Ruppersberg
    • 1
  • S. Pentz
    • 2
  • R. Rüdel
    • 1
  1. 1.Abteilung für Allgemeine Physiologie der Universität UlmUlmGermany
  2. 2.Abteilung für Klinische Genetik der Universität UlmUlmGermany

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